African Journal of
Environmental Science and Technology

  • Abbreviation: Afr. J. Environ. Sci. Technol.
  • Language: English
  • ISSN: 1996-0786
  • DOI: 10.5897/AJEST
  • Start Year: 2007
  • Published Articles: 878

Full Length Research Paper

Discrimination of Scutellospora castanea from arbuscular mycorrhizal fungi isolated in TENE primary forest (Centre west Côte d’Ivoire) and detection of repeated DNA sequences from its genome

Adolphe Zézé1*, Yao Casimir Brou1, Diederick Van Tuinen2
  1Laboratoire d’Agronomie, Ecole Supérieure d’Agronomie, Institut National Polytechnique Houphouet-Boigny, BP 1313  Yamoussoukro, Côte d’Ivoire. 2Unité Mixte de Recherche INRA/Université de Bourgogne, Biochimie, Biologie Cellulaire et Ecologie des Interactions Plantes/Microorganismes, CMSE-INRA, BP 86510, 21065 Dijon Cédex, France.
Email: [email protected]

  • Article Number - 80805E911143
  • Vol.1(1), pp. 001-006, August 2007
  •  Accepted: 12 July 2007
  •  Published: 30 August 2007

Abstract

 

Primers SC1 and SC2 primarily developed to be specific to Scutellospora castanea were used to unambiguously discriminate this species among endogenous arbuscular mycorrhizal fungi collected in Côte d’Ivoire. The cloning of genomic DNA from this fungus allowed the isolation of repeated DNA sequences. Sixteen inserts were randomly selected from the genomic bank and hybridized with total DNA from the spores of the identified fungus as probe. Repeated DNA sequences were obtained in the form of intense signals. Five fragments gave intense signals of hybridization showing that they are repeated elements in the genome. One of these sequences ScaCI2 having 1000 pb was characterized. It is dispersed in the genome of this AM fungus and accounts for 0.03% of this one.

 

Key words: Arbuscular mycorrhizal fungi, Scutellospora castanea, Côte d’Ivoire, detection.

 

 

 

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