African Journal of Agricultural Research
Subscribe to AJAR
Full Name*
Email Address*

Article Number - 32CC5CC62225


Vol.12(1), pp. 6-11 , January 2017
DOI: 10.5897/AJAR2016.11711
ISSN: 1991-637X



Full Length Research Paper

Effects of associated co-inoculation of Bradyrhizobium japonicum with Azospirillum brasilense on soybean yield and growth



Glaucia Cristina Ferri
  • Glaucia Cristina Ferri
  • Graduate Program in Agronomy, State University of Maringá, Brazil.
  • Google Scholar
Alessandro Lucca Braccini
  • Alessandro Lucca Braccini
  • Department of Agronomy, State University of Maringá, Brazil.
  • Google Scholar
Fernanda Brunetta Godinho Anghinoni
  • Fernanda Brunetta Godinho Anghinoni
  • Graduate Program in Agronomy, State University of Maringá, Brazil.
  • Google Scholar
Lucas Caiubi Pereira
  • Lucas Caiubi Pereira
  • Graduate Program in Agronomy, State University of Maringá, Brazil.
  • Google Scholar







 Received: 15 September 2016  Accepted: 31 October 2016  Published: 05 January 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The continuous development of soybean cultivars aiming at higher yields can result in a demand of N supply higher than the standard inoculation with Bradyrhizobium species. In this context, Azospirillum species, a nitrogen-fixing microorganism found in the rhizosphere of various plant species, may be studied as a way of providing soybean crop with the part of the N it needs. Employing a completely randomized design with four replicates, this study aimed to evaluate the agronomic performance of two different methods of standard inoculation of Bradyrhizobium spp. associated with the co-inoculation with Azospirillum spp. The tested treatments consisted of the absence of N fertilizer and inoculant, full N recommendation, two single inoculations with Bradyrhizobium spp. (in-furrow and seed-applied) combined with an in-furrow application of Azospirillum spp. At R2 growth stage, the nodule number, nodule dry weight, shoot dry biomass and shoot N content were the variables evaluated, whereas at R8 the grain yield, thousand seed weight and grain N content were assessed. The results demonstrated that the co-inoculation of Bradyrhizobium spp. with Azospirillum brasilense is beneficial to soybean yield, mainly using the in-furrow method of application for Bradyrhizobium spp., associated to the in-furrow co-inoculation with Azospirillum spp.

 

Key words: Glycine max, inoculation, nitrogen content, yield.

AOAC (2000). Association of Official Analytical Chemists. Official methods of analysis of A.O.A.C Internacional. 17 th ed. Gaitherburg, 1-2.

 

Araújo EO, Mercante FM, Vitorino ACT (2015). Effect of nitrogen fertilization associated with inoculation of Azospirillum brasilense and Herbaspirillum seropedicae on corn. Afr. J. Agric. Res. 10(3):137-145.
Crossref

 
 

Banzatto DA, Kronka SN (2008). Experimentação Agrícola. 4 th ed Jaboticabal: FUNEP 237p.

 
 

Bashan Y, Dubrovsky JG (1996). Azospirillum spp. participation in dry matter partitioning in grasses at the whole plant level. Biol. Fertil. Soils 23:435-440.
Crossref

 
 

Bashan Y, De-Bashan LE (2010). How the plant growth-promoting bacterium Azospirillum promotes plant growth - a critical assessment 2010. Adv. Agron. 108:77-136.
Crossref

 
 

Bashan Y, Holguin G, De-Bashan LE (2004). Azospirillum-plant relations physiological, molecular, agricultural, and environmental advances (1997-2003). Can. J. Microbiol. 50:521-577.
Crossref

 
 

Biari A, Gholami A, Rahmani HA (2008). Growth promotion and enhanced nutrient uptake of maize (Zea mays L.) by application of plant prowth promoting rhizobacteria in arid region of Iran. J. Biol. Sci. 8(6):1015-1020.
Crossref

 
 

Brandão Junior O, Hungria M (2000). Efeito de doses de inoculante turfoso na fixação biológica do nitrogênio pela cultura da soja. Rev. Bras. Ciênc. Solo 24(3):527-535.
Crossref

 
 

Brasil (2009). Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Brasília: MAPA/ACS. 399p.

 
 

Caviglione JH, Kiihll LRB, Caramori PH, Oliveira D (2000). Cartas climáticas do Paraná. Londrina: IAPAR, CD.

 
 

Costa RRGF, Quirino GSF, Naves DCF, Santos CB, Rocha AFS (2015). Efficiency of inoculant with Azospirillum brasilense on the growth and yield of second-harvest maize. Pesq. Agropec. Trop. 45(3):304-311.
Crossref

 
 

Dall'Agnol A, Lazarotto JJ, Hirakuri MH (2010) Desenvolvimento, mercado e rentabilidade da soja brasileira. Circular Técnica 74. Embrapa Soja. Londrina.

 
 

Dobbelaere S, Croonenborghs A, Trys A, VandeBroek A, Vanderleyden J (1999). Phytostimulatory effect of Azospirillum brasilense wild type and mutant strains altered in IAA production on wheat. Plant Soil 212:155-164.
Crossref

 
 

Dobbelaere S, Vanderleyden J, Okon Y (2003). Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit. Rev. Plant Sci. 22:107-149.
Crossref

 
 

Domingues Neto FJ, Yoshimi FK, Garcia RDG, Miyamoto YR, Domingues MCS (2013). Desenvolvimento e produtividade do milho verde safrinha em resposta à aplicação foliar com Azospirillum brasilense. Enciclopédia Biosfera 9(17):1030-1040.

 
 

Embrapa: Empresa Brasileira de Pesquisa Agropecuária (2011). Tecnologias de produção de soja – região central do Brasil 2012 e 2013. Londrina: Embrapa Soja, 264 p. (Sistemas de Produção, 15).

 
 

Embrapa: Empresa Brasileira de Pesquisa Agropecuária (2013). Sistema brasileiro de classificação de solos. Embrapa Solos. Rio de Janeiro, Brasil. 306 p.

 
 

Ferreira DF (2011). Sisvar: a computer statisticalanalysis system. Ciênc. Agrotec. 35(6):1039-1042.

 
 

Hallmann J, Quadt–Hallmann A, Mahaffee WF, Kloepper JW (1997). Bacterial endophytes in agricultural crops. Can. J. Microb. 43:895-914.
Crossref

 
 

Hawkesford M, Horst W, Kichey T, Lambers H, Schjoerring J, Skrumasager I (2012). Functions of macronutrients. In: Marschner P (ed.). Marschner's mineral nutrition of higher plants. 3rd ed. London: Elsevier. pp. 135-178.
Crossref

 
 

Hungria M, Campo RJ, Mendes IC (2007). A importância do processo de fixação biológica de nitrogênio para a cultura da soja: componente essencial para a competitividade do produto brasileiro. Londrina, Embrapa Soja, 80 p (Documentos, 283).

 
 

Hungria M, Campo RJ, Souza EM, Pedrosa FO (2010). Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant Soil 331:413-425.
Crossref

 
 

Hungria M, Franchini JC, Campo RJ, Crispino CC, Moraes JZ, Sibaldelli RNR, Mendes IC, Arihara J (2006). Nitrogen Nutrition of Soybean in Brazil: Contributions of Biological N2 Fixation and of N Fertilizer to Grain Yield. Can. J. Plant Sci. 86:927-939.
Crossref

 
 

Hungria M, Nogueira MA, Araujo R (2015). Soybean seed coinoculation with Bradyrhizobium spp. and Azospirillum brasilense: a new biotechnological tool to improve yield and sustainability. Am. J. Plant Sci. 6:811-817.
Crossref

 
 

Hungria M, Nogueira MA, Araujo RS (2013). Tecnologia de coinoculação da soja com Bradyrhizobium e Azospirillum: incrementos no rendimento com sustentabilidade e baixo custo. In: Reunião de Pesquisa de Soja da Região Central do Brasil 33, Londrina.

 
 

Mark GL, Dow JM, Kiely PD, Higgins H, Haynes J, Baysse C, O'Gara F (2005). Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions. Proc. Nat. Acad. Sci. United States Am. 102(48):17454-17459.
Crossref

 
 

Martinez HEP, Carvalho JG, Souza RB (1999). Diagnose foliar. In: Ribeiro AC, Guimarães PTG, Alvarez VVH (eds) Recomendação para uso de corretivos e fertilizantes em Minas Gerais: 5a Aproximação. Viçosa, Comissão de Fertilidade do Solo do Estado de Minas Gerais. pp. 143-168.

 
 

Mendes IC, Hungria M, Vargas MAT (2003). Soybean response to starter nitrogen and Bradyrhizobium inoculation on a Cerradooxisol under no-tillage and conventional tillage system. Rev. Bras. Ciênc. Solo 27:81-87.
Crossref

 
 

Morais TP, Brito CH, Brandão AM, Rezende WS (2016). Inoculation of maize with Azospirillum brasilense in the seed furrow. Rev. Ciênc. Agron. 47(2):290-298.

 
 

Novakowiski JH, Sandini IE, Falbo MK, de Moraes A, Novakowiski JH, Cheng NC (2011). Efeito residual da adubação nitrogenada e inoculação de Azospirillum brasilense na cultura do milho. Semin. Ciênc. Agrar. 32:1687-1698.
Crossref

 
 

Pedraza RO, Bellone CH, de Bellone S, Sorte PMB, Teixeira KRD (2009). Azospirillum inoculation and nitrogen fertilization effect on grain yield and on the diversity of endophytic bacteria in the phyllosphere of rice rainfed crop. Eur. J. Soil Biol. 45:36-43.
Crossref

 
 

Petersen R (1994). Agricultural field experiments: Design and analysis. Marcel Dekker Inc., New York, USA.426 p.

 
 

Saubidet MI, Fatta N, Barneix AJ (2002). The effect of inoculation with Azospirillum brasilense on growth and nitrogen utilization by wheat plants. Plant Soil 245:215-222.
Crossref

 
 

Taiz L, Zeiger E (2004). Fisiologia Vegetal.Trad. Santarém, E R et al., 3. ed. Porto Alegre: Artmed. 719p.

 
 

USDA (2016). United State Department of Agriculture – Foreign Agricultural Service. Available on: 

View. Acessed on: 02 September 2016.

 
 

Veloso CAC, Viégas I de JM, Oliveira RF de, Botelho SM (2006). Amostragem de solo e planta para análise química. Belém, PA: Embrapa Amazônia Oriental. 39p.

 
 

Vitti GC, Camargo MAF, Lara C (2001). Síntese de análise químicas em tecido vegetal. Piracicaba: Departamento de Solos e Nutrição de Plantas, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo.

 
 

Zilli JE, Marson LC, Campo RJ, Gianluppi V, Hungria M (2006). Avaliação da fixação biológica de nitrogênio na soja em áreas de primeiro cultivo no cerrado de Roraima. Embrapa Roraima. 9p. (Comunicado Técnico, 20).

 

 


APA Ferri, G. C., Braccini, A. L., Anghinoni, F. B. G., & Pereira, L. C. (2017). Effects of associated co-inoculation of Bradyrhizobium japonicum with Azospirillum brasilense on soybean yield and growth. African Journal of Agricultural Research, 12(1), 6-11.
Chicago Glaucia Cristina Ferri, Alessandro Lucca Braccini, Fernanda Brunetta Godinho Anghinoni and Lucas Caiubi Pereira. "Effects of associated co-inoculation of Bradyrhizobium japonicum with Azospirillum brasilense on soybean yield and growth." African Journal of Agricultural Research 12, no. 1 (2017): 6-11.
MLA Glaucia Cristina Ferri, et al. "Effects of associated co-inoculation of Bradyrhizobium japonicum with Azospirillum brasilense on soybean yield and growth." African Journal of Agricultural Research 12.1 (2017): 6-11.
   
DOI 10.5897/AJAR2016.11711
URL http://academicjournals.org/journal/AJAR/article-abstract/32CC5CC62225

Subscription Form