African Journal of Microbiology Research
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Article Number - 92F24AD43148


Vol.8(8), pp. 776-782 , February 2014
DOI: 10.5897/AJMR2013.6576
ISSN: 1996-0808



Full Length Research Paper

Screening novel diagnostic marker of Mycobacterium tuberculosis



Xin Pan
  • Xin Pan
  • Shanghai Junwei Fine Medical Club,58 Bao Tou Road, Shanghai 200433, China. Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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Siliang Zeng
  • Siliang Zeng
  • Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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Jialin Cai
  • Jialin Cai
  • Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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Bin Zhang
  • Bin Zhang
  • Qingpu Branch of Zhongshan Hospital, Fudan University, 1158 Gong Yuan Dong Road, Shanghai 201700, China.
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Boju Pan
  • Boju Pan
  • Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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Zhonglei Pan
  • Zhonglei Pan
  • Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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Ying Wu
  • Ying Wu
  • Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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Ying Wang
  • Ying Wang
  • Shanghai Jiao Tong University School of Medicine, 280 Chongqing South Road, Shanghai, 200025, China.
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Yi Zhou
  • Yi Zhou
  • Shanghai Junwei Fine Medical Club,58 Bao Tou Road, Shanghai 200433, China.
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Wei Fang
  • Wei Fang
  • Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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Min Chen
  • Min Chen
  • Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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Wanqing Liao
  • Wanqing Liao
  • Second Military Medical University,800 Xiang Yin Road, Shanghai, 200433, China.
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XiuZhen Yu
  • XiuZhen Yu
  • Shanghai Junwei Fine Medical Club,58 Bao Tou Road, Shanghai 200433, China.
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Min Tao
  • Min Tao
  • Shanghai Junwei Fine Medical Club,58 Bao Tou Road, Shanghai 200433, China.
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Jun Zhang
  • Jun Zhang
  • Shanghai Junwei Fine Medical Club,58 Bao Tou Road, Shanghai 200433, China.
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Wei Song
  • Wei Song
  • Shanghai Junwei Fine Medical Club,58 Bao Tou Road, Shanghai 200433, China.
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 Accepted: 23 January 2014  Published: 19 February 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The aim of present study was to screen and evaluate the serodiagnostic value of the purified fusion antigens of Mycobacterium tuberculosis (Mtb) by enzyme-linked immunosorbent assay (ELISA). A group of vector system which was constructed by cloning Rv1908c, Rv0733, Rv0899, Rv1411c and Rv3914 gene of Mtb into the prokaryotic expression plasmid pET-32b was transformed into E. coli for induction and expression fusion antigens to determine their potentiality in diagnostic application. Following purification with the His-select nickel magnetic agarose beads, the expressed fusion proteins showed purity via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot technique. The ELISA plates were coated with these fusion antigens. Through ELISA, the antigen binding with specific IgG levels between active TB patients and healthy controls was compared. The purity and the size of the expressed fusion antigens were confirmed by the Western blot. The ELISA results indicated that IgG levels against Rv1411c-6His, Rv3914-6His and Rv2031c-6His were significantly higher in serum of active TB patients than in that of healthy controls. More interesting, the AUC value of Rv3914-6His (0.7867) was higher than that of Rv2031c-6His (0.754) which was widely used in clinic. The results implied that Rv3914-6His might be a useful candidate antigen in the diagnosis of Mtb infection, especially for active pulmonary TB diagnosis. Its role in serodiagnosis of extra-pulmonary TB is still needed to be validated.

 

Key words: Mycobacterium tuberculosis, expression and purification, antigen, serodiagnosis, tuberculosis

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APA (2014). Screening novel diagnostic marker of Mycobacterium tuberculosis. African Journal of Microbiology Research, 8(8), 776-782.
Chicago Xin Pan, Siliang Zeng, Jialin Cai, Bin Zhang, Boju Pan, Zhonglei Pan, Ying Wu, Ying Wang, Yi Zhou, Wei Fang, Min Chen, Wanqing Liao, XiuZhen Yu, Min Tao, Jun Zhang and Wei Song. "Screening novel diagnostic marker of Mycobacterium tuberculosis." African Journal of Microbiology Research 8, no. 8 (2014): 776-782.
MLA Xin Pan, et al. "Screening novel diagnostic marker of Mycobacterium tuberculosis." African Journal of Microbiology Research 8.8 (2014): 776-782.
   
DOI 10.5897/AJMR2013.6576
URL http://academicjournals.org/journal/AJMR/article-abstract/92F24AD43148

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