African Journal of Biotechnology
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Article Number - 4ACED0A26333


Vol.10(15), pp. 2987-2990 , April 2011
DOI: 10.5897/AJB10.775
ISSN: 1684-5315



Full Length Research Paper

Prevalence of Candida species in the oral cavity of patients with periodentitis


Batool Sadeghi Nejad1,2*, Ablollah Rafiei1 and Fereshteh Moosanejad2




1Department of Mycoparasitology, Infectious Disease and Tropical Medicine Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2Department of Mycoparasitology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.


Email: batsad4@yahoo.com






 Accepted: 15 March 2011  Published: 11 April 2011

Copyright © 2011 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


During the past two decades, there has been a significant increase in the prevalence of fungal infections caused by Candida species. Oral candidiasis is a common opportunistic infection of the oral cavity caused by yeast fungi of the genus Candida on the mucous membranes of the mouth. To isolate and determine the incidence rate of oral Candida species in periodentitis and gingivitis patients referred to school of dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, this study was carried out in 172 patients with periodentitis and gingivitis aged 11 to 72 years. Swabs samples were taken from salivary secretion, the palate mucosa and dentine carious lesions and were cultured directly on Sabouraud dextrose agar medium. Isolated yeasts were identified by CHROMagarCandida, germ tube test and Clamidoconidia formation (corn meal agar plus Tween 80 medium). Results showed the prevalence of Candida albicans (n = 120, 75%),Candida glabrata (n = 20, 12.5%), Cadida tropicalis (n = 10, 6.5%), Candida dubliniensis (n = 6, 4.0%) and Cadida krusei (n = 3, 2.0%). In this investigation, germ tube-test and chlamydospore formation were positive in the isolates that produced dark-green colonies and were considered as C. dubliniensis and light-green colonies were identified as C. albicans. CHROMagar Candida is a satisfactory isolation medium for oral and dental specimens. It is a satisfactory method for correct and rapid identification of common yeast species and easy recognition of mixed cultures in clinical samples.

 

Key words: Periodentitis, gingivitis, oral candidiasis, CHROMagar


APA (2011). Prevalence of Candida species in the oral cavity of patients with periodentitis. African Journal of Biotechnology , 10(15), 2987-2990.
Chicago Batool Sadeghi Nejad, Ablollah Rafiei and Fereshteh Moosanejad. "Prevalence of Candida species in the oral cavity of patients with periodentitis." African Journal of Biotechnology 10, no. 15 (2011): 2987-2990.
MLA Batool Sadeghi Nejad, Ablollah Rafiei and Fereshteh Moosanejad. "Prevalence of Candida species in the oral cavity of patients with periodentitis." African Journal of Biotechnology 10.15 (2011): 2987-2990.
   
DOI 10.5897/AJB10.775
URL http://academicjournals.org/journal/AJB/article-abstract/4ACED0A26333

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