African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 11997

Full Length Research Paper

Investigations of different strategies for high frequency regeneration of Dendrobium malones ‘Victory’

Saima Anjum, Muhammad Zia and M. Fayyaz Chaudhary*
Department of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Email: [email protected]

  •  Accepted: 11 November 2005
  •  Published: 02 October 2006

Abstract

Regeneration of orchid, Dendrobium malones “Victory”, has been established by direct differentiation and through callus formation. With direct thin leaf section differentiation method, the number of protocorm like bodies (PLBs) formed from Thin Section (TS) explants obtained from a single leaf was 10 times more than that from a single whole leaf, in a short time period. This therefore suggested that the proliferation potential is distributed all along the seedling leaves and morphogenically competent cells were not limited in the basal ends or the tips of the leaves in TS of orchid leaf cultures. Full MS media supplemented with auxins/cytokinins either in single or in combination along with peptone, yeast extract, casein hydrolysate (each at 100 mg/l), banana powder (40 g/l), 15% coconut water and 2% sucrose have shown excellent results for high frequency regeneration in orchid cultures. Like many other higher plants, regeneration of D. malones leaves responded by developing proliferative loci in some “Predetermined Regenerative” cells in the dermal layers by tissue culture methods.

 

Key words: Dendrobium malones, protocorm, callus, organogenesis.