African Journal of Biochemistry Research
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Article Number - 57ADE679193


Vol.2(1), pp. 008-014 , January 2008

ISSN: 1996-0778



Full Length Research Paper

Gangliosides as potential inhibitors of Naja naja venom PLA2 (NV-PLA2) induced human erythrocyte membrane damage


Mukunda Chethankumar and Leela Srinivas*




Adichunchanagiri Biotechnology and Cancer Research Institute, Balagangadharanatha Nagara – 571 448, Karnataka, India.


Email: leela_srivas@yahoo.co.uk






 Accepted: 29 January 2007  Published: 31 January 2008

Copyright © 2008 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


We determined the ability of mixed gangliosides (16% GD1b, 19% GT1b, 21% GM1, and 40% GD1a) and individual gangliosides GM1 and GD1b to modulate the NV-PLAinduced human erythrocyte ghost membrane damage. CM-Sephadex purification of crude Naja naja venom yielded eight peaks of which peak VII, a major phospholipase A2 (NV-PLA2) accounted for 22% of the total protein recovered and 8% of the total PLAactivity recovered. The membrane damage induced by NV-PLAwas assessed by measuring the decrease in the relative intensity of fluorescence using cis–parinaric acid (PnA) as a monitor molecule. The RBC membranes isolated from healthy human blood showed 72% damage on treatment with NV-PLA(2 mg) when compared to untreated membranes. Mixed gangliosides (18 nM) and GM1 (15 nM) offered 81 and 86% protection respectively, whereas GD1b (20 nM) did not show significant protection. Analysis of membrane bound Na+K+ and Ca2+Mg2+ ATPase indicated a 3 fold and 2 folds decrease in their activities on NV-PLAtreatment when compared to untreated membranes. Mixed gangliosides restored the Na+K+ ATPase activity by 78%, whereas GM1 and GD1b offered 74 and 52% restoration respectively. The Ca2+Mg2+ ATPase activity was restored by 80 and 81% with mixed gangliosides and GM1 respectively. GD1b showed only 50% restoration. Mixed gangliosides and GM1 exhibited a significant dose dependent inhibition of NV-PLAactivity when compared to GD1b. The data show that mixed gangliosides and GM1 were effective in modulating NV-PLA2induced erythrocyte membrane damage than GD1b.

 

Key words: Gangliosides, NV-PLA2, PnA, gangliosides, erythrocyte membrane, NaK+ ATPase, Ca2+ Mg2+ ATPase.


APA (2008). Gangliosides as potential inhibitors of Naja naja venom PLA2 (NV-PLA2) induced human erythrocyte membrane damage. African Journal of Biochemistry Research , 2(1), 008-014.
Chicago Mukunda Chethankumar and Leela Srinivas. "Gangliosides as potential inhibitors of Naja naja venom PLA2 (NV-PLA2) induced human erythrocyte membrane damage." African Journal of Biochemistry Research 2, no. 1 (2008): 008-014.
MLA Mukunda Chethankumar and Leela Srinivas. "Gangliosides as potential inhibitors of Naja naja venom PLA2 (NV-PLA2) induced human erythrocyte membrane damage." African Journal of Biochemistry Research 2.1 (2008): 008-014.
   
DOI
URL http://academicjournals.org/journal/AJBR/article-abstract/57ADE679193

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