African Journal of Microbiology Research
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Article Number - 87AF0E843463


Vol.8(9), pp. 929-938 , February 2014
DOI: 10.5897/AJMR2013.6565
ISSN: 1996-0808



Full Length Research Paper

Optimization of phytase production by Aspergillus japonicus Saito URM 5633 using cassava bast as substrate in solid state fermentation



Keila A. Moreira
  • Keila A. Moreira
  • Academic Unit of Garanhuns, Federal Rural University of Pernambuco, CEP 55292-270, Garanhuns, PE, Brazil.
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Polyanna N. Herculano
  • Polyanna N. Herculano
  • Academic Unit of Garanhuns, Federal Rural University of Pernambuco, CEP 55292-270, Garanhuns, PE, Brazil. Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, CEP 52171-900, Recife, PE, Brazil.
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Marília de H. C. Maciel
  • Marília de H. C. Maciel
  • Department of Mycology, Federal University of Pernambuco, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.
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Tatiana S. Porto
  • Tatiana S. Porto
  • Academic Unit of Garanhuns, Federal Rural University of Pernambuco, CEP 55292-270, Garanhuns, PE, Brazil.
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Michele R. Spier
  • Michele R. Spier
  • Bioprocess Engineering and Biotechnology Division, Chemical Engineering Department, Federal University of Paraná, P.O. Box 19011, CEP 81531-970, Curitiba, PR, Brazil.
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Cristina Maria Souza-Motta
  • Cristina Maria Souza-Motta
  • Department of Mycology, Federal University of Pernambuco, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.
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Ana L. F. Porto
  • Ana L. F. Porto
  • Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, CEP 52171-900, Recife, PE, Brazil.
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Carlos R. Soccol
  • Carlos R. Soccol
  • Bioprocess Engineering and Biotechnology Division, Chemical Engineering Department, Federal University of Paraná, P.O. Box 19011, CEP 81531-970, Curitiba, PR, Brazil.
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 Received: 13 December 2013  Accepted: 20 January 2014  Published: 26 February 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Production of phytase by Aspergillus japonicus URM 5633 was investigated by solid state fermentation (SSF) using cassava bast as substrate. The effects of temperature, pH and initial moisture on phytase production were studied using a full factorial design 23. The maximum phytase activity was 174 U/g dry substrate. The optimum conditions for phytase production were pH 6.5 and 40% initial moisture at 26°C with 96 h of fermentation. A factorial design 22 was created to optimise phytase production, where the initial moisture and temperature were the variables studied. The parameters for phytase production were fixed at 30% initial moisture, 30°C and pH 5.5, and the maximum activity obtained was 183.1 U/g dry substrate. The effects of glucose concentration, nitrogen source type and nitrogen source concentration in phytase production was studied using the 23 factorial design. Nitrogen source concentration was significant for phytase activity and had a negative effect, indicating that smaller values increased enzyme activity. The optimum activity for phytase occurred at pH 3.6 and at an apparent optimum temperature of 60°C. Phytase was stable in the pH range of 2.4-3.0 at 30-70°C. The enzymatic activity increased significantly in the presence of FeSO4.

 

Key words: Filamentous fungus, residue, enzyme, production, characterization.

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APA Moreira, K. A., Herculano, P. N., Maciel, M. H. C., Porto, T. S., Spier, M. R., Souza-Motta, C. M., Porto, A. L. F., & Soccol, C. R. (2014). Optimization of phytase production by Aspergillus japonicus Saito URM 5633 using cassava bast as substrate in solid state fermentation. African Journal of Microbiology Research, 8(9), 929-938.
Chicago Keila A. Moreira, Polyanna N. Herculano, Mar&ilia de H. C. Maciel, Tatiana S. Porto, Michele R. Spier, Cristina Maria Souza-Motta, Ana L. F. Porto and Carlos R. Soccol. "Optimization of phytase production by Aspergillus japonicus Saito URM 5633 using cassava bast as substrate in solid state fermentation." African Journal of Microbiology Research 8, no. 9 (2014): 929-938.
MLA Keila A. Moreira, et al. "Optimization of phytase production by Aspergillus japonicus Saito URM 5633 using cassava bast as substrate in solid state fermentation." African Journal of Microbiology Research 8.9 (2014): 929-938.
   
DOI 10.5897/AJMR2013.6565
URL http://academicjournals.org/journal/AJMR/article-abstract/87AF0E843463

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