African Journal of Microbiology Research
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Article Number - 92944D255832


Vol.12(4), pp. 68-72 , January 2018
https://doi.org/10.5897/AJMR2017.8779
ISSN: 1996-0808


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Full Length Research Paper

Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry



C. G. Kouadio-Yapo
  • C. G. Kouadio-Yapo
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar
L. Hasseine
  • L. Hasseine
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar
P. Delaunay
  • P. Delaunay
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar
P. Marty
  • P. Marty
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar
M. Gari-Toussaint
  • M. Gari-Toussaint
  • Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire l’Archet, CS 23079 06202 Nice Cedex 3, France.
  • Google Scholar







 Received: 08 December 2017  Accepted: 18 January 2018  Published: 28 January 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


This study aimed to identify yeasts directly from positive blood cultures by MALDI-TOF mass spectrometry. This was a 5-month prospective study (February to June, 2015) carried out on bottles of blood cultures from in-patients at the Hôpital de l’ARCHET at the CHU, Nice. Positive blood culture broth was analysed by MALDI-TOF mass spectrometry using Sodium Dodecyl Sulphate (SDS) as the lysis buffer. Out of 64 samples, 51 (79.6%) were identified by MALDI-TOF mass spectrometry at the species level and 12 strains (18.7%) at the genus level. The isolated yeasts fell into six species: Candida albicans 37.2%, Candida glabrata 31.4%, Candida parapsilosis 15.7%, Candida tropicalis 5.9%, Candida krusei 5.9%, and Candida lusitaniae 3.9%. In comparison, 95.3% of species were identified from cultured colonies, the main ones being C. albicans (37.1%), C. glabrata (30.7%) and C. parapsilosis (16.1%). Identification of yeasts from blood culture bottles by MALDI-TOF mass spectrometry is a fast, reliable technique. However, analysis of colonies remains the best technique for identification and for antifungal imaging in order to refer the patient to the most appropriate therapy.

Key words: Yeasts, blood cultures, identification, MALDI-TOF, mass spectrometry.

 

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APA Kouadio-Yapo, C. G., Hasseine, L., Delaunay, P., Marty, P., & Gari-Toussaint, M. (2018). Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry. African Journal of Microbiology Research, 12(4), 68-72.
Chicago C. G. Kouadio-Yapo, L. Hasseine, P. Delaunay, P. Marty and M. Gari-Toussaint  . "Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry." African Journal of Microbiology Research 12, no. 4 (2018): 68-72.
MLA C. G. Kouadio-Yapo, et al. "Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry." African Journal of Microbiology Research 12.4 (2018): 68-72.
   
DOI https://doi.org/10.5897/AJMR2017.8779
URL http://academicjournals.org/journal/AJMR/article-abstract/92944D255832

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