February 2018

Extraction of high quality RNA from hard tissues of adult coconut palms

An experimental protocol originally designed to isolate plant DNA was modified to obtain high quality total RNA from organs of adult coconut palms collected in situ. With this protocol, high quality RNA was extracted from leaves, inflorescences, primary and secondary roots, zygotic embryos and solid endosperm, with no carbohydrate or protein contamination. Reverse transcription-polymerase chain reaction (RT-PCR)...

Author(s): Ramón Souza-Perera, Nelson Torres-Hernández and José Juan Zúñiga-Aguilar

February 2018

Shell morphology and the radula structures of two closely related bulinid snails intermediate host of Schistosoma haematobium in Nigeria

Bulinid snails act as intermediate host of schistosomes and the presence of the snail gives schistosomiasis an expansive characteristics. Schistosomiasis is of medical and veterinary importance in the tropical and subtropical regions. The shell shape and structure of radula teeth of bulinids are often specific to a species or genus, and are widely used for gastropod species identification. Bulinid species collected from...

Author(s): Opeyemi Gbenga Oso and Alexander Bababunmi Odaibo

February 2018

Effect of water deficit at different stages of development on the yield components of cowpea (Vigna unguiculata L. Walp) genotypes

Cowpea cultivation is widespread in West Africa where it is an important source of protein. This study is aimed at determining the effects of water deficit applied at different stages of cowpea development on yield and its components (pod number, seed number, seed yield, aerial biomass yield, harvest index and root biomass of the plant). The experiments were carried out in pots during the rainy season of 2016 under...

Author(s): TOUDOU DAOUDA Abdoul Karim, ATTA Sanoussi, INOUSSA Maman Maârouhi, HAMIDOU Falalou and BAKASSO Yacoubou  

February 2018

Maximizing L-glutaminase production from marine Bacillus subtilis JK-79 under solid state fermentation

L-Glutaminase is majorly produced by microorganism including bacteria, yeast and fungi. It mainly catalyzes the hydrolysis of gamma-amido bond of L-glutamine. In the present investigation, the potent marine isolate, Bacillus subtilis JK-79 producing L-glutaminase was evaluated for the maximum L-glutaminase production by solid state fermentation (SSF). In this context, different agro-industrial residues reported in...

Author(s): Jambulingam Kiruthika, Nachimuthu Saraswathy and Saranya Murugesan