African Journal of Food Science
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Article Number - 54811B466759


Vol.11(12), pp. 389-396 , December 2017
DOI: 10.5897/AJFS2017.1628
ISSN: 1996-0794



Full Length Research Paper

Assessment of loss of carbohydrate through fermentation process of yeast (Saccharomyces cerevisiae) from small sample of maize flour dough



Kasahun Gudeta
  • Kasahun Gudeta
  • Department of Biology, Wato Preparatory School, P. O. Box 42718, Sebeta, Ethiopia.
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Messele Admassu
  • Messele Admassu
  • Department of Biology, Adama Science and Technology University, P.O. Box 1888, Adama, Ethiopia.
  • Google Scholar







 Received: 13 June 2017  Accepted: 26 July 2017  Published: 31 December 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The time used for fermentation should be limited by bakers, because if fermentation takes long time, the major nutrients in food, especially cereals can be reduced. The aim of this study is to assess the amount of carbohydrate nutrients transformed by yeast cells (Sacharomyces cerevisiae) through the fermentation of maize flour dough. Lane-Eynon and iodine-thiosulfate titration methods were used to quantify the concentration of both simple sugar and starch in the samples. Twelve samples were used for the analysis; 3 were used before the fermentation and another 3 were used after the fermentation at 17, 20 and 23 h. The amounts of starch consumed by yeast cells from the samples after fermentation time (17, 20 and 23 h) were 23.97, 49.13 and 68.45%, respectively. No simple sugar was detected after 17 h of fermentation of the samples. The results revealed that a significant amount of starch was transformed. Therefore, fermentation time should not be extended to prevent loss.

Key words: Maize flour, fermentation, reducing sugar, Saccharomyces cerevisiae, starch, titration.

Akbar A, Aamir S, Moazzam RK, Muhammad A, Muhammad RA (2012). Yeast, its types and role in fermentation during bread making process. Pak. J. Food Sci. 22(3):171-179

 

Alton B, Kathleen G, Whitney CH, Chris K, Linda L, Peter R (2002). The Dynamics of Life. National Geographic Society. Columbus USA. pp. 96-99p.

 
 

Cavalieri D, McGovern PE, Hartl Dl, Mortimer R, Polsinelli M (2003). Evidence for S. cerevisiae fermentation in ancient wine. J. Mol. Evol. 57(1):S226-S232.
Crossref

 
 

Dickinson R (1999). Carbon Metabolism the Metabolism and Molecular Physiology of Saccharomyces cerevisiae. Philadelphia, P. A. Taylor and Francis.

 
 

Dunsmore A, Mellet P, Wolf M (1980). Some Factor Affecting the Lane and Eynon Titration method for determining Reducing Sugars in Sugar products. Sugar Milingling Research Institute. pp. 72-76.

 
 

Gerald K (2003). Cell and Molecular Biology Concepts and Experiment.3rd edition. Johnwiley and sons, inc.New York. 112p.

 
 

International Starch Institute (1999). Determination of reducing power and DE by Lane Eynon's Method. Science Park Aarhus, Denmark.

 
 

James M, Martin J, David A (2005). Food Microbiology. 7th edition.Singer science business media, Inc, 233 spring street, New York. P 31.

 
 

Knutson CA (1999). Evaluation of variation in Amylose. Iodine Absorbance Spectra. Bio. Pros.Res. 42:65-72.

 
 

Madigan MT, Martinko JM, Parker J (2003). Brock Bilogy of Microorganisms, 10th edition, pearson Education Inc.

 
 

Marcin B (2005-2008). Bpp Marcin Borkowiski ul. Archite ktow 14 05-270 Marki poland (http://f 30. X8top. Net retrined on July 15, 2017 Adama.

 
 

McGoven E, Zhang J, Zhang Z, Hall G, Moreau R, Nunez A, Butrym E, Richards P, Wang S, Cheng Z, Wang C (2004). Fermented beverages of pre-and proto-historic China. National Acad. Sci. 101 (51): 17593-17598.
Crossref

 
 

Michael J, Pelczar J, Chan R, Krieg L (2008). Microbiology. 37th edition Mcgraw hill publishing company limited. New York. pp. 624-626.

 
 

Obri KD (1994). Microbial studies on Corn Dough fermentation. American Association of cereal chemists. J. Cereal Chem. 71(2):186-188).

 
 

Sule EI, Umoh VJ, Whong MZ, Abudullahi IO, Alabi O (2014). Chemical and nutritional value of maize and maize products obtained from selected market in Kaduna state, Nigeria. Afr. J. Food Sci. technol. 5(4):100- 104.

 
 

Takano H, Naito S, Ishida N, Koizumi M, Kano H (2002). Fermentation process and grain structure of baked bread from frozen dough using freez-tolerant yeast. J. Food Sci. 67(7):2725-33.
Crossref

 
 

Vouillamoz JF, McGovern PE, Ergul A, Soylemezoglu G K, Tevzadze G, Meredith CP & Grando MS (2006). Genetic characterization and relationships of traditional grape cultivars from Transcaucasia and Anatolia. Plant Genetic Resources: characterization and utilization. 4 (2):144p.
Crossref

 
 

Walter S (1997). Kanasa Academy of Science. Transaction of the Kanasa academy of Sceince. Method Determination Starch 28:172-174.

 
 

Wang L, Swain E, Hesseltine C (1980). Phytase of molds used in oriental food fermentation. J. Food Sci. 45(5):1262.
Crossref

 
 

World Food Program (2000). Percentage concentration of carbohydrate nutrients in corn grain.

 

 


APA Gudeta, K., & Admassu, M. (2017). Assessment of loss of carbohydrate through fermentation process of yeast (Saccharomyces cerevisiae) from small sample of maize flour dough. African Journal of Food Science, 11(12), 389-396.
Chicago Kasahun Gudeta and Messele Admassu. "Assessment of loss of carbohydrate through fermentation process of yeast (Saccharomyces cerevisiae) from small sample of maize flour dough." African Journal of Food Science 11, no. 12 (2017): 389-396.
MLA Kasahun Gudeta and Messele Admassu. "Assessment of loss of carbohydrate through fermentation process of yeast (Saccharomyces cerevisiae) from small sample of maize flour dough." African Journal of Food Science 11.12 (2017): 389-396.
   
DOI 10.5897/AJFS2017.1628
URL http://academicjournals.org/journal/AJFS/article-abstract/54811B466759

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