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Effects of morphogenetic
and diurnal variability on the hypericin content in St.
John’s Wort (Hypericum perforatum L.)
Oya Kaçar*,
Erdinç Göksu and Nedime Azkan
Uludag
University, Faculty of Agriculture, Department of Field
Crops, Bursa, Turkey.
*Corresponding author. E-mail:
okacar@uludag.edu.tr.
Tel: +90 224 2941523. Fax: +90 224 442 88 36.
Accepted
2 June, 2008 |
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This research was conducted to investigate effects of
morphogenetic and diurnal variability on the hypericin
content of St. John’s Wort (Hypericum perforatum L.)
populations originating from Turkey for maximum utilization
of the active substance in plants. During 2002 and 2003,
field trials were conducted at Uludag University, Faculty of
Agriculture, Department of Field Crops, Bursa, Turkey.
Samples of bud, flower and capsule of H. perforatum
L. populations were collected in the second year of
cultivation (2003). Factorial arrangements of three H.
perforatum L. populations (Bursa, Edirne, Izmir), three
part of plant (bud, flower, capsule) and six collection h
(8:00, 10:00 a.m; 12:00 (noon), 2:00, 4:00, 6:00 p.m) were
evaluated in a completely randomized block design with three
replications. Hypericin content (%) in parts of H.
perforatum L. populations was determined according to
DAC (1986). Consequently, the content of hypericin in the
examined populations varied from 0.260% in Bursa to 0.283%
in Izmir. Evaluating plant parts revealed that the hypericin
content both in floral parts (0.309%) and buds (0.308%) were
higher than capsule tissues (0.208%) for all populations.
When collection hours were examined for the hypericin
content of plants, the highest content was recorded at 10:00
a.m (0.279%) and the lowest value at 4:00 p.m (0.272%) and
6:00 p.m (0.272%). As a result, this study showed that the
highest hypericin ratio was determined in flowers and buds
generally collected between 8:00 and 10:00 a.m within a day
for examined Hypericum populations.
Key
words:
St. John’s Wort, Hypericum perforatum L.,
morphogenetic variability, diurnal variability, hypericin
content. |
