African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12049

Full Length Research Paper

Molecular diagnosis of phytoplasma transmission from zygotic embryos to in vitro regenerated plants of coconut palm (Cocos nucifera L.)

DARAMCOUM Wentoin Alimata Marie Pierre
  • DARAMCOUM Wentoin Alimata Marie Pierre
  • Université Félix Houphouët Boigny, UFR Biosciences, Laboratoire de Génétique, 22 BP 582 Abidjan 22, Côte d’Ivoire.
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KONAN Konan Jean-Louis
  • KONAN Konan Jean-Louis
  • Centre National de Recherche Agronomique (CNRA), Station Marc Delorme of Port-Bouët, 07 BP 13 Abidjan 07, Côte d’Ivoire.
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YAO Saraka Didier Martial
  • YAO Saraka Didier Martial
  • Université Peleforo Gon Coulibaly, UFR des Sciences Biologiques, BP 1328 Korhogo, Côte d’Ivoire.
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YAIMA Arocha Rosete
  • YAIMA Arocha Rosete
  • Sporometrics, 219 Dufferin Street, Suite 20C, Toronto, ON M6K 3J1, Canada.
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KOFFI Eric-Blanchard Zadjéhi
  • KOFFI Eric-Blanchard Zadjéhi
  • Centre National de Recherche Agronomique (CNRA), Station Marc Delorme of Port-Bouët, 07 BP 13 Abidjan 07, Côte d’Ivoire.
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YOBOUE Koffi
  • YOBOUE Koffi
  • Centre National de Recherche Agronomique (CNRA), Station Marc Delorme of Port-Bouët, 07 BP 13 Abidjan 07, Côte d’Ivoire.
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KOUASSI Kan Modeste
  • KOUASSI Kan Modeste
  • Centre National de Recherche Agronomique (CNRA), Station Marc Delorme of Port-Bouët, 07 BP 13 Abidjan 07, Côte d’Ivoire.
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KOUADJO Claude Ghislaine
  • KOUADJO Claude Ghislaine
  • Centre National de Recherche Agronomique (CNRA), Station Marc Delorme of Port-Bouët, 07 BP 13 Abidjan 07, Côte d’Ivoire.
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KOFFI Edmond
  • KOFFI Edmond
  • Centre National de Recherche Agronomique (CNRA), Station Marc Delorme of Port-Bouët, 07 BP 13 Abidjan 07, Côte d’Ivoire.
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KOFFI Kouadio Kan Ghislain
  • KOFFI Kouadio Kan Ghislain
  • Centre National de Recherche Agronomique (CNRA), Station Marc Delorme of Port-Bouët, 07 BP 13 Abidjan 07, Côte d’Ivoire.
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N’GUETTA Assanvo Simon-Pierre
  • N’GUETTA Assanvo Simon-Pierre
  • Université Félix Houphouët Boigny, UFR Biosciences, Laboratoire de Génétique, 22 BP 582 Abidjan 22, Côte d’Ivoire.
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  •  Received: 24 April 2018
  •  Accepted: 05 June 2018
  •  Published: 04 July 2018

Abstract

The aim of this study was to investigate the transmission of the lethal yellowing disease (LYD) of coconut tree caused by a phytoplasma from the zygotic embryo to the regenerated plantlet in vitro. From a total of 30 trees, 150 mature coconut nuts where harvested. These nuts were used to extract 150 zygotic embryos. From this package, 96 zygotic embryos were used to regenerate 96 young coconut seedlings in vitro and the 54 others were used to extract total DNA. From the stem of the 30 palms at the stage 1 of the LYD, phloem sample were also collected. From the regenerated in vitro-plantlets at 6 months age, leaf sample were collected. From the molecular diagnosis by PCR, 80% of the phloem samples carried the 16S rRNA gene of the phytoplasma responsible for LYD. All the zygotic embryos and in vitro-plantlets regenerated were healthy. So, coconut zygotic embryos can be used for the safe exchange of genetic material regarding lethal yellowing disease. The regenerated in vitro plantlet are free of disease.

Key words: Coconut, phytoplasma, transmission, in vitro.