Full Length Research Paper
Ruzanna Ab Kadir1, Shahrul Hisham Zainal Ariffin1*, Rohaya Megat Abdul Wahab2, Sahidan Senafi1 and Fahrul Zaman Huyop3
1School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia.
2Department of Orthodontics, Faculty Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
3Department of Industrial Biotechnology, Faculty of Biosciences and Bioengineering, University Technology
Malaysia, Johor, Malaysia.
Stem cells are known to have the ability to renew themselves and differentiate into a diverse range of specialized cell types. Currently, the differentiation potential of human peripheral blood mononucleated cells in suspension as stem cells is not well-understood. The aim of this study was to investigate the differentiation potential of suspension mononucleated cells from human peripheral blood to differentiate. The osteoblast and osteoclast differentiation potential of suspension peripheral blood mononucleated cells were examined by molecular, biochemical and cell morphology analyses. The expression of osteoblast marker (osteonectin, SPARC) and osteoclast marker (tartrate resistant acid phosphatase, TRAP) as well as high alkaline phosphate (ALP) and TRAP enzyme activity were observed at days 14 and 10 of osteoblast and osteoclast differentiation, respectively. Morphology analyses showed that mononucleated cells successfully differentiated into osteoblasts and osteoclasts. The existence of stem cells in mononucleated cells was evaluated by the expression of a stem cell factor (KIT) and a haematopoietic stem cell marker (signalling lymphocytic activation molecule family member 1, SLAMF1). This study has shown that these suspension mononucleated cells possess differentiation potential through in vitro study. Human peripheral blood suspension mononucleated cells that have multi-lineage differentiation potential may provide a new source of stem cells that may be used for bone regeneration and tissue engineering applications.
Key words: Peripheral blood, osteoblast cells, osteoclast cells, osteoblast differentiation, osteoclast differentiation.
|APA||(2011). Differentiation potential of human suspension mononucleated peripheral blood cells. African Journal of Biotechnology , 10(52), 10756-10764.|
|Chicago||Ruzanna Ab Kadir, Shahrul Hisham Zainal Ariffin, Rohaya Megat Abdul Wahab, Sahidan Senafi and Fahrul Zaman Huyop. "Differentiation potential of human suspension mononucleated peripheral blood cells." African Journal of Biotechnology 10, no. 52 (2011): 10756-10764.|
|MLA||Ruzanna Ab Kadir, et al. "Differentiation potential of human suspension mononucleated peripheral blood cells." African Journal of Biotechnology 10.52 (2011): 10756-10764.|