African Journal of Biotechnology
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Article Number - 56574AE56324

Vol.17(11), pp. 350-355 , March 2018
ISSN: 1684-5315

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Full Length Research Paper

Toxicity of powdered and ethanolic extracts of Uvaria chamae (Annonaceae) Bark on selected stored product insect pests

Negbenebor, H. E.
  • Negbenebor, H. E.
  • Department of Computing and Applied Science, Baze University, Abuja, Nigeria.
  • Google Scholar
Makanjuola, W. A
  • Makanjuola, W. A
  • Department of Zoology, University of Lagos, Nigeria.
  • Google Scholar
Denloye, A. A.
  • Denloye, A. A.
  • Department of Zoology and Environmental Biology, Lagos State University, Lagos, Nigeria.
  • Google Scholar
Nura, S.
  • Nura, S.
  • Department of Biology, Ahmadu Bello University, Zaria, Nigeria.
  • Google Scholar

 Received: 21 September 2017  Accepted: 10 January 2018  Published: 14 March 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

A study was carried out on the insecticidal effects of the powdered stem bark extract of Uvaria chamae and its ethanolic extract on three most devastating stored products pests (Coleopterous) in Nigeria, namely: Callosobruchus maculatus F. (Bruchidae), Rhizopertha dominica F. (Bostrichidae) and Sitophilus zeamais Motschulsky (Curculionidae). Graded concentrations of each formulation of the powdered bark and ethanolic extracts were in exposure chambers of each insect in laboratory bio-assays under ambient conditions (25±2°C). S. zeamais, R. dominica and C. maculatus were exposed to the following concentrations 0.00, 0.10, 0.20, 0.40, 0.80, and 1.60 mg/L grains in three replicates per treatment and control. The mortality of the insects was used to compute mean lethal concentration (LC50) values by probit analysis. All the concentrations tested showed appreciable toxicity against each test insect species. The computed LC50 values for powder formulation gave significantly (P≤0.05) higher toxicity against C. maculatus (1.281 g/kg) than either S. zeamais (2.145 g/kg) or R. dominica (5.189 g/kg). However, the ethanolic extract was more toxic on C. maculatus (0.134 mL/L), S. zeamais (0.173 mL/L) or R. dominica (0.359 mL/L). It was found that the higher the concentration of the ethanolic extract, the higher the mortality. The result implies that, U. chamae powdered and ethanolic stem bark extracts have potentials for use during storage of grains, ensuring food security, profit maximization and availability of seeds for the next planting season without being damaged by these test insect species. The presence of high concentration of steroids and terpenes may be responsible for the observed high insecticidal activity of the test extracts.

Key words: Bioassay, Callosobrochus maculatus, storage insect, Uvaria chamae.

AOAC (2000). Official Methods of Analysis. International Association of Official Analytical Chemists.17th edition. Washington D.C.


Abbott WS (1925). A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18:265-267.


Adebowale KO, Adedire CO (2006). Chemical composition and insecticidal properties of the underutilized Jatropha curcas seed oil. Afr. J. Biotechnol. 5(10):901-906.


Ahmed DA (2013). Post harvest losses; making Nigerians farmers poorer 21 November 2013, Hits 530 post-harvest losses. The Daily Trust. 21st November, 2013.


Babatunde R, Oyatoye E (2005). Food Security and Marketing Problems in Nigeria: The Case of Maize Marketing in Kwara State. Proceedings of the International Conference on Research for Development in Agricultural Forestry, Food and Natural Resources Management, Stuttgart-Hohenheim, Germany, 11–13 Oct. 2005.


Benner JP (1993). Pesticide compounds from higher plants. Pestic. Sci. 39:95-102.


Bogunjoko JST (1987). Storage problems of Nigeria farmers. Longman Publishers. pp. 6-12.


Denloye AA, Makanjuola WA, Don-Pedro KN, Negbenebor HE (2007). Insecticidal effects of Tephrosia vogelii Hook (Leguminosae) leaf powder and extracts on Sitophilus zeamais Motsch, Callosobruchus maculatus F. and Tribolium casteneum Herbst. Niger. J. Entomol. 24:94-97.


FAO (2011). Global food losses and food waste. Food and Agriculture Organization of the United Nations. Study conducted for the International Congress SAVE FOOD at Interpack 2011 Düsseldorf, Germany, pp. 1-23.


Finney DJ (1971). Statistical Method in Biological Assay. 2nd ed. London: Griffin; 668 p.


Idachaba FS (2004). Food security in Nigeria: Challenges under democratic dispensation, 9th ARMTI Annual Lecture, Ilorin, Nigeria. 24/3/2004.


Ihimodu II (2004). Marketing of agricultural products and the Food Security Programme in Nigeria. Paper presented at the 13th Annual Congress of the Nigeria Rural Sociological Association at LAUTECH, Ogbomosho, Nigeria.


IITA (2001). COWPEA: Post-Harvest Operations. International Institute of Tropical Agriculture, Annual Report. Ibadan, Nigeria. 11p.


IITA (2009). Cereal Crops: Rice, Maize, Millet, Sorghum, Wheat International Institute of Tropical Agriculture. Research for Development; Cereals and Legumes. Maize (Zea mays). Ibadan, Nigeria. pp. 1-31.


Kone ML, Ouattara KL, Gnahoue GL, Ouattara A, Coulibaly A (2013). Ethnopharmacological and phytochemical screening of some plants involved in the treatment of abdominal infections in the Department of Kouto (Cote D'Ivoire). Scholars J. Appl. Med. Sci. 1(2):56-61.


Lajide L, Adedire CO, Muse WA, Agele SO (1998). Insecticidal activity of powders of some Nigerian plants against maize weevil. In: Lale, N.E.S., Molta, N.B., Donli, P.O., Duke, M.C., and Aminu Kano, M. (eds.), Entomology in the Nigerian Economy. Entomology Society of Nigeria Occasional Publication. 31:227-235.


Lale NES, Mustapha A (1999). Potential of combining neem seed oil with varietal resistance for the management of the cowpea bruchid Callosobruchus maculatus in Nigeria. J. Stored Prod. Res. 35:135-143.


Lowenberg-Deboer J, Ibro G (2008). The potential effect of economic growth and technological innovation on women's role in the cowpea value chain in Kano State, Nigeria. 158 p.


Meena MS, Ashwani K, Singh KM, Meena HR (2009). Farmers' attitude towards post-harvest issues of horticultural crops. Indian Res. J. Ext. Educ. 9(3):15-19.


Obi VI, Onuoha C (2000). Extraction and characterization methods plants and plant products. In. Biological and agricultural technique, Ogbulie J.N., and Ojiako, O.A. (eds.), Websmedia Publishers, Owerri. pp. 271-286.


Ogueke CC, Ogbulie JN, Njoku HO (2006). Antimicrobial properties and preliminary phytochemical analysis of ethanolic extracts of Alstonia bonnie. Niger. J. Microbiol. 20(2):896-899.


Okon JE, Udosen IR, Mbong EO (2013). Phytochemical screening and effect of ethanolic root extract of Uvaria chamae on heamatological parameters on albino rats in Akwa Ibom State, Nigeria. Merit Res. J. Environ. Sci. Toxicol. 1(12):16-20.


Oluremi BB, Osungunna MO, Omafuma OO (2010). Comparative assessment of antibacterial activity of Uvaria chamae parts. Afr. J. Microbiol. Res. 4(13):1391-1394.


Osoagwu GGE, Ihenwosu AO (2014). Phytochemical composition and antimicrobial activity of the leaves of Alchornea cordifilia, Sanseviera liberica, and Uvaria chamae. Clin. Ther. 2(1):001-012.


Patrick I (2013). Nigeria records over 40 percent post-harvest losses sustainable food security in Nigeria. SUFOS, 19 Nov. 2013.


Pereira PAA, del Peloso MJ, Lacosta JGC, Yokoyama LP (2001). Beans product perspective for production, consumption and genetic improvement. Paper presented at the Cowpea Research National Meeting, Embrapa, Trersina, PIAU, Brazil.


Samuel A, Saburi A, Usanga OE, Ikotun I, Isong IU (2011). Post–harvest food losses reduction in maize production in Nigeria. Afr. J. Agric. Res. 6(21):4833-4839.


Sousa AH, de Maracaja PB, Silva RM, Moura MN, Andrade WG (2005). Bioactivity of vegetal powders against Callosobruchus maculatus (Coleoptera: Bruchidae) in cowpea bean and seed physiological analysis. Revista de Biological E Ciencias Da Terra 5(2):19-23.


Weinzierl RA (2000). Botanical insecticides, soaps, and oils. In. Rechcigl, J.E., and Rechcigl, N.A. (eds). Biological and biotechnological control of insect pests. CRC Press, Boca Raton, Florida. pp. 101-118.



APA Negbenebor, H. E., Makanjuola, W. A., Denloye, A. A., & Nura, S. (2018). Toxicity of powdered and ethanolic extracts of Uvaria chamae (Annonaceae) Bark on selected stored product insect pests. African Journal of Biotechnology , 17(11), 350-355.
Chicago Negbenebor, H. E., Makanjuola, W. A., Denloye, A. A. and Nura, S.. "Toxicity of powdered and ethanolic extracts of Uvaria chamae (Annonaceae) Bark on selected stored product insect pests." African Journal of Biotechnology 17, no. 11 (2018): 350-355.
MLA Negbenebor, et al. "Toxicity of powdered and ethanolic extracts of Uvaria chamae (Annonaceae) Bark on selected stored product insect pests." African Journal of Biotechnology 17.11 (2018): 350-355.

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