African Journal of Biotechnology
Subscribe to AJB
Full Name*
Email Address*

Article Number - D5555AD62242


Vol.16(1), pp. 32-40 , January 2017
DOI: 10.5897/AJB2016.15676
ISSN: 1684-5315



Full Length Research Paper

Isolation and characterization of heavy metal tolerant bacteria from Panteka stream, Kaduna, Nigeria and their potential for bioremediation



Elizabeth Chika Nwagwu*
  • Elizabeth Chika Nwagwu*
  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
  • Google Scholar
Victoria Moltong Yilwa
  • Victoria Moltong Yilwa
  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
  • Google Scholar
Nkechi Eucharia Egbe
  • Nkechi Eucharia Egbe
  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
  • Google Scholar
Godwin Bryan Onwumere
  • Godwin Bryan Onwumere
  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria.
  • Google Scholar







 Received: 16 September 2016  Accepted: 02 December 2016  Published: 04 January 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Panteka stream is a flowing stream polluted with wastes from the activities of mechanics. Water samples collected at different points of the stream were analysed in order to determine the level of heavy metal contamination and bacteria diversity with the view to elucidating the bioremediating potentials of the bacteria isolates. Four bacteria, tolerant to heavy metals, were isolated from Panteka stream. These were identified by morphological and biochemical techniques as Staphylococcus epidermidis, Serratia marcescens, Proteus mirabilis and Escherichia coli. The 16S rRNA gene sequencing and Basic Local Alignment search tool (BLAST) result confirmed E. coli and Staphylococcus spp. as heavy metal tolerant bacteria. Heavy metal tolerance analysis of the isolates exposed to nickel, zinc, lead, cadmium and iron showed that the isolates had maximum tolerance to the four heavy metal. Studies on bioremediation potential of the isolates to heavy metals in the stream revealed that mixed bacteria culture completely removed lead, nickel, zinc and cadmium. Analysis of pure isolates revealed S. epidermidis to be the most effective in removing lead (100%), nickel (100%), cadmium (90.29%), zinc (84.95%) and iron (54.82%). The results obtained from this study show that all four bacteria species isolated from Panteka stream have potential for bioremediation of heavy metals in contaminated water.

 

Key words: Panteka stream, mechanic workshop, heavy metals, bacteria isolates, bioremediation.

Alam M, Nadeem R, Jilani M I (2012). Pb (II) removal from wastewater using Pomegranate waste biomass. Int. J. Chem. Biochem. Sci.1:48-53.

 

Fosmire G J (1990). Zinc toxicity. Am. J. Clin. Nutr. 51(2):225-227.

 

Grass G, Fan B, Rosen BP, Franke S, Nies DH, Rensing C (2001). ZitB (YbgR), a member of the cation diffusion facilitator family, is an additional zinc transporter in Escherichia coli. J. Bacteriol. 183:4664-4667.
Crossref

 

Gupta AK, Yunus M, Pandey P (2003). Bioremediation in ecotechnology for the present century. International Society of Environmental Botanists 9:2.

 

Hamlett NV, Landale EC, Davis BH, Summers AO (1992). Roles of the Tn21 merT, merP, and merC gene products in mercury resistance and mercury binding. J. Bacteriol. 174:6377-6385.
Crossref

 

Jyothi K, Surendra BK, Nancy CK, Kashyap A (2012). Identification and Isolation of Hydrocarbon Degrading Bacteria by Molecular Characterization. Helix 2:105-111.

 

Kenneth HW, Rhonda BB, Ronald CG (1990). Amplification of Bacterial 16S Ribosomal DNA with Polymerase Chain Reaction. J. Clin. Microbiol. 28:1942-1946.

 

Kolawole SE, Obueh HO (2015). Evaluation of the minerals, heavy metals and microbial compositions of drinking water from different sources in Utagba-Uno, Nigeria. J. Health Environ. Sci. 2:6-10.
Crossref

 

Lawford RG, Landwehr JM, Sorooshian S, Whitaker MPL (2003). International Hydrologic Science Programs and Global Water Issues. Water: Science, Policy and Management. Water Resources Monograph 16. American Geophysical Union 10.

 

Li J, Xie ZM, Xu JM, Sun, YF (2006). Risk assessment for safety of soils and vegetables around a lead/zinc mine. Environ. Geochem. Health 28:37-44.
Crossref

 

Manton WI, Angle CR, Stanek KL, Reese YR, Kuehnemann TJ (2000). Acquisition and retention of lead by young children. Environ. Res. 82:60-80.
Crossref

 

Martin-Gonzalez A, Díaz S, Borniquel S, Gallego A, Gutierrez JC (2006). Cytotoxicity and bioaccumulation of heavy metals by ciliated protozoa isolated from urban wastewater treatment plants. Res. Microbiol. 157:108-118.
Crossref

 

McConnell JR, Edwards R (2008). Coal burning leaves toxic heavy metal legacy in the Arctic. Proc. Natl. Acad. Sci. U. S. A. 105:12140-12144.
Crossref

 

Nageswaran N, Ramteke, PW, Verma OP, Pandey A (2012). Antibiotic Susceptibility and Heavy Metal Tolerance Pattern of Serratia marcescens Isolated From Soil and Water. J. Bioremediat. Biodegrad. 3:158.
Crossref

 

Nwidu LL, Oveh B, Okoriye T, Vaikosen NA (2008). Assessment of the water quality and prevalence of water borne diseases in Amassoma, Niger Delta, Nigeria. Afr. J. Biotechnol. 7(17):2993-2997.

 

Oaikhena EE, Makaije DB, Denwe SD, Namadi MM, Haroun AA (2016). Bioremediation potentials of heavy metal tolerant bacteria isolated from petroleum refinery effluent. Am. J. Environ. Protect. 5(2):29-34.
Crossref

 

Owolabi JB, Hekeu MM (2015). Isolation and characterization of zinc resistance bacteria from a coil coating industrial wastewater treatment plant. Int. J. Environ. Sci. 5(5):1030-1042.

 

Pandit R, Patel B, Kunjadia P, Nagee A (2013). Isolation, characterization and molecular identification of heavy metal resistant bacteria from industrial effluents. Amala-khadi-Ankleshwar, Gujarat. Int. J. Environ. Sci. 3(5):1689-1699.

 

Simon CA (1998). Iron storage in bacteria. Adv. Microb. Physiol. 40:281-35.
Crossref

 

Umrania VV (2006). Bioremediation of toxic heavy metals using acidothermophilic autotrophes. Bioresour. Technol. 97:1237-1242.
Crossref

 

Valls M, Lorenzo VD (2002). Exploiting the genetic and biochemical capacities of bacteria for the remediation of heavy metal pollution. FEMS Microbiol. Rev. 26:327-338.
Crossref

 

White C, Gadd, GM. (1998). Accumulation and effects of cadmium on sulphate-reducing bacterial biofilms. Microbiol. 144:1407-1415.
Crossref

 

WHO-World Health Organization: The World health report (2007). A safer future: global public health security in the 21st century.

 

Yaoa J, Tiana L, Wanga Y, Djaha A, Wanga F, Chena H, Sua C, Zhuanga R, Zhoua Y, Choib MMF, Bramantic E (2008). Microcalorimetric study the toxic effect of hexavalent chromium on microbial activity of Wuhan brown sandy soil: an in vitro approach. Ecotoxicol. Environ. Safe. 69:89-95.
Crossref

 


APA Nwagwu, E. C., Yilwa, V. M., Egbe, N. E., & Onwumere, G. B. (2017). Isolation and characterization of heavy metal tolerant bacteria from Panteka stream, Kaduna, Nigeria and their potential for bioremediation. African Journal of Biotechnology , 16(1), 32-40.
Chicago Elizabeth Chika Nwagwu, Victoria Moltong Yilwa, Nkechi Eucharia Egbe and Godwin Bryan Onwumere. "Isolation and characterization of heavy metal tolerant bacteria from Panteka stream, Kaduna, Nigeria and their potential for bioremediation." African Journal of Biotechnology 16, no. 1 (2017): 32-40.
MLA Elizabeth Chika Nwagwu, et al. "Isolation and characterization of heavy metal tolerant bacteria from Panteka stream, Kaduna, Nigeria and their potential for bioremediation." African Journal of Biotechnology 16.1 (2017): 32-40.
   
DOI 10.5897/AJB2016.15676
URL http://academicjournals.org/journal/AJB/article-abstract/D5555AD62242

Subscription Form