African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2092

Full Length Research Paper

Evaluation of antioxidant capacity, phenolic content, antimicrobial and cytotoxic properties of Guettarda uruguensis flowers and fruits

Ana Flávia Schvabe Duarte
  • Ana Flávia Schvabe Duarte
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Ellis Marina Szabo
  • Ellis Marina Szabo
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Isabel Schvabe Duarte
  • Isabel Schvabe Duarte
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Francis José Zortéa Merino
  • Francis José Zortéa Merino
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Vinícius Bednarczuk de Oliveira
  • Vinícius Bednarczuk de Oliveira
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Marilis Dallarmi Miguel
  • Marilis Dallarmi Miguel
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Obdulio Gomes Miguel
  • Obdulio Gomes Miguel
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar


  •  Received: 16 October 2016
  •  Accepted: 28 November 2016
  •  Published: 22 December 2016

Abstract

Antioxidant capacity, phenolic content, antimicrobial and cytotoxic properties of extracts and fractions from flowers and fruits of G. uruguensis were evaluated. Total phenolic content ranged between 406.8 ± 20.47 and 57.98 ± 1.734 gallic acid equivalent in mg g-1 (mg GAE g-1) samples. Ethyl acetate and butanol fractions from flowers had the greatest rates in phenolic content and antioxidant activity in 1,1-diphenyl-2-picrylhydrazyl (DPPH) and thiobarbituric acid reactive substances (TBARS) assays. Pearson´s coefficient showed a strong association between total phenolic compounds in DPPH (r = - 0.83, p < 0.01) and TBARS (r = 0.85, p < 0.01). Eleven out of the 12 samples tested were toxic in the brine shrimp assay (LC50 < 100 µg mL-1). Antimicrobial susceptibility profile revealed flower remaining fraction and fruit extract with moderate activity against Enterococcus faecalis. Results indicate that G. uruguensis is a good source of compounds with antioxidant and cytotoxic activities. Further studies to identify compounds causing these activities are recommended.

Key words: Guettarda uruguensis, total phenolics, antioxidant activity, antimicrobial, brine shrimp lethality.