African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4829

Full Length Research Paper

Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry

C. G. Kouadio-Yapo
  • C. G. Kouadio-Yapo
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar
L. Hasseine
  • L. Hasseine
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar
P. Delaunay
  • P. Delaunay
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar
P. Marty
  • P. Marty
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar
M. Gari-Toussaint
  • M. Gari-Toussaint
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar


  •  Received: 08 December 2017
  •  Accepted: 18 January 2018
  •  Published: 28 January 2018

Abstract

This study aimed to identify yeasts directly from positive blood cultures by MALDI-TOF mass spectrometry. This was a 5-month prospective study (February to June, 2015) carried out on bottles of blood cultures from in-patients at the Hôpital de l’ARCHET at the CHU, Nice. Positive blood culture broth was analysed by MALDI-TOF mass spectrometry using Sodium Dodecyl Sulphate (SDS) as the lysis buffer. Out of 64 samples, 51 (79.6%) were identified by MALDI-TOF mass spectrometry at the species level and 12 strains (18.7%) at the genus level. The isolated yeasts fell into six species: Candida albicans 37.2%, Candida glabrata 31.4%, Candida parapsilosis 15.7%, Candida tropicalis 5.9%, Candida krusei 5.9%, and Candida lusitaniae 3.9%. In comparison, 95.3% of species were identified from cultured colonies, the main ones being C. albicans (37.1%), C. glabrata (30.7%) and C. parapsilosis (16.1%). Identification of yeasts from blood culture bottles by MALDI-TOF mass spectrometry is a fast, reliable technique. However, analysis of colonies remains the best technique for identification and for antifungal imaging in order to refer the patient to the most appropriate therapy.

Key words: Yeasts, blood cultures, identification, MALDI-TOF, mass spectrometry.