African Journal of Biochemistry Research
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Article Number - 23BD8B461518


Vol.10(7), pp. 47-58 , November 2016
DOI: 10.5897/AJBR2015.0862
ISSN: 1996-0778



Full Length Research Paper

Extraction, optimization and characterization of crude polysaccharides from Artemesia Mongolica



Yahaya Kudush Kawa*
  • Yahaya Kudush Kawa*
  • Department of Chemistry Njala University, Sierra Leone.
  • Google Scholar
Quinck Shang
  • Quinck Shang
  • College of Chemistry, Northeast Normal University, Changchun, China.
  • Google Scholar
Shangyi Tian
  • Shangyi Tian
  • College of Chemistry, Northeast Normal University, Changchun, China.
  • Google Scholar
John Paul Kaisam
  • John Paul Kaisam
  • Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, China.
  • Google Scholar
Victoria Kabia
  • Victoria Kabia
  • Department of Chemistry Njala University, Sierra Leone.
  • Google Scholar







 Received: 30 August 2015  Accepted: 08 January 2016  Published: 30 November 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In this study, the extraction conditions of polysaccharides of Artemisia mongolia (PAM) were optimized and their molecular weight, weight distribution, composition and antioxidant activities of the polysaccharides investigated. The degree of polymerization of fructan-oligosaccharides 2-8 was separated by thin layer chromatography (TLC) with good results. Polysaccharides were extracted from the leaves of A. mongolia and the effects of the extraction parameters (extraction temperature, extraction time and water to raw material ratio) were optimized under optimal extraction combinations. Based on the study, the maximum yield of polysaccharides (17.38%) was obtained at an extraction temperature of 76.90°C, extraction time of 1.33 h and water-to-raw material ratio of 4.00 ml/mg. The yield of polysaccharides was largely dependent on the extraction parameters. Four isolated fractions of the polysaccharides were further characterized using high-performance liquid chromatography. Using TLC analysis, the degree of polymerization of fructan-oligosaccharides 2-8 was successfully separated. High A. mongolia antioxidant polysaccharide with antioxidant activity of 351.3 sc/g was obtained at optimized incubation temperature of 70.37°C, extraction time of 1.45 h and water-to-raw material ratio of 2 ml/mg.     

Key words: Artemisia Mongolia, crude polysaccharide, antioxidant activity, response surface methodology, box-Behnken design  

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APA Kawa, Y. K., Shang, Q., Tian, S., Kaisam, J. P., & Kabia, V. (2016). Extraction, optimization and characterization of crude polysaccharides from Artemesia Mongolica. African Journal of Biochemistry Research , 10(7), 47-58.
Chicago Yahaya Kudush Kawa, Quinck Shang, Shangyi Tian, John Paul Kaisam and Victoria Kabia. "Extraction, optimization and characterization of crude polysaccharides from Artemesia Mongolica." African Journal of Biochemistry Research 10, no. 7 (2016): 47-58.
MLA Yahaya Kudush Kawa, et al. "Extraction, optimization and characterization of crude polysaccharides from Artemesia Mongolica." African Journal of Biochemistry Research 10.7 (2016): 47-58.
   
DOI 10.5897/AJBR2015.0862
URL http://academicjournals.org/journal/AJBR/article-abstract/23BD8B461518

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