African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12049

Full Length Research Paper

Fruit, seed and embryo development of different cassava (Manihot esculenta Crantz) genotypes and embryo rescue

Huabing Yan*
  • Huabing Yan*
  • Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, P.R. China; Cash Crop Research Institute, Guangxi Academy of Agricultural Sciences (GXAAS), Nanning, P.R. China; International Center for Tropical Agriculture (CIAT), Km. 17 Recta, Cali-Palmira, Colombia.
  • Google Scholar
Liuying Lu
  • Liuying Lu
  • Cash Crop Research Institute, Guangxi Academy of Agricultural Sciences (GXAAS), Nanning, P.R. China.
  • Google Scholar
Adriana Alzate
  • Adriana Alzate
  • International Center for Tropical Agriculture (CIAT), Km. 17 Recta, Cali-Palmira, Colombia.
  • Google Scholar
Hernan Ceballos
  • Hernan Ceballos
  • International Center for Tropical Agriculture (CIAT), Km. 17 Recta, Cali-Palmira, Colombia.
  • Google Scholar
Clair Hershey
  • Clair Hershey
  • International Center for Tropical Agriculture (CIAT), Km. 17 Recta, Cali-Palmira, Colombia.
  • Google Scholar
Songbi Chen
  • Songbi Chen
  • Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, P.R. China.
  • Google Scholar
Kaimian Li
  • Kaimian Li
  • Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, P.R. China.
  • Google Scholar


  •  Received: 21 August 2013
  •  Accepted: 24 March 2014
  •  Published: 02 April 2014

Abstract

Fruit, seed and embryo developments of different cassava (Manihot esculenta Crantz) genotypes, as well as embryo rescue, were investigated. The fruits of three genotypes after uncontrolled open pollination presented the same progressive development with similar sizes at different stages. There are large differences in the fruit set as well as the embryo development between different genotypes. Days after pollination (DAP) was found not to be an adequate predictor of embryo size as their size ranged from almost invisible to 8.7 mm in length at 32 DAP even within the different locules of the same fruit. The ideal stage for embryo rescue in cassava was from 32 to 36 DAP, because at that stage most embryos are visible (> 0.7 mm); and their excision without injury is feasible. Also, in vitro germination of the cotyledonary embryos at that stage had a high success rate. A half Murashige and Skoog (MS) medium supplemented with 1.0 mg/l GA3, 2% sucrose and 0.2% gel rite proved to be adequate for embryo rescue.

 

Key words: Manihot esculenta Crantz, day after pollination (DAP), fruit set, seed size, embryo size, embryo rescue.