African Journal of Environmental Science and Technology
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Article Number - 80805E911143


Vol.1(1), pp. 001-006 , August 2007

ISSN: 1996-0786



Full Length Research Paper

Discrimination of Scutellospora castanea from arbuscular mycorrhizal fungi isolated in TENE primary forest (Centre west Côte d’Ivoire) and detection of repeated DNA sequences from its genome


Adolphe Zézé1*, Yao Casimir Brou1, Diederick Van Tuinen2




 

1Laboratoire d’Agronomie, Ecole Supérieure d’Agronomie, Institut National Polytechnique Houphouet-Boigny, BP 1313  Yamoussoukro, Côte d’Ivoire.

2Unité Mixte de Recherche INRA/Université de Bourgogne, Biochimie, Biologie Cellulaire et Ecologie des Interactions Plantes/Microorganismes, CMSE-INRA, BP 86510, 21065 Dijon Cédex, France.


Email: zomure@yahoo.com






 Accepted: 12 July 2007  Published: 30 August 2007

Copyright © 2007 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

Primers SC1 and SC2 primarily developed to be specific to Scutellospora castanea were used to unambiguously discriminate this species among endogenous arbuscular mycorrhizal fungi collected in Côte d’Ivoire. The cloning of genomic DNA from this fungus allowed the isolation of repeated DNA sequences. Sixteen inserts were randomly selected from the genomic bank and hybridized with total DNA from the spores of the identified fungus as probe. Repeated DNA sequences were obtained in the form of intense signals. Five fragments gave intense signals of hybridization showing that they are repeated elements in the genome. One of these sequences ScaCI2 having 1000 pb was characterized. It is dispersed in the genome of this AM fungus and accounts for 0.03% of this one.

 

Key words: Arbuscular mycorrhizal fungi, Scutellospora castanea, Côte d’Ivoire, detection.

 

 

 


APA (2007). Discrimination of Scutellospora castanea from arbuscular mycorrhizal fungi isolated in TENE primary forest (Centre west Côte d’Ivoire) and detection of repeated DNA sequences from its genome. African Journal of Environmental Science and Technology , 1(1), 001-006.
Chicago Adolphe Z&ez&e, Yao Casimir Brou, Diederick Van Tuinen. "Discrimination of Scutellospora castanea from arbuscular mycorrhizal fungi isolated in TENE primary forest (Centre west Côte d’Ivoire) and detection of repeated DNA sequences from its genome." African Journal of Environmental Science and Technology 1, no. 1 (2007): 001-006.
MLA Adolphe Z&ez&e, Yao Casimir Brou, Diederick Van Tuinen. "Discrimination of Scutellospora castanea from arbuscular mycorrhizal fungi isolated in TENE primary forest (Centre west Côte d’Ivoire) and detection of repeated DNA sequences from its genome." African Journal of Environmental Science and Technology 1.1 (2007): 001-006.
   
DOI
URL http://academicjournals.org/journal/AJEST/article-abstract/80805E911143

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