October 2013
Hemin transported protein of Xanthomonas axonopodis pv. glycines functions on leaf colonization and virulence on soybean
Xanthomonas axonopodis pv. glycines (Xag) causes bacterial pustule disease on soybean. This bacterium is present worldwide around hot and humid growing regions such as Southeast Asia. To understand if the gene coding for hemin transport protein (hem) is involved in virulence of the pathogen in soybean, we generated a hem mutant in Xag by overlapping PCR mutagenesis. Disruption...
October 2013
Detection of antibiotic resistant Enterobacteriaceae from dogs in North West University (South Africa) animal health hospital
Bacteria belonging to the family Enterobacteriaceae are facultative anaerobic, Gram-negative, non-spore forming rod-shaped bacilli. Members of this heterogeneous group of bacteria do not only form part of the normal flora of humans and animals, but are also widely distributed in various environments such as water, soil and plants. Most members of the Enterobacteriaceae were previously considered to...
October 2013
Antimicrobial activity of five plants from Northern Mexico on medically important bacteria
The aim of this study was to evaluate the potential antimicrobial activity of five medicinal plants from Northern Mexico against ATCC bacteria Klebsiella pneumoniae (9183),Staphylococcus aureus (BAA44), Escherichia coli (O157), Enterobacter aerogenes(9180) and Enterobacter cloacae (9235) and eight clinical isolated strains (CI) Klebsiella pneumoniae, Staphylococcus...
October 2013
Efficient cellulase production from low-cost substrates by Trichoderma reesei and its application on the enzymatic hydrolysis of corncob
In this work, cellulase production by Trichoderma reesei CH11 using different low-cost raw materials as carbon sources and its application on the enzymatic hydrolysis of corncob were systematically investigated. Among all the studied carbon sources, rice straw was the most suitable one for T. reesei to produce cellulolytic enzymes. In addition, adding wheat bran and ammonium sulfate improved the...
October 2013
Optimization of bioplastic (poly-β-hydroxybutyrate) production by a promising Azomonas macrocytogenes bacterial isolate P173
An extensive screening program was previously done to isolate a promising bacterial isolate Azomonas macrocytogenes isolate P173 capable of polyhydroxybutyrate (PHB) production. It produced 24% PHB per dry weight after 48 h. In this study, several experiments were designed to optimize the composition of the culture medium and environmental factors for maximizing PHB production by the respective isolate....
October 2013
Mycoflora associated with processed and stored cassava chips in rural areas of southern Cameroon
A total of 132 home-stored samples of cassava chips were collected in 45 villages situated in three distinct geographical locations of Southern Cameroon for assessment of their mycoflora. Cassava chips were split into small pieces (10 x 10 x 5 mm) and these were plated onto Petri dishes containing water agar at 25°C after a 7 day incubation period to yield of mycoflora. Aspergillus, Fusarium and Penicillium spp....
October 2013
Prevalence and antimicrobial resistance profile of Salmonella isolates from dairy products in Addis Ababa, Ethiopia
A cross sectional study was conducted on dairy items in Addis Ababa from October 2010 to March 2011 to determine prevalence and antimicrobial resistance profile of Salmonella. A total of 384 dairy items, 96 of each item (cheese, milk, butter and yogurt) was sampled. The overall prevalence of Salmonella was found to be 1.6% (6 of 384). Prevalence of 3.1, 1.04, 2.1, and 0% was observed from cheese, butter,...
October 2013
Study on prevalence of bovine mastitis in lactating cows and associated risk factors in and around Areka town, Southern of Ethiopia
A cross-sectional study was conducted from October 2011 to March 2012 on a total of 384 lactating (Zebu 183, Jersey103 and Holstein 93) dairy cows randomly selected from 29 rural kebele in which three small scale dairy farms were included. The study was designed with the objective to determine the prevalence of mastitis and isolate the major bacteria that causes mastitis involved in areka woreda and its surrounding...
October 2013
Morphological, ultrastructural and molecular variability studies of wild and mutant strains of edible Pleurotus species using growth yield, scanning electron microscopy and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)
There is a growing industry of edible mushroom production due to their nutritive value and the recognized fact that mushrooms are natural and healthy foods originating from an environmentally friendly organic farming system. The production of edible mushrooms is threatened by both abiotic and biotic factors, hence the need to improve breeding through genetic tools. In an attempt to determine the morphological and...
October 2013
The Effect of 50 Hz electromagnetic field on Helicobacter pylori
We aimed to determine the impact of 50 Hz, 3 mT electromagnetic field (EMF) on the growth of Helicobacter pylori and the changes in morphology, survival and viability after exposure in both 20 and 120 min of in vitro experiments. The bacterial suspension (~108CFU/mL) of cultured H. pylori NCTC 11637 in Brucella Broth were exposed to 50 Hz EMF for 20 and 120 min in a microaerophilic...
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