African Journal of
Food Science

  • Abbreviation: Afr. J. Food Sci.
  • Language: English
  • ISSN: 1996-0794
  • DOI: 10.5897/AJFS
  • Start Year: 2007
  • Published Articles: 770

Full Length Research Paper

In vitro characterization of a vancomycin-resistant strain of Leuconostoc lactis isolated from chicken carcasses and its activity against some foodborne pathogens

Hany M. Yehia
  • Hany M. Yehia
  • Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Saudi Arabia.
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Samah Ghanem
  • Samah Ghanem
  • Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia.
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Tahra Elobeid
  • Tahra Elobeid
  • Human Nutrition Department, College of Health Sciences, Qatar University, P. O. Box 2713, Doha, Qatar.
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Sameh Hassan Mosilhey
  • Sameh Hassan Mosilhey
  • Department of Food and Dairy Science and Technology, Faculty of Environmental Agricultural Sciences, Suez Canal University, AlArish, Egypt.
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Ioannis N. Savvaidis
  • Ioannis N. Savvaidis
  • Laboratory of Food Chemistry and Food Microbiology, Department of Chemistry, University of Ioannina, GR-45110, Ioannina, Greece.
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  •  Received: 12 June 2017
  •  Accepted: 17 August 2017
  •  Published: 31 October 2017

Abstract

Recently, increasing attention has been paid on Leuconostoc lactis as a promising bioactive organism against food-borne and spoilage bacteria. A total of nine strains, including six different species of the genus Lactobacillus and three species of the genus Leuconostoc, were isolated from chicken carcasses (n=60) collected from wholesale poultry markets located at Al-Riyadh city, Saudi Arabia in 2016 and identified by API 50 CHL assays. L. lactis isolates were resistant to bile salts and vancomycin. The autolytic phenotype of L. lactis was evaluated under starvation conditions in the presence of potassium phosphate buffer. The strains tested showed partial autolysis of approximately 18% after 7 h of starvation at 37°C at the end of the exponential phase. The inhibitory activity of whole-protein extracts of L. lactis against the foodborne bacteria, Listeria monocytogenes, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus and Micrococcus luteus was evaluated by renaturing sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The major activity of the total protein appeared as two clear bands on the SDS-PAGE, of approximately 57 and 42 kDa against L. monocytogenes, B. cereus and B. subtilis. No active band was shown against S. aureus and M. luteus.

Key words: Bacteriocins, biopreservation, lactic acid bacteria, pathogens, poultry.

Abbreviation

Analytical profile index; SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis; kDa, kilo Dalton; OD, optical density; IU, international Unit; EDTA, ethylenediaminetetraacetic

acid; TES buffer, Tris EDTA buffer; UV, ultra violet.