African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 5816

Full Length Research Paper

Genetic divergence in Agave accessions through ISSR markers and phenotypic traits

Silmara Chaves de Souza
  • Silmara Chaves de Souza
  • Postgraduate in Agrarian Sciences, Universidade Estadual da Paraíba, Rua Baraúnas, 351, Bairro Universitário, Campina Grande-PB, CEP 58429-500, Brazil.
  • Google Scholar
Jose Jaime Vasconcelos Cavalcanti
  • Jose Jaime Vasconcelos Cavalcanti
  • Embrapa Algodão, R. Osvaldo Cruz, 1143, Centenário, Campina Grande-PB, 58428-095, Brazil.
  • Google Scholar
Jean Pierre Cordeiro Ramos
  • Jean Pierre Cordeiro Ramos
  • Postgraduate in Agronomy, Universidade Federal da Paraíba, Campus II, Rodovia PB 079- km 12, Areia-PB, CEP 58397-000, Brazil.
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Isaías Alves
  • Isaías Alves
  • Embrapa Algodão, R. Osvaldo Cruz, 1143, Centenário, Campina Grande-PB, 58428-095, Brazil.
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Roseane Cavalcanti dos Santos
  • Roseane Cavalcanti dos Santos
  • Embrapa Algodão, R. Osvaldo Cruz, 1143, Centenário, Campina Grande-PB, 58428-095, Brazil.
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Liziane Maria de Lima
  • Liziane Maria de Lima
  • Embrapa Algodão, R. Osvaldo Cruz, 1143, Centenário, Campina Grande-PB, 58428-095, Brazil.
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  •  Received: 30 November 2017
  •  Accepted: 23 January 2018
  •  Published: 15 March 2018

Abstract

The Agave genus is composed of about 200 species, but the cultivation of sisal for fiber production in Brazil is restricted to two species: Agave sisalana and Agave fourcroydes, both have several accessions with wide variability. The collection of Agave of Embrapa has 37 accessions maintained in situ and periodically evaluated agronomical traits. Most of these accessions have phenotypic similarities, although they differ in fiber quality, which are widely used for commercial purposes. The identification of promising accesses contributes to the advance in improvement works, focusing on commercial indication. In order to estimate the genetic divergence of this collection, a cluster analyses was performed based on Inter-Simple Sequence Repeat (ISSR) markers and phenotypic traits. Genomic DNA from these accessions were used in polymerase chain reaction (PCR) with thirty ISSR oligonucleotides. For phenotypic characterization, twelve descriptors were adopted based on morphological and agronomic data. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and Tocher multivariate methods were adopted. Thirteen groups were formed by the Tocher Method and six by UPGMA; however UPGMA method was more representative in the group formation. The comparison of the band patterns among accessions derived from the shoots showed that genetic variability is generated during asexual reproduction in these plants. The four lines generated from Tatui were the most divergent accessions. These plants are tallest, with higher mass values of fresh and dry mucilage, fresh and dry fiber mass, fiber length and presence of spines at the edges. The accessions from Instituto Agronômico de Campinas (IAC) showed the lowest genetic distances, indicating a possible narrow genetic base and high kinship degree. The crossings between H-RN, H-Kenya, H-400 fls, and H-11648 with Tatuí 1, 2, 3, and 4 can be a valorous strategy to broadening genetic diversity among commercial and native sisal germplasm.

Key words: Sisal, Agave sisalana, molecular markers, morphoagronomic, diversity, cropping breeding.