African Journal of Biotechnology
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Article Number - 670A81264285

Vol.16(20), pp. 1168-1175 , May 2017
ISSN: 1684-5315

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Full Length Research Paper

Exploration of Sri Lankan soil fungi for biocontrol properties

Herath H. H. M. A. U.
  • Herath H. H. M. A. U.
  • Department of Plant Sciences, Faculty of Science, University of Colombo, Sri Lanka.
  • Google Scholar
Wijesundera R. L. C.
  • Wijesundera R. L. C.
  • Department of Plant Sciences, Faculty of Science, University of Colombo, Sri Lanka.
  • Google Scholar
Chandrasekharan N. V.
  • Chandrasekharan N. V.
  • Department of Chemistry, Faculty of Science, University of Colombo, Sri Lanka.
  • Google Scholar
Wijesundera W. S. S.
  • Wijesundera W. S. S.
  • Department of Molecular Biology and Biochemistry, Faculty of Medicine, University of Colombo, Sri Lanka.
  • Google Scholar

 Received: 20 January 2017  Accepted: 29 March 2017  Published: 17 May 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

Sri Lankan soil is a rich source of fungi with new strains that are not well explored to date. In the present study, a total of 83 soil fungi were isolated from different locations of Sri Lanka using chitin and β-1,3-glucan selective media. Of the 83 isolates, 22 isolates showed high biocontrol activities against seven selected plant pathogenic fungi in dual culture plate assay. These 22 isolates were further analyzed for chitinase, glucanase production and for antifungal activities. Trichoderma sp. 1 showed significantly high mean chitinase (0.60±0.012 nkat/ml) and glucanase (0.42±0.031 nkat/ml) activities after 24 h and 96 h of incubation respectively compared to other isolates. Furthermore, it revealed the highest mean percentage (76.66±7.6) of inhibition against Phytophthora meadii in the well diffusion method using chitinase filtrates. Penicillium sp. 1 showed the highest mean percentage (64.75±1.6) of inhibition against Rigidoporus microporus in well diffusion method with glucanase filtrates. Three Trichoderma isolates that unveiled high enzyme activities as well as high antifungal activities were identified as Trichoderma erinaceum (Trichoderma sp. 1), Trichoderma   virens (Trichoderma sp. 5) and Trichoderma asperellum (Trichoderma sp. 8) using molecular characterization.


Key words: Chitin, glucan, antifungal, Trichoderma, Phytophthora, Rigidoporus.

Ahmad KM, Ahmad M, Javed, S (2010). Optimization of culture media for enhanced chitinase production from a novel strain of Stenotrophomonas maltophilia using response surface methodology. J. Microbiol. Biotechnol. 20(11):1597-1602.


Bell DK, Well HD, Markham CR (1982). In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology 72:379-382.


Chakraborty BN, Chakraborty U, Saha A, Dey PL, Sunar K (2010). Molecular Characterization of Trichoderma viride and Trichoderma harzianum Isolated from Soils of North Bengal Based on rDNA Markers and Analysis of Their PCR-RAPD Profiles. Glob. J. Biotechnol. Biochem. 5(1):55-61.


Coomaraswamy U, Fonseka RN (1981). A Handbook to the Soil Fungi of Sri Lanka. National Science Council of Sri Lanka, Colombo. P 7.


Elad Y, Kapat A (1999). The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea. Eur. J. Plant Pathol. 105:177-189.


El-Katatny MH, Somitsch W, Robra KH, El-Katatny MS, Gübitz GM (2000). Production of chitinase and β-1, 3-glucanase by Trichoderma harzianum for control of the phytopathogenic fungus Sclerotium rolfsii. Food Technol Biotechnol. 38(3):173-180.


Jayalakshmi SK, Raju S, Usha R, Benagi VI, Sreeramula K (2009). Trichoderma harzianum L1 as a potential source for lytic enzymes and elicitor of defense responses in chickpea (Cicer arietinum) against wilt disease caused by Fusarium oxysporum f.sp. ciceri. Aust. J. Crop Sci. 3(1):44-45.


John RP, Tyagi RD, Prévost D, Brar SK, Pouleur S, Surampalli RY (2010). Mycoparasitic Trichoderma viride as a biocontrol agent against Fusarium oxysporum f. sp. adzuki and Pythium arrhenomanes and as a growth promoter of soybean. Crop Prot. 29:1452-1459.


Karunarathna SC, Udayanga D, Maharachchikumbura SN, Pilkington M, Manamgoda DS, Wijayawardene DNN, Ariyawansa HA, Bandara AR, Chukeatirote E, McKenzie EHC, Hyde KD (2012). Current status of knowledge of Sri Lankan mycota. Curr. Res. Environ. Appl. Mycol. 2(1):18-29.


Lorito M, Woo SL, Garcia I, Colucci G, Harman GE, Pintor-Toro JA, Filippone E, Muccifora S, Lawrence CB, Zoina A, Tuzun S, Scala F (1998). Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens. Proc. Natl. Acad. Sci. USA 95(14):7860-7865.


Lorito MCK, Hayes A, Di Pietro SL, Woo Harman GE (1994). Purification, characterization and synergistic activity of a glucan 1,3-ß-glucosidase and an N-actyl-ß-glucosaminidase from Trichoderma harzianum. Phytopathology 84:398-405.


Matroudi S, Zamani MR, Motallebi M (2009). Antagonistic effects of three species of Trichoderma sp. on Sclerotinia sclerotiorum, the causal agent of canola stem rot. Egypt. J Biol. 11:37-44.


Muhammad S, Amusa NA (2003). In-vitro inhibition of growth of some seedling blight inducing by compost-inhabiting microbes. Afr. J. Biotechnol. 2(6):161-164.


Ramezani H (2009). Efficacy of fungal and bacterial bioagents against Fusarium oxysporum f.sp. ciceri on chickpea. Plant Prot. J. 1:108-113.


Rifai MA (1969). A revision of the genus Trichoderma. Mycol. Paper 116: 1-56.


Severgnini MES (2006).Isolation and characterization of two chitinase and one novel glucanase genes for engineer plant defence against fungal pathogens. Ph.D. Thesis, Murdoch University, Western Australia.


Shabir R, Rubina L, Ebenezer JK, Zaffar AB (2012). Comparative efficacy of Trichoderma viride, T. harzianum and carbendazim against damping-off disease of cauliflower caused by Rhizoctonia solani Kuehn. J. Biopestic. 5(1):23-27.


Sharaf EF, El-Sarrany AEAQ, El-Deeb M (2012). Biorecycling of shrimp shell by Trichoderma viride for production of antifungal chitinase. Afr. J. Microbiol. Res. 6(21):4538-4545.


Sharma P, Sharma M, Srivastava M (2014). Heterologous expression and characterization of purified partial endochitinase (ech-42) isolated from Trichoderma harzianum. Afr. J. Biotechnol. 13(21):2159-2165.


Zechner-Krpan V, Petravic-Tominac V, Gospodaric I, Saji L, Dakovic S, Filipovic-Grcic J (2010). Characterization of β-glucans isolated from brewer's yeast and dried by different methods. Food Technol. Biotechnol. 48(2):189-197.



APA Herath, H. H. M. A. U., Wijesundera, R. L. C., Chandrasekharan, N. V., & Wijesundera, W. S. S. (2017). Exploration of Sri Lankan soil fungi for biocontrol properties. African Journal of Biotechnology , 16(20), 1168-1175.
Chicago Herath H. H. M. A. U., Wijesundera R. L. C., Chandrasekharan N. V. and Wijesundera W. S. S.. "Exploration of Sri Lankan soil fungi for biocontrol properties." African Journal of Biotechnology 16, no. 20 (2017): 1168-1175.
MLA Herath H. H. M. A. U., et al. "Exploration of Sri Lankan soil fungi for biocontrol properties." African Journal of Biotechnology 16.20 (2017): 1168-1175.

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