February 2018
Isolation, purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus thuringinsis-SH-II-1A
In the present study, the isolation, purification and partial characterization of thermostable serine alkaline protease produced from Bacillus thuringinsis SH-II-1A was reported. The culture was isolated from soil of slaughter house waste and identified further from ribosomal sequence. The crude enzyme was purified by ammonium sulfate precipitation, dialysis and Sephadex G-200 gel permeation chromatography up to 17.04...
February 2018
Cucurbit yellow stunting disorder virus and watermelon chlorotic stunt virus induced gene silencing in tobacco plants
Cucurbit yellow stunting disorder virus (CYSDV) and Watermelon chlorotic stunt virus (WmCSV) are the most widespread and damaging viruses to cucurbits in the Middle East. CYSDV and WmCSV are cucurbit-infecting bipartite whitefly-transmitted viruses. Post-transcriptional gene silencing (PTGS) is a universal mechanism by which plants are able to systemically switch off the expression of targeted genes via the reduction...
February 2018
Tomato yellow leaf curl virus: Diagnosis and metabolites
The existence of Tomato yellow leaf curl virus (TYLCV) was figured out in different locations in Al-Ahsaa of Saudi Arabia. Polymerase chain reaction (PCR) results of samples collected showed that TYLCV existed in all locations. Using AVcore and ACcore primers, begomoviruses family were detected in symptomatic tomato plants and by using TYv2664 and TYc138 (specific primers for the detection of TYLCV), the results proved...
February 2018
Polymerase chain reaction amplification of 16S rDNA from three nosocomial bacterial isolates in Kaduna State, Northern Nigeria
A wide variety of opportunistic pathogens has been detected in hospital surfaces. Medical center surfaces can serve as reservoirs of pathogenic bacteria. Among this pathogens, Pseudomonas species are one of the leading causes of nosocomial infections, frequently found in hospital environments. Polymerase chain amplification system remains one of the best methods for the rapid detection of low numbers of pathogenic...
February 2018
The nitrogen-fixing Frankia significantly increases growth, uprooting resistance and root tensile strength of Alnus formosana
Restoration of Alnus formosana (Burk.) Makino on landslide areas is important for agroforestry, forestry and soil erosion control in Taiwan. To ensure successful reforestation, A. formosana seedlings have to develop strong root system for nutrient and water acquisition as well as anchorage. Inoculating of A. formosana with symbiotic nitrogen-fixing actinobacteria Frankia may help mitigate drought and nutrient...
February 2018
Characterization of biodiesel obtained from atemoya (Annona squamosa × A. cherimola) seed oil
Biodiesel is derived from renewable sources, such as vegetable oils, by means of a transesterification process in which triacylglycerols are transformed into smaller molecules of esters of fatty acids and glycerol. The transesterification reactions of ‘Gefner’ atemoya (Annona squamosa × A. cherimola) seed oil extracted by pressing (physical) and solvent (chemical) processes were studied, with analysis...
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