African Journal of Microbiology Research
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Article Number - B7A605862345

Vol.11(2), pp. 55-64 , January 2017
ISSN: 1996-0808

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Full Length Research Paper

Bacterial populations of mosquito breeding habitats in relation to maize pollen in Asendabo, south western Ethiopia

Eyob Chukalo*
  • Eyob Chukalo*
  • Addis Ababa University, Natural Science Faculty, Ethiopia.
  • Google Scholar
Dawit Abate
  • Dawit Abate
  • Addis Ababa University, Natural Science Faculty, Ethiopia.
  • Google Scholar

 Received: 31 August 2016  Accepted: 26 October 2016  Published: 14 January 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

Mosquito larvae feed on particulate organic matter including microorganisms. This study was conducted to investigate the diversity and abundant bacteria of Anopheles mosquito larva breeding habitats and to evaluate the contribution of maize pollen as source of nutrients for bacterial growth. The nutrient composition (COD, NH3-N and TP) of the larva breeding habitats water samples were measured by HACH 2010. Bacteria were isolated and enumerated from 18 water samples of larva habitats. The abundance of bacteria in the larva breeding habitats was significantly different. Bacteria were found to be grown abundant in habitats close to maize pollen sources (tasseled zone). This implies that maize pollen contributes to bacterial abundance. The Pearson Correlation showed that there was positive relationship between bacterial abundance and physicochemical characteristics of the water samples. The bacterial population in the habitat was dominated by species of Bacillus, Pseudomonas, Micrococcus and Serratia.  The dominant bacteria were tested for their capability to grow on maize pollen medium. The growth kinetics of bacteria on maize pollen broth was performed to 18 h culture using JENWAY spectrophotometer at 600 nm wave length. The bacteria could show optimum growth on Maize Pollen broth at 15 g/l as of nutrient broth. The release of maize pollen during anthesis in rainy season in habitats close to larva breeding pool and its nutriment quality support proliferation of large array of bacteria which results in increased larval nourishment. Increased malaria transmission in Asendabo could thus be caused as the bacteria serve as source of nutrients for mosquito larva.

Key words: Maize pollen, microbial flora, mosquito larva habitat, maize pollen broth, bacterial abundance.

Abdulrahaman A, Kolawole AOM (2006). Traditional preparations and uses of maize in Nigeria. Ethnobotanical Leaflets 10:219-227.


Ayele AA, Gebre-Michael T, Balkew M, Lindtjørn B (2012). Abundance and dynamics of anopheline larvae in a highland malarious area of south-central Ethiopia. Parasites & Vectors 5(117):1-9


Adugna N, Melaku A (2001). Hybrid maize seed production and commercialization: The experience of pioneer hybrid seeds in Ethiopia. Second national maize workshop of Ethiopia. Pioneer Hybrid Seeds PLC, Addis Ababa.


Afrane A, Lawson BW, Brenya R, Kruppa T, Yan G (2012). The ecology of mosquitoes in an irrigated vegetable farm in Kumasi, Ghana: abundance, productivity and survivorship. Parasit. Vectors 5(233):1-7.


Aneja KR (2005). Experiments in Microbiolo-gy, Plantpathology and Biotechnology, 4th edn. New Age International PLtd. Publishers, New Delhi pp. 245-275.


APHA (1998). Standard Methods for the Examination of Water and Waste Water, 20th edn. American Public Health Association, Wshington, DC.


Barros FS, Arruda ME, Gurgel HC, Honório NA (2011). Spatial clustering and longitudinal variation of Anopheles darlingi (Diptera:Culicidae) larvae in a river of the Amazon:the importance of the forest fringe and of obstructions to flow in frontier malaria. Cambridge University Press, Bullet. Entomol. Res. 101:643-658.


Bergey's Manual of Systematic Bacteriology (1984). Eds. Krieg, N.R. and Holt, J.G. Williams and Wilkins, Baltimore, USA. Vol 1.


Berhanu G, Fernandez-Rivera S, Mo-hammed H, Mwangi W, Seid A (2007). Maize and livestock: their inter-linked roles in meeting human needs in Ethiopia, Pp.1-87. Research report 6 .ILRI, Nirobi.


Bezawit E (2007). Nutrient Composition of Maize Pollen and its Microbial Degradation. M. Sc. Thesis, Addis Ababa University, Addis Ababa.


Bisen PS, Verma K (1994). Hand Book of Microbiology. CBS Publishers, New Delhi, pp. 47-65.


Chester B (1979). Semi quantitative catalase test as an aid in identification of oxidative and non saccharolytic gramnegative bacteria. J. Clin. Microbiol. 10:525-528.


EEPA (2002). General standards for all other industrial effluents: effluent discharges to inland water. Ethiopian Environment Protection Authority, Addis Ababa, pp. 20-23.


Fry JC, Zia T (1982). Viability of heterotrophic bacteria in freshwater. J. General Microbiol. 128:2841-2850.


Garros C, Ngugi N, Githeko AE, Tuno N, Yang G (2008). Gut content identification of larvae of the Anopheles gambiae complex in Western Kenya using a barcoding approach. Mol. Ecol. Res. 8:512-518.


Getahun D, Mwangi W, Verkuijl H, Abdishekur W (2000). An assessment of the adoption of seed and fertilizer packages and the role of credit in smallholder maize production in Sidama and North Omo Zone, Ethiopia. Mexico, D.F.: International Maize and Wheat Improvement Center (CIMMYT) and Ethiopian Agricultural Research Organization (EARO), pp. 12-22.


Herrel N, Amerasinghe FP, Ensink J, Mukhtar M, Vanderhoek W, Konradsen F (2001). Breeding of anopheles mosquitoes in irrigated areas of South Punjab, Pakistan. Blackwell Science Ltd, Med. Veterinary Entomol. 15:236-248.


Imam AA, Deeni Y (2014). Common Types of Anopheles gambiae Breeding Habitats in North Western Nigeria. J. Innov. Res. Eng. Sci. 4(4):496-504.


Kaufman MG, Walker ED, Smith TW, Merritt RW, Klug MJ (1999). Effects of larval mosquitoes (Aedes triseriatus) and stem flow on microbial community dynamics in container habitats. Appl. Environ. Microbiol. 65:2661-2673.


Kettle DS (1995). Medical and Veterinary Entomology, 2nd edn. CABI Publisher pp. 109-151.


Kling LJ, Juliano SA, Yee DA (2007). Larval mosquito communities in discarded vehicle tires in a forested and unforested site: detritus type, amount, and water nutrient differences. J. Vector Ecol. 32:207-217.


Kudom AA (2015). Larval ecology of Anopheles coluzzii in Cape Coast, Ghana: water quality, nature of habitat and implication for larval control. Malaria J. 14(447):1-13.


Le J, Wehr JD Campbell L (1994). Uncoupling of bacterioplankton and phytoplankton production in fresh Waters is affected by inorganic nutrient limitation. Appl. Environ. Microbiol. 60:2086-2093.


Machault V, Gadiaga L, Vignolles C, Jarja-val F, Bouzid S, Sokhna C, Lacaux, J, Trape J, Rogier C Pages F (2009). Highly focused Anopheline breeding sites and malaria transmission in Dakar. Malaria J. 8:138.


Maekawa E, Aonuma H, Nelson B, Yoshimura A, Tokunaga F, Fukumoto S, Kanuka H (2011). The role of proboscis of the malaria vector mosquito Anopheles stephensis in host seeking behavior. Parasites Vectors 4(1):1-10.


Mala AO, Irungu LW (2011). Factors influencing differential larval habitat productivity of Anopheles gambiae complex mosquitoes in a western Kenyan village. J. Vector Borne Dis. 48:52-57.


Merritt RW, Dadd RH, Walker ED (1992). Feeding behavior, natural food, and nutritional relationships of larval mosquitoes. Ann. Rev. Entomol. 37:349-376.


Mondal R, Devi NP, Jauhari RK (2015). Bacterial characterization in natural breeding habitats of Aedes mosquitoes and their role on ovipositional response. Int. J. Mosq. Res. 2(3):175-181.


Muirhead-Thomson RC (1958). The ecology of vector snail habitats and mosquito breeding- places: The experimental approach to basic problems. Bull. org. Mond. Sante 19:637-659.


Muturi EJ, Orindi BO, Kim CH (2013). Effect of Leaf Type and Pesticide Exposure on Abundance of Bacterial Taxa in Mosquito Larval Habitats. PLoS/ONE 8(8):1-8.


Mwangangi JM, Mbogo CM, Muturi EJ, Nzovu JG, Githure JI, Yan G, Minakawa N, Novak R, Beier JC (2007). Spatial distribution and habitat characterization of Anopheles larvae along the Kenyan coast. J. Vect. Borne Dis. 44:44-51.


Okech BA, Gouagna LC, Yan G, Githure JI, Beier JC (2007). Larval habitats of Anopheles gambiae s.s. (Diptera: Culicidae) influences vector competence to plasmodium falciparum parasites. Malaria J. 6:50.


Okogun GRA, Nwoke BEB, Okere ANJ, Anosike JC, Esekhegbe AC (2003). Epidemiological implications of preferences of breeding sites of mosquito species in mid-western Nigeria. Ann. Agric. Environ. Med. 10:217-222.


Pereira EDS, Sarquis MID, Ferriera-Keppler RL, Hamada N, Alencar Y (2009). Filamentous fungi associated with mosquito larvae (Dipteria: Culicidae) in municipalities of the Brazilian Amazon. Neotrop. Entomol. 38:352-359.


Pfaehler O, Oulo DO, Guoagna LC, Githure J, Guerin PM (2006). Influence of soil quality in the larval habitat on development of Anopheles gambie giles. J. Vector Ecol. 31:400-405.


Phillips S (1995). Poaceae (Gramineae). In: Flora of Ethiopia and Eritrea, 7:365-368 (Hedberg, I. and Edwards S, eds). Addis Ababa and Uppsala.


Rediat A (2008). Seasonal Studies on Phyto-plankton in Relation to some Biological and Physicochemical Factors in lake HoraKilole, Ethiopia. M. Sc. Thesis, Addis Ababa University, Addis Ababa.


Schindler D (2001). The cumulative effects of climate warming and other human stresses on Canadian freshwaters in the new millennium. Can. J. Fish. Aquat. Sci. 58:18-29.


Swift DR (1981). Preliminary investigations of periphyton and water quality relation-ships in the everglades water conservation areas. South Florida Water Management District Technical Publication 81-5. West Palm Beach, Florida, 83 p.


Tchioffo MT, Boissie`re A, Churcher TS, Abate L, Gimonneau G, Nsango SE, Awono-Ambe’ne´ PH, Christen R, Berry A, Morlais I (2013). Modulation of Malaria Infection in Anopheles gambiae Mosquitoes Exposed to Natural Midgut Bacteria. PLoS ONE 8(12):1-9.


Wotton RS, Chaloner DT, Yardley TCA, Merrittt RW (1997). Growth of Anopheles mosquito larvae on dietary microbiota in aquatic surface microlayers. Med. Vet. Entomol. 11:65-70.


Yemane Ye-ebiyo, Pollack RJ, Spielman A (2000). Enhanced development in nature of larval Anopheles arabiensis mosquitoes feeding on maize pollen. Am.J.Med.Hyg.63: 90-93.


Yemane Ye-ebiyo, Pollack RJ, Kiszewski A, Spielman A (2003a). Enhancement of development of larval Anopheles arabiensis by proximity to flowering maize (Zea mays) in turbid water and when crowded. Am. J. Trop. Med. Hyg. 68:748-752.


Yemane Ye-ebiyo, Pollack RJ, Kiszewski A, Spielman A (2003b). A component of maize pollen that stimulates larval mosquitoes (Dipteria: Culicidae) to feed and increases toxicity of microbial larvicides. J. Med. Entomol. 40:860-864.


APA Chukalo, E., & Abate, D. (2017). Bacterial populations of mosquito breeding habitats in relation to maize pollen in Asendabo, south western Ethiopia. African Journal of Microbiology Research, 11(2), 55-64.
Chicago Eyob Chukalo and Dawit Abate. "Bacterial populations of mosquito breeding habitats in relation to maize pollen in Asendabo, south western Ethiopia." African Journal of Microbiology Research 11, no. 2 (2017): 55-64.
MLA Eyob Chukalo and Dawit Abate. "Bacterial populations of mosquito breeding habitats in relation to maize pollen in Asendabo, south western Ethiopia." African Journal of Microbiology Research 11.2 (2017): 55-64.

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