African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 5816

Full Length Research Paper

Effects of associated co-inoculation of Bradyrhizobium japonicum with Azospirillum brasilense on soybean yield and growth

Glaucia Cristina Ferri
  • Glaucia Cristina Ferri
  • Graduate Program in Agronomy, State University of Maringá, Brazil.
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Alessandro Lucca Braccini
  • Alessandro Lucca Braccini
  • Department of Agronomy, State University of Maringá, Brazil.
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Fernanda Brunetta Godinho Anghinoni
  • Fernanda Brunetta Godinho Anghinoni
  • Graduate Program in Agronomy, State University of Maringá, Brazil.
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Lucas Caiubi Pereira
  • Lucas Caiubi Pereira
  • Graduate Program in Agronomy, State University of Maringá, Brazil.
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  •  Received: 15 September 2016
  •  Accepted: 31 October 2016
  •  Published: 05 January 2017

Abstract

The continuous development of soybean cultivars aiming at higher yields can result in a demand of N supply higher than the standard inoculation with Bradyrhizobium species. In this context, Azospirillum species, a nitrogen-fixing microorganism found in the rhizosphere of various plant species, may be studied as a way of providing soybean crop with the part of the N it needs. Employing a completely randomized design with four replicates, this study aimed to evaluate the agronomic performance of two different methods of standard inoculation of Bradyrhizobium spp. associated with the co-inoculation with Azospirillum spp. The tested treatments consisted of the absence of N fertilizer and inoculant, full N recommendation, two single inoculations with Bradyrhizobium spp. (in-furrow and seed-applied) combined with an in-furrow application of Azospirillum spp. At R2 growth stage, the nodule number, nodule dry weight, shoot dry biomass and shoot N content were the variables evaluated, whereas at R8 the grain yield, thousand seed weight and grain N content were assessed. The results demonstrated that the co-inoculation of Bradyrhizobium spp. with Azospirillum brasilense is beneficial to soybean yield, mainly using the in-furrow method of application for Bradyrhizobium spp., associated to the in-furrow co-inoculation with Azospirillum spp.

 

Key words: Glycine max, inoculation, nitrogen content, yield.