African Journal of Biotechnology
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Article Number - 9F56ED264279


Vol.16(20), pp. 1159-1167 , May 2017
DOI: 10.5897/AJB2016.15783
ISSN: 1684-5315



Full Length Research Paper

Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province



Depison
  • Depison
  • Faculty of Animal Husbandry, Jambi University, Indonesia.
  • Google Scholar
Sarbaini Anwar
  • Sarbaini Anwar
  • Faculty of Animal Husbandry, Andalas University, Indonesia.
  • Google Scholar
Jamsari
  • Jamsari
  • Faculty of Agriculture, Andalas University, Indonesia.
  • Google Scholar
Arnim
  • Arnim
  • Faculty of Animal Husbandry, Andalas University, Indonesia.
  • Google Scholar
Yurnalis
  • Yurnalis
  • Faculty of Animal Husbandry, Andalas University, Indonesia.
  • Google Scholar







 Received: 15 November 2016  Accepted: 24 April 2017  Published: 17 May 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The objective of this research was to obtain quantitative growth hormone gene polymorphism association between growth hormone gene genotype with quantitative characteristics of the thin-tail sheep in the highlands and lowlands of Jambi Province. Two phases of research were done on the field and in the laboratory. Field research which was conducted to obtain the quantitative characteristics data includes; withers height (WH), body length (BL), chest grid (ChG), chest depth (ChD), chest width (ChW), body weight (BW) and body weight gain (BWG). Laboratory research included: DNA isolation, amplification and gel purification, characterization and identification using PCR-RFLP with the MspI, and AluI. Quantitative characteristics data was collected from 240 heads of thin-tailed sheep. Blood samples were collected from 160 of them and all were between the ages of 1 and 2 years (I1 = pair of permanent teeth). Field research was conducted in the Kerinci District and Sungai Penuh City (Highlands) and Muara Jambi Districts and Batanghari Districts (lowlands). The purposive sampling technique used in this research revealed that: 1) quantitative characteristics (BW, BWG, BL, ChG, ChD, and ChW) of thin-tailed sheep both male and female in highland were better than in the lowlands; 2) studied locus was polymorphic on the highlands and lowlands; 3) the highest of genotype and allele frequencies of both highlands and lowlands were related to the genotype (+/+) and allele (+), respectively; 4) the highest quantitative characteristic (BW BW, BWG, BL, ChG, ChD, and ChW) of thin-tailed sheep was genotype (+/+); and 5) the diversity of GH genes MspI and AluI were associated with BW, BWG, BL, ChG, ChD, and ChW of thin-tailed sheep both on the highlands and lowlands of Jambi Province.

 

Key words: Characterization, local sheep, thin-tailed sheep, diversity, growth hormone (GH) gene, lowlands, highlands, PCR-RFLP.

Ahmaed Z, Babar ME, Hussain T, Nadeem A, Awan FI, Wajid A, Shah SA, Ali MM (2014). Genetic diversity analysis of kail sheep by using microsatellite markers. J. Anim. Plant Sci. 24(5):1329-1333.

 

Alakilli YM, Mahrous KF, Salem LM, Ahmed ES (2012). Genetic polymorphism of five genes associated with growth traits in goat. Afr. J. Biotechnol. 11(82):14738-14748.

 
 

Beauchemin VR, Thomas MG, Franke DE, Silver GA (2006). Evaluation of DNA polymorphisms involving growth hormone relative to growth and carcass characteristics in Brahman steers. Genet. Mol. Res. 5(3):438-447.

 
 

Botstein D, White RL, Skolnick M, Davis RW (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J. Hum. Genet. 32(3):314-331.

 
 

Calderon A, Armstrong DV, Ray DE, Denise SK, Enns RM, Howison CM (2005). Productive and reproductive response of Holstein and Brown Swiss heat stressed dairy cows to two different cooling systems. J. Anim. Vet. 4(6):572-578.

 
 

Crawford AM, Dodds KG, Ede AJ, Piersen CA, Momtgomery GW, Garmonsway HG, Beattie AE, Davis K, Maddox JF, Kappes SW, Stone RT, Ngyen TC, Penty JM, Lord EA, Broom JE, Buitkamp J, Schwaiger W, Epplen JT, Mathew P, Hulme DJ, Beh KJ, McGraw RA, Beattie CW (1995). An autosomal genetic linkage map of the sheep genome. Genetics 140(2):703-724.

 
 

Department of Animal Husbandry and Animal Health Jambi Provinve (2014). Animal Husbandry and Health Statistics Jambi Province, Jambi.

 
 

Di Stasio L, Destefanis G, Brugiapaglia A, Albera A, Rolando A (2005). Polymorphism of the GHR gene in cattle and relationships with meat production and quality. Anim. Genet. 36(2):138-140.
Crossref

 
 

Elkorshy N, Karima F, Mahrous, Salem LM (2013). Genetic Polymorphism Detection in Four Genes in Egyptian and Saudi Sheep Breeds. World Appl. Sci. J. 27(1):33-43

 
 

Gasper V (2006). Teknik Analisis dalam Penelitian Percobaan. Penerbit. Tarsito Bandung.

 
 

Ge W, Davis ME, Hines HC, Irvin KM, Simmen RCM (2003). Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with blood serum insulin-like growth factor I concentration and growth traits in Angus cattle. J. Anim. Sci. 81(3):641-648.
Crossref

 
 

Ghazi KSA, Moradi-Shahrbabak H, Sadeghi M, Faraji R, Mohammadabadi MR (2014). Study polymorphism of ovine IGFBP-3 Gene and its association with carcass traits and fattyacids profiles in Zel sheep using PCR-SSCP. J. Agric. Biotechnol. 5(4):98-110.

 
 

Hajihosseinlo A, Semsarnejad A, Abollow E, Hashrafi F, Negahdary M (2013). Effect of GH gene polymorphismson biometric traits in Makooei sheep. Ann. Biol. Res. 4(6):351-355.

 
 

Hartl DL, Clark AG (1997). Principles of Population Genetic. Edth. Sunderland, Massachusetts: Sinauer Asociateea Inc.

 
 

Honarvar M, Moradi-Shahrebabak H, Sadeghi M, Behzadi SH, Mohamadabadi MR (2012). Study of the polymorphism at the 5' flanking region of the ovine IGF-I Gene and its association with carcass traits in Zel (tailed) and Lori-Bakhtiari (fat-tailed) breed sheep using PCR-SSCP. J. Agric. Biotechnol. 4(2):71-82.

 
 

https://www.ncbi.nlm.nih.gov/probe/docs/. Diakses 9 Januari 2015

 
 

Hua GH, Chen SL, Yu JN, Cai KL, Wu CJ, Li QL, Zhang CY, Liang AX, Han L, Geng LY, Shen Z, Xu DQ, Yang LG (2009). Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Sci. 81(2):391-395.
Crossref

 
 

Idris A, Kijora C, El-Hag FM, Salih AM, Elmola SAF (2014). Climate change adaptation strategies for sheep production in range land of Kordofan Region. World Essays J. 1(1):20-25.

 
 

Jakaria (2008). Keragaman genetik gen hormon pertumbuhan pada sapi pesisir Sumatera Barat. Disertasi. Sekolah Pascasarjana, Institut Pertanian Bogor, Bogor.

 
 

Javanmard A, Mohammadabadi MR, Zarrigabayi GE, Gharahedaghi AA, Nassiry MR, Javadmansh A, Asadzadeh N (2008). Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bos taurus). Russ. J. Genet. 44(4):495-497.
Crossref

 
 

Javanmard A, Nader A, Mohammad B, Javad T (2005). The allele and genotype frequencies of bovine pituitary spesific transcription factor and leptin genes in Iranian cattle and buffalo population using PCR-RFLP. Iran. J. Biotechnol. 3(2):104-108.

 
 

Jia JL, Zhang LP, Wu JP, Ha ZJ, Li WW (2014). Study of the correlation between GH gene polymorphism and growth traits in sheep. Genet. Mol. Res. 13(3):7190-7200.
Crossref

 
 

Khodabakhshzadeh R, Mohamadabadi MR, Esmailizadeh AK, Moradi-Shahrebabak H, Bordbar F, Ansari NS (2016a). Identification of available mutations in the first-half (from 5' end) of exon 2 of GDF9 gene in crossbred sheep from crossing of Romanov and Lori-Bakhtiari breeds. Anim. Prod. Res. 4(4):15-26.

 
 

Khodabakhshzadeh R, Mohammadabadi MR, Esmailizadeh AK, Moradi-Shahrebabak H, Bordbar F, Ansari NS (2016b). Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Pol. J. Vet. Sci. 19(2):281-289.
Crossref

 
 

Kumari R, Kumar R, Meena AS, Jyotsana B, Prince LLL, Kumar S (2014). Genetic polymorphism of growth hormone gene in native sheep breeds of india. Indian J. Small Rumin. 20(2):15-18.

 
 

Li WH, Graur D (1991) Fundamentals of Molecular Evolution. Sinauer Associates. Sunderland, Massachusetts.

 
 

Machado MA, Schuster I, Martines ML, Campos AL (2003). Genetic diversity of four breed using microsatellite markers. Rev. Bras. Zool. 32(1):93-98.
Crossref

 
 

Mahrous KF, Sabry NM, Altwaty NH, Salem LM (2014). Molecular comparative study of growth hormone receptor (GHR) gene in Egyptian and Saudi breeds of sheep (Ovis aries). Int. J. Adv. Res. 2(10):1102-1111.

 
 

Malewa AD, Hakim L, Maylinda S, Husain MH (2014). Growth hormone gene polymorphisms of Indonesia fat tailed sheep using PCR-RFLP and their relationship with growth traits. Livest. Res. Rural Dev. 26(6):115.

 
 

Mohammadabadi MR (2012). Relationships of IGFBP-3 gene polymorphism with cashmere traits in raini cashmere goat. Mod. Genet. J. 7(2):115-120.

 
 

Mohammadabadi MR, Sattayimokhtari R (2013). Estimation of (co) variance components of ewe productivity traits in kermani sheep. Slovak J. Anim. Sci. 46(2):45-51.

 
 

Mohammadabadi MR, Torabi A, Tahmourespoor M, Baghizadeh A, Mohammadi A (2010). Analysis of bovine growth hormone gene polymorphism of local and Holstein cattle breeds in Kerman province of Iran using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Afr. J. Biotechnol. 9(41):6848-6852.

 
 

Mohammadi A, Nassiry MR, Mosafer J, Mohammadabadi MR, Sulimova GE (2009). Distribution of BoLA-DRB3 allelic frequencies and identification of a new allele in the Iranian cattle breed Sistani (Bos indicus). Russ. J. Genet. 45(2):198-202.
Crossref

 
 

Moradband F, Rahimi G, dan Gholizadeh M (2011). Association of Polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep. Asian Aust. J. Anim. Sci. 24(9):1179-1183.
Crossref

 
 

Moradian C, Mohamadi N, Sheshdeh SAR, Hajihosseinlo A and Ashrafi F (2013). Effects of genetic polymorphismat the growth hormone gene on growth traits in Makooei sheep. Eur. J. Exp. Biol. 3(3):101-105.

 
 

Mousavizadeh A, Mohammadabadi MR, Torabi A, Nassiry MR, Ghiasi H, Esmailizadeh AK (2009). Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Iran. J. Biotechnol. 7(1):51-53.

 
 

Nei M (1987). Molecular Evolutionary Genetics. Columbia University Press, New York.

 
 

NseAbasi N, Etim, Mary AO (2014). Environmental and Management Stressors: Implications for Reproductive and Productive Performances of Farm Animals in the Tropics. J. Agric. Sustain. 5(2):153-170.

 
 

Pereira AP, Alencar De, De MM, Oliveira HN, de Regitano LC (2005). Association of GH and IGF-1 polymorphisms with growth traits in a synthetic beef cattle breed. Genet. Mol. Biol. 28(2):230-236.
Crossref

 
 

Popoola MA, Bolarinwa MO, Yahaya MO, Adebisi GL, Saka AA (2014). Thermal comfort effects on physiological adaptations and growth performance of West African dwarf goats raised in Nigeria. Eur. Sci. J. 3:275-281.

 
 

Puja IK, Wandia I N, Suastika P, Sulabda IN (2013). Asosiasi polimorfisme genetika lokus deoxynucleic acid (dna) mikrosatelit gen bovine lymphocyte antigen (BoLA) dengan kualitas semen pada sapi Bali. Jurnal Kedokteran Hewan. 7(2):163-165.
Crossref

 
 

Rahayu S, Sumitro SB, Susilawati T, Soemarno (2006). Identifikasi Polimorfisme Gen GH (Growrth Hormone) Sapi Bali dengan Metode PCR-RFLP. Berk. Penel. Hayati: 12 hal. 7-11.

 
 

Sambrook J, Russell D (2001). Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.

 
 

Sanger F, Nicklen S, Coulson AR (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74(12):5463-5467.
Crossref

 
 

Weir BS (1996). Genetic Data Analysis: Methode for Discrate Population Genetic Data. 2nd ed. Sinauer Associates. Sunderland, MA USA.

 

 


APA Depison, Anwar, S., Jamsari, Arnim, & Yurnalis (2017). Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province. African Journal of Biotechnology , 16(20), 1159-1167.
Chicago Depison, Sarbaini Anwar, Jamsari, Arnim and Yurnalis. "Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province." African Journal of Biotechnology 16, no. 20 (2017): 1159-1167.
MLA Depison, et al. "Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province." African Journal of Biotechnology 16.20 (2017): 1159-1167.
   
DOI 10.5897/AJB2016.15783
URL http://academicjournals.org/journal/AJB/article-abstract/9F56ED264279

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