African Journal of Agricultural Research
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Article Number - B17770966586


Vol.12(44), pp. 3203-3213 , November 2017
DOI: 10.5897/AJAR2017.12617
ISSN: 1991-637X



Full Length Research Paper

Genetic diversity among muskmelon (Cucumis melo L.) germplasm in Bangladesh as revealed by microsatellite markers



Md. Rezwan Molla
  • Md. Rezwan Molla
  • Molecular Biology Laboratory, Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.
  • Google Scholar
Iftekhar Ahmed
  • Iftekhar Ahmed
  • Molecular Biology Laboratory, Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.
  • Google Scholar
Sajia Rahman
  • Sajia Rahman
  • Molecular Biology Laboratory, Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.
  • Google Scholar
Md. Amjad Hossain
  • Md. Amjad Hossain
  • Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh.
  • Google Scholar
Md. Abdus Salam
  • Md. Abdus Salam
  • Crops Division, Bangladesh Agricultural Research Council, Farmgate, Dhaka-1215 Bangladesh.
  • Google Scholar
Md. Aziz Zilani Chowdhury
  • Md. Aziz Zilani Chowdhury
  • Crops Division, Bangladesh Agricultural Research Council, Farmgate, Dhaka-1215 Bangladesh.
  • Google Scholar
Md. Motiar Rohman
  • Md. Motiar Rohman
  • Molecular Breeding Laboratory, Plant Breeding Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.
  • Google Scholar







 Received: 25 July 2017  Accepted: 11 September 2017  Published: 02 November 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Information about genetic variation has become more critical and deciding factor for any breeding and improvement effort,is difficult inefficient and inaccurate when based on morphological traits only. Therefore, this investigation was conducted using nine microsatellite DNA markers to evaluate genetic variation among 96 muskmelon germplasm in Bangladesh. All nine microsatellite markers were polymorphic. Lower values detected in observed than expected heterozygosity for all loci, indicating homozygous condition in the sample population. A total number of 28 alleles were generated by the nine primers across 96 germplasm. The locus TJ10 showed the highest number of alleles (5), whereas CMGA104, CMCT44, CMAG59, CMTA134a and J27 were generated lowest (3) number of alleles.Allele size ranged between 98 bp (CMCT44) to 198 (CMCTT144). The Polymorphism Information Content (PIC) valuesranged from 0.117 to 0.770for the locus CMCTN86and TJ10, repectively. Values of genetic differentiation (Fst) and gene flow (Nm) ranged from 0.535 to 1.000 with an average of 0.776 and from 0.000 to 0.218 with a mean value of 0.072, respectively. Broad genetic base was found among the muskmelon germplasm used in this study. The average pair-wise Nei’s genetic distance value was 0.605. The highest genetic dissimilarity coefficient (GD=2.300) was between the germplasm AHM-241 and IAH-102, whereas germplasm IAH-251 and IAH-259 comprised lowest genetic diversity (GD =0.000). In the UPGMA dendrogram, among 96 germplasm of muskmelon 84 grouped in cluster “I” and other 12 in cluster “II”.

Keywords: Muskmelon, Microsatellite (SSR) marker, Polymorphism, Genetic diversity, Germplasm

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APA Molla, M. R., Ahmed, I., Rahman, S., Hossain, M. A., Salam, M. A., Chowdhury, M. A. Z., & Rohman, M. M. (2017). Genetic diversity among muskmelon (Cucumis melo L.) germplasm in Bangladesh as revealed by microsatellite markers. African Journal of Agricultural Research, 12(44), 3203-3213.
Chicago Md. Rezwan Molla, Iftekhar Ahmed, Sajia Rahman, Md. Amjad Hossain, Md. Abdus Salam, Md. Aziz Zilani Chowdhury and Md. Motiar Rohman. "Genetic diversity among muskmelon (Cucumis melo L.) germplasm in Bangladesh as revealed by microsatellite markers." African Journal of Agricultural Research 12, no. 44 (2017): 3203-3213.
MLA Md. Rezwan Molla, et al. "Genetic diversity among muskmelon (Cucumis melo L.) germplasm in Bangladesh as revealed by microsatellite markers." African Journal of Agricultural Research 12.44 (2017): 3203-3213.
   
DOI 10.5897/AJAR2017.12617
URL http://academicjournals.org/journal/AJAR/article-abstract/B17770966586

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