African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12024

Full Length Research Paper

Electrolyte ions and glutathione enzymes as stress markers in Argania spinosa subjected to drought stress and recovery

Abdelghani Chakhchar*
  • Abdelghani Chakhchar*
  • Laboratoire de Biotechnologie Valorisation et Protection des Agroressources, Faculté des Sciences et Techniques Guéliz, Université Cadi Ayyad, 40 000 Marrakech, Maroc.
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Mouna Lamaoui
  • Mouna Lamaoui
  • Laboratoire de Biotechnologie Valorisation et Protection des Agroressources, Faculté des Sciences et Techniques Guéliz, Université Cadi Ayyad, 40 000 Marrakech, Maroc.
  • Google Scholar
Salama Aissam
  • Salama Aissam
  • Laboratoire de Biotechnologie Valorisation et Protection des Agroressources, Faculté des Sciences et Techniques Guéliz, Université Cadi Ayyad, 40 000 Marrakech, Maroc.
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Abderrahim Ferradous
  • Abderrahim Ferradous
  • Centre Régional de la Recherche Forestière Marrakech, BP 12360 Ain Itti Ennakhil, Marrakech, Maroc.
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Said Wahbi
  • Said Wahbi
  • Laboratoire de Biotechnologie et Physiologie Végétale, Faculté des Sciences Semlalia, Université Cadi Ayyad, 40 000 Marrakech, Maroc.
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Abdelhamid EI Mousadik
  • Abdelhamid EI Mousadik
  • Laboratoire de Biotechnologie et Valorisation des Ressources Naturelles, Faculté des Sciences, Université Ibn Zohr, Agadir, Maroc.
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Saad Ibnsouda-Koraichi
  • Saad Ibnsouda-Koraichi
  • Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques, Université Sidi Mohamed Ben Abdellah, Fès, Maroc.
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Abdelkarim Filali-Maltouf
  • Abdelkarim Filali-Maltouf
  • Laboratoire de Microbiologie et Biologie Moléculaire, Faculté des Sciences, Université Mohammed V Agdal, Rabat, Maroc.
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Cherkaoui El Modafar
  • Cherkaoui El Modafar
  • Laboratoire de Biotechnologie Valorisation et Protection des Agroressources, Faculté des Sciences et Techniques Guéliz, Université Cadi Ayyad, 40 000 Marrakech, Maroc.
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  •  Received: 22 January 2016
  •  Accepted: 13 May 2016
  •  Published: 04 January 2017

Abstract

Understanding the mechanisms underlying Argania spinosa responses to drought stress is essential for its regeneration and domestication. Toward that end, an integrative study of tolerance responses to drought stress in four A. spinosa ecotypes (2 contrasting coastal ecotypes (Adm and Rab) and 2 contrasting inland ecotypes (Alz and Lks)) have been conducted. Responses to soil drying and re-watering were measured at physiological and biochemical levels. Soil drying resulted in significant increase in leaf concentrations of potassium (K+), calcium (Ca2+) and magnesium (Mg2+) with differential responses between ecotypes. The glutathione-related enzymes: glutathione peroxidase (GP), glutathione reductase (GR) and glutathione S-transferase (GST) showed a significant increase in their enzymatic activity in A. spinosa plants subjected to drought stress. Additionally, a significant increase in thiol protein content in the four ecotypes was recorded, during drought stress. These antioxidant traits responded differently depending on ecotype. However, rapid and significant changes in the studied physiological and biochemical traits were observed during recovery from drought, only after four days. According to the traits having the most discriminating power, the both inland ecotypes, especially Lks ecotype, seem to be potential candidates for regeneration of argan forest and their domestication in arid and semi-arid environments.

Key words: Argania spinosa, drought stress, glutathione enzymes, thiol compounds, recovery.