African Journal of Biotechnology
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Article Number - 9D2B3E07910


Vol.8(7), pp. 1176-1182 , April 2009
DOI: 10.5897/AJB09.224
ISSN: 1684-5315



Full Length Research Paper

Evaluation of the antimicrobial properties of unripe banana (Musa sapientum L.), lemon grass (Cymbopogon citratus S.) and turmeric (Curcuma longa L.) on pathogens


Fagbemi, Josephine Ferdinand1, Ugoji, Esther1*, Adenipekun, Tayo2 and Adelowotan, Omotoyin2




 

1Department of Botany and Microbiology, University of Lagos, Lagos, Nigeria.

2Department of Bacteriology and Parasitology, College of Medicine, Lagos, Nigeria.


Email: ugojie@mail.com






 Accepted: 25 February 2009  Published: 06 April 2009

Copyright © 2009 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

The investigation on the potency of unripe banana (Musa sapientum L.), lemon grass (Cymbopogon citratus S.) and turmeric (Curcuma longa L.) was carried out against pathogens. The formulations were in the powder form as used locally. The antimicrobial activity of these plants was examined using different solvents and efficacy was compared. The solvents were ethanol (70%, v/v) and water. Antimicrobial activity was carried out by the agar well diffusion method. The clinical isolates include aerobic, facultative bacteria namely: Stapyhlococcus aureus ATCC 25921, S. aureus, Salmonella paratyphi, Shigella flexnerii, Escherichia coli ATCC 25922, E. coli, Klebsiella pneumoniae, Bacillus subtilis and Pseudomonas aeruginosa. Crude extracts of the solvents varied in zones of inhibition. All the Gram-positive bacteria (S. aureusS. aureus ATCC 25921 and B. subtilis) and all Gram-negative bacteria (E. coli ATCC 25922, E. coli, P. aeruginosa, S. paratyphi,S. flexneri and K. pneumonia) were susceptible to ethanolic extracts of unripe banana, lemon grass and turmeric while some namely E. coli ATCC 25922, E. coli,P. aeruginosa and S. flexneri were not susceptible to aqueous extracts of the three medicinal plants. The minimum inhibition concentration (MIC) ranged from 4 – 512 mg/ml while the minimum bactericidal concentration (MBC) ranged from 32 – 512 mg/ml depending on isolates and extracting solvent. Ethanolic extracts showed greater antimicrobial activity than aqueous extracts. The killing rate of the extracts varied. Unripe banana had less than 2 h killing time for S. aureus ATCC 25921, turmeric less than 3 h for E. coli while lemon grass had more than 3 h killing time for S. paratyphi.

 

Key words: Antimicrobial activity, unripe banana, lemon grass, turmeric, death rate.

 

 


APA Fagbemi, J. F., Ugoji, E., Adenipekun, T., & Adelowotan, O. (2009). Evaluation of the antimicrobial properties of unripe banana (Musa sapientum L.), lemon grass (Cymbopogon citratus S.) and turmeric (Curcuma longa L.) on pathogens. African Journal of Biotechnology , 8(7), 1176-1182.
Chicago Fagbemi, Josephine Ferdinand, Ugoji, Esther, Adenipekun, Tayo and Adelowotan, Omotoyin. "Evaluation of the antimicrobial properties of unripe banana (Musa sapientum L.), lemon grass (Cymbopogon citratus S.) and turmeric (Curcuma longa L.) on pathogens." African Journal of Biotechnology 8, no. 7 (2009): 1176-1182.
MLA Fagbemi, et al. "Evaluation of the antimicrobial properties of unripe banana (Musa sapientum L.), lemon grass (Cymbopogon citratus S.) and turmeric (Curcuma longa L.) on pathogens." African Journal of Biotechnology 8.7 (2009): 1176-1182.
   
DOI 10.5897/AJB09.224
URL http://academicjournals.org/journal/AJB/article-abstract/9D2B3E07910

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