African Journal of Agricultural Research
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Article Number - CAC3C9537966


Vol.8(36), pp. 4565-4572 , September 2013
DOI: 10.5897/AJAR2013.7021
ISSN: 1991-637X



Full Length Research Paper

Tests confirm suitability of Ugandan soils for commercial growing of Artemisia annua Linn


Byamugisha Tarsisius1, Byamukama Robert2 and Ntale Muhammad2*




1National Drug Authority, Uganda.

2Department of Chemistry, School of Physical Sciences, College of Natural Sciences, Makerere University, Uganda


Email: muhntale@yahoo.co.uk






 Accepted: 30 August 2013  Published: 19 September 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The content of artemisinin from Artemisia annua Linn plants grown in different geographical regions of Uganda was investigated. Two analytical methods; high performance liquid chromatography (HPLC) and an ultraviolet (UV) spectrophotometric analytical procedure were used and the results were compared. The HPLC method was modified and revalidated before it was used for analysis of the plant samples. The spectrophotometric procedure was used without modification. Seven districts including Kapchorwa, Kabale, Kaberamaido, Kabarole, Wakiso, Rakai and Rukungiri were selected for the study. With both methods, higher content of artemisinin was obtained in plant samples from Kapchorwa (1.2%, w/w, HPLC) and Kabale (1.4%, w/w, HPLC) than in samples from Kaberamaido (0.47%, w/w, HPLC) and Wakiso (0.36%, w/w, HPLC). In the other districts, the content varied between 0.57 and 0.93%, w/w. Fortunately, even the low content of artemisinin that was observed in Kaberamaido and Wakiso compared very well with values reported in other parts of the world where the plant is widely grown for commercial and for experimental purposes. This suggested that Ugandan soils are adequately suitable for commercial production of A. annua.

 

Key words: Artemisia annua, content of artemisinin, geographical region, Uganda, high performance liquid chromatography (HPLC), ultraviolet (UV) spectroscopy.


APA (2013). Tests confirm suitability of Ugandan soils for commercial growing of Artemisia annua Linn. African Journal of Agricultural Research, 8(36), 4565-4572.
Chicago Byamugisha Tarsisius, Byamukama Robert and Ntale Muhammad. "Tests confirm suitability of Ugandan soils for commercial growing of Artemisia annua Linn." African Journal of Agricultural Research 8, no. 36 (2013): 4565-4572.
MLA Byamugisha Tarsisius, Byamukama Robert and Ntale Muhammad. "Tests confirm suitability of Ugandan soils for commercial growing of Artemisia annua Linn." African Journal of Agricultural Research 8.36 (2013): 4565-4572.
   
DOI 10.5897/AJAR2013.7021
URL http://academicjournals.org/journal/AJAR/article-abstract/CAC3C9537966

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