African Journal of Microbiology Research
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Article Number - A8C15F413333


Vol.7(31), pp. 4001-4005 , August 2013
DOI: 10.5897/AJMR2013.5748
ISSN: 1996-0808



Full Length Research Paper

Development of an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) to detect and genotype enterotoxigenic Escherichia coli of calf origin


 

Xiu-Yan LANG, Yang-Long ZHANG, Hui-Ting JIANG, Jiao LIU and Hong-Bo NI*




College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, P.R. China.


Email: nihongbo@sina.com






 Accepted: 03 June 2013  Published: 02 August 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

We develop an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) DNA fingerprinting technique for genotyping enterotoxigenic Escherichia coli(ETEC) in calves. Using the ERIC-PCR, we detected 66 ETEC strains in calves isolated from 11 farms. The ETEC strains could be grouped into 17 types, with type D predominant. The ERIC-PCR system is an efficient method for identification, typing and tracking of ETEC. Serotyping using a specific O antigen revealed 15 serotypes, with serotype O111 most prevalent (21.2%). We were unable to serotype 21.5% of the ETEC strains we had previously identified by ERIC-PCR. Our results indicate that some ETEC strains had identical genotypes, however their serotype might differ and vice versa. The ERIC-PCR method is rapid, sensitive, repeatable and reliable, and we believe it should be applied for the genotyping of ETEC in calves.

 

Key Words: Calf, enterotoxigenic Escherichia coli, ERIC-PCR, genotyping, serotyping.


APA (2013). Development of an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) to detect and genotype enterotoxigenic Escherichia coli of calf origin. African Journal of Microbiology Research, 7(31), 4001-4005.
Chicago   Xiu-Yan LANG, Yang-Long ZHANG, Hui-Ting JIANG, Jiao LIU and Hong-Bo NI. "Development of an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) to detect and genotype enterotoxigenic Escherichia coli of calf origin." African Journal of Microbiology Research 7, no. 31 (2013): 4001-4005.
MLA Xiu-Yan LANG, et al. "Development of an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) to detect and genotype enterotoxigenic Escherichia coli of calf origin." African Journal of Microbiology Research 7.31 (2013): 4001-4005.
   
DOI 10.5897/AJMR2013.5748
URL http://academicjournals.org/journal/AJMR/article-abstract/A8C15F413333

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