African Journal of Microbiology Research
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Article Number - 81E921C42590


Vol.8(2), pp. 129-137 , January 2014

DOI: 10.5897/AJMR2013.6257   Total Views: 302

ISSN: 1996-0808   Downloaded: 262


Full Length Research Paper

The interactions between esp, fsr, gelE genes and biofilm formation and pfge analysis of clinical Enterococcus faecium strains










 Maryam Diani      Ozlem Gunay Esiyok       M. Nima Ariafar      Fatma Neslihan Yuksel      Evrim Gunes Altuntas      Nefise Akcelik   


Received: 07 August 2013    Accepted: 25 November 2013      Published: 08 January 2014


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Creative Commons Attribution License 4.0 International License




 Abstract

Enterococcus faecium has become an increasingly important nosocomial pathogen due to formation of biofilms on several surfaces. Sixty one (61) E. faecium strains isolated from blood, urine and fecal were assessed for biofilm production, the effect of different glucose concentration on biofilm production and also the presence of esp, fsr and gelE genes. Pulsed field gel electrophoresis (PFGE) method was performed to show chromosomal similarities and also to determine correlation between biofilm formation ability and genetic identity of E. faecium strains. It was observed that glucose concentration of the medium and incubation period can affect biofilm formation of the bacteria. When tested strains were incubated in a medium containing 1% glucose for 48 h, 66.66% of urine isolates, 60.71% fecal isolates and 25% of blood isolates produced strong biofilm structures. esp-positive strains (80% of the all isolates) were also identified as strong biofilm producers compared to esp-negative isolates. As a result of PFGE analyses, isolates numbered 14 (isolated from fecal sample) and 81 (isolated from blood sample) were classified in minor group B at a level of 48% similarity. Out of these two isolates, all the isolates were included in major group A with 43% similarity level and this group was subdivided into six subgroups.

 

Key words: Enterococcus faecium, biofilm, Pulsed Field Gel Electrophoresis (PFGE), esp, fsr, gelE.





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