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Effect of different plant
hormones on callus induction in Gymnema sylvestris
R.Br. (Asclepiadaceae)
Amitava Roy1,
Shayak Ghosh1, Madhulina Chaudhuri 1
and P. K. Saha2
1Plant
Tissue Culture and Biotechnology Section, Department of
Botany, Scottish Church College, 1 Urquhart Square,
Kolkata-700006 India.
2Department
of Botany, Bose Institute, 93/1 A.P.C. Road Kolkata 700009
India.
*Corresponding authors. E-mail:
pksaha@bosemain,
boseinst.ac.in. Tel: 91+[033] 2303 1126.
Abbreviations: IAA, Indole acetic acid; 2,4-D,
2,4-dichlorophenoxyacetic acid; KIN, kinetin; BAP,
benzylaminopurine; GA, gymnemic acid.
Accepted
3 June, 2008 |
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The use of Gymnema sylvestris as an alternative remedy
for diabetes was well known from ancient times in India and
the plant is valuable for its pharmaceutical properties. The
present study describes callus induction of the plant G.
sylvestris using internodal explants and the influence
of different plant hormones like 2,4-D, kinetin, IAA, BAP on
the growth of calli. Internodes were proved to be the best
explant for culture, which were grown on MS basal medium (Murashige
and Skoog, 1962) with different concentration of various
growth regulators. The standard plant tissue culture
protocol for callus culture and/or micropropagation was
adopted. The highest efficiency of callus formation was
observed in the medium containing different concentration of
2,4-D and kinetin.
Key
words:
Gymnema sylvestris, tissue culture, micropropagation,
plant growth regulators, callus. |
