African Journal of Food Science
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Article Number - AD40A8366426

Vol.11(11), pp. 369-375 , November 2017
DOI: 10.5897/AJFS2017.1619
ISSN: 1996-0794

Full Length Research Paper

Acute toxicity evaluation of mixture of neem (Azadirachta indica) and moringa (Moringa oleifera) seed oils in rats

Ilesanmi J. O. Y.
  • Ilesanmi J. O. Y.
  • Department of Food Science and Technology, Modibbo Adama University of Technology, Yola, Nigeria.
  • Google Scholar
Gungula D. T.
  • Gungula D. T.
  • Department of Crop Production and Horticulture, Modibbo Adama University of Technology, Yola, Nigeria.
  • Google Scholar
Nadro M. S.
  • Nadro M. S.
  • Department of Biochemistry, Modibbo Adama University of Technology, Yola, Nigeria.
  • Google Scholar

 Received: 23 May 2017  Accepted: 11 September 2017  Published: 30 November 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

The toxicity of the mixture of neem and moringa seed oils in the ratio of 1:3 was evaluated based on some biomarkers of liver and kidney functions of Wistar Albino rats. Thirty male Wistar albino rats were randomly divided into six groups of five rats each. Group A served as control. Groups B, C, D, E and F received doses of 100, 1000, 1600, 2900 and 5000 mg/kg body weight of ratio 1:3 neem-moringa seed oil, respectively. The albino rats were observed for any changes for seven days; during this period, they were allowed free access to food and water ad-libitum. The rats were weighed and made to fast overnight. The serum obtained was used to determine the serum level of alanine transaminase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST). Similarly, liver and kidney tissues were removed and homogenized separately in a normal saline in ratio of 1:10 w/v. The homogenate of liver was centrifuged and the supernatant was used to determine total protein and billirubine while that of kidney was used for determining creatinine and urea. The results of all the biochemical parameters tested did not show any significant difference (P>0.05) from the control up to the dose of 5000 mg/kg body weight and did not produce any visible toxic effect. The dosage of 1:3 mixtures of neem-moringa seed oils appeared to be safe for humans.


Key words: Wistar albino rats, acute toxicity, neem-moringa seed oil, safety, biomarker.

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APA Ilesanmi, J. O. Y., Gungula, D. T., & Nadro, M. S. (2017). Acute toxicity evaluation of mixture of neem (Azadirachta indica) and moringa (Moringa oleifera) seed oils in rats. African Journal of Food Science, 11(11), 369-375.
Chicago Ilesanmi J. O. Y., Gungula D. T. and Nadro M. S.. "Acute toxicity evaluation of mixture of neem (Azadirachta indica) and moringa (Moringa oleifera) seed oils in rats." African Journal of Food Science 11, no. 11 (2017): 369-375.
MLA Ilesanmi J. O. Y., Gungula D. T. and Nadro M. S.. "Acute toxicity evaluation of mixture of neem (Azadirachta indica) and moringa (Moringa oleifera) seed oils in rats." African Journal of Food Science 11.11 (2017): 369-375.
DOI 10.5897/AJFS2017.1619

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