African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12479

Table of Content: 15 April 2015; 14(15)

April 2015

Allelic frequencies for the seventeen Y-STR loci observed in Iraqi male patients with prostate cancer

Prostate cancer is a significant disease in men and a large number of individuals would benefit if risk factors that increase the susceptibility to develop this neoplasia could be established, which could aid in the early detection of the disease that is crucial for successful treatment. The first objective of this study was detection of allelic frequencies of 17 Y-chromosome short tandem repeat loci from Iraqian...

Author(s): Imad Hadi Hameed, Mohammed Abdullah Jebor and Muhanned Abdulhasan Kareem

April 2015

Morphological and RAPD-marker characterization of Melia volkensii (Gürke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Somaclonal variation induced in vitro during tissue culture can be a problem in clonal micropropagation of elite plants. This study investigated the extent of morphological and genetic similarity or dissimilarity between Melia volkensii in vitro plants (somatic seedlings) obtained via somatic embryogenesis and normal seedlings. Comparisons were made between in vitro plants regenerated directly from cotyledon explants,...

Author(s): Eliud Sagwa Mulanda, Yeremia Chuhila, Ryan Musumba Awori, Mark Ochieng Adero, Nelson Onzere Amugune, Elijah Akunda and Jenesio Ikindu Kinyamario

April 2015

Effect of temperature, salinity, light and time of dehiscence on seed germination and seedling morphology of Calotropis procera from urban habitats

Calotropis procera (giant milkweed) is a hardy xerophytic plant, which is distributed globally in many countries and has important economic and ecological functions. The present study aimed at estimating the effect of temperature, salinity and time of fruit dehiscence on the seed germination and seedling morphology of giant milkweed in urban habitats. Seeds were collected early (in June) and late (in December) from...

Author(s): Tarek M. Galal, Emad A. Farahat, Maha M. El-Midany and Loutfy M. Hassan

April 2015

Influence of various carbohydrates on the in vitro micropropagation of Nauclea diderrichii (De Wild &T. Durand) Merrill, an endangered forest species in Togo

The over exploitation of Nauclea diderrichii, for its very resistant wood against the attacks of fungus (Coriolus versicolor), Lyctus, termites (Reticulitermes santonensis) and marine borers, leads to its disappearance in Togo. This forest species produces many fruits containing numerous seeds; unfortunately their seedling is laborious in its biotope. In vitro micropropagation trials were carried out for a faster and...

Author(s): PITEKELABOU Rassimwaï, , AÏDAM Atsou Vincent and KOKOU Kouami

April 2015

Establishment of an efficient callus induction method from leaf and stem in kinnow mandarin (Citrus reticulata Blanco.) and citron (Citrus medica L.)

Kinnow mandarin (Citrus reticulata Blanco.) is a highly adaptable variety among citrus cultivars in the climate of the region of Panjab, Pakistan and citron (Citrus medica L.) was the first of the citrus fruits known to become visible in the Mediterranean Basin. In Sylhet region of Bangladesh, it is commonly known as Zara lemon. The establishment of in vitro technique for seed germination and callus induction has been...

Author(s): Mohammad Kamruzzaman, Arfa Akther, Md. Omar Faruq, Afroza Pervin, Sanat Myti and Shamsul H. Prodhan

April 2015

Synthesis and characterization of 2-mercapto-N-methyl imidazole substituted benzimidazole derivatives and investigation of their effect on production of plantlets in Oncidium Gower Ramsey

A series of benzimidazole derivatives (5a-c) were synthesized by coupling 5-substituted 2-chloromethyl benzimidazole (3a-c) with 2-mercapto-N-methyl imidazole. The synthesized compounds were characterized by infra-red (IR), nuclear magnetic resonance (NMR) and elemental analyses. Further, the synthesized compounds were tested on plantlet production from protocorm like bodies (PLBs) sections of Oncidium. PLBs sections...

Author(s): Mahesh R,#, Ramya K,#, Ashok Kumar HG,# and Satyanarayana S,

April 2015

Production of lignocellulolytic enzymes from three white-rot fungi by solid-state fermentation and mathematical modeling

Three species of white rot-fungi (Pleurotus ostreatus, Coriolus versicolor, and Lentinula edodes) were grown on 12 solid media based on several lignocellulosic materials (oak sawdust, coconut husks, coffee husks and corn bran) during 49 days. The media had varied carbon/nitrogen ratios and CuSO4 content. The objective of the work was to evaluate the effect of the media formulation on the production of lignocellulolytic...

Author(s): Sandra Montoya, Óscar J. Sánchez and Laura Levin

April 2015

Assessment of acute toxicity profile of Lasianthera africana leaf extract in normal rats and its ameliorative effect in alloxan-induced diabetic rats

The present study evaluated the acute toxicity effect of Lasianthera africana leaf extract on normal rats and effect of the extract on the hepatic and renal function indices in alloxan-induced diabetic rats. In the acute toxicity test, male Wister rats received orally four different single dose of the extract and were kept under observation for 14 days. The result indicates that LD50 of the extract is higher than 5,000...

Author(s): U. E. Inyang, P. A. Nwafor and U. N. Asamudo

April 2015

Electrophoretic analysis of proteins from different date palm (Phoenix dactylifera L.) cultivars in Saudi Arabia

Fifteen (15) samples of different date palm cultivars were collected from different locations in Al-Ahsa oasis in the eastern province of Saudi Arabia. Extracted proteins from these samples were analyzed by electrophoresis, and clustered according to the average linkage between groups hierarchical clustering method. The results reveal high degree of similarity based on Jaccard`s similarity method on the basis of...

Author(s): Al-Issa Adil Mohammad

April 2015

Influence of breeds genetic composition on the quality of milk from primiparous cows

Forty-four crossbred primiparous cows participated in this study, namely: 15 1/2 animals corresponding to 1/2 Holstein (HO) x 1/2 Gyr; 15 3/4 animals corresponding to (3/4 HO x 1/4 Gyr) and 14 7/8 animals corresponding to (7/8 HO x 1/8 Gyr) raised under similar handling and feeding conditions. The sample collection was carried out between January and March 2013. To compare the quality of milk among breeds genetic...

Author(s): Melo, A. F., Silva, M. A. P., Silva, F. R., Carmo, R. M., Neves, R. B. S., Nicolau, E. S. and Lage, M. E.