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The kinetics of glucose
production from rice straw by Aspergillus niger
B. O. Aderemi1*, E. Abu2 and B. K.
Highina3
1Department
of Chemical Engineering, Ahmadu
Bello University, Zaria, Nigeria.
2Department
of Biochemistry, Ahmadu Bello
University, Zaria, Nigeria.
3Department
of Chemical Engineering, University of Maiduguri, Maiduguri,
Nigeria.
*Corresponding author. E-mail:
elfemdor@yahoo.com.
Accepted
2 May, 2008 |
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In this
investigation, glucose was produced from rice straw using
cells of Aspergillus niger, isolated from maize
grain. Glucose yield was found to increase from 43 to 87% as
the rice straw particle size decreased from 425 to 75 µm,
while the optimal temperature and pH were found within the
range of 45 - 50°C and 4.5 - 5 respectively. The
concentration and rate of glucose production was observed to
depend on pretreatment of rice straw, substrate
concentration and cell loading.
A kinetic
model rate expression has been developed for such a process
based on the Michaelis – Mentens and Lineweaver – Burk
approach. Comparison between the experimental data and those
predicted from the rate model indicate good agreement with a
mean absolute deviation of about 0.2.
Key
words:
Rice straw, cellulose, glucose, Aspergillus niger,
enzyme loading, kinetic parameters, kinetic model. |
