African Journal of Pharmacy and Pharmacology
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Article Number - 4CC37A362172


Vol.10(47), pp. 1014-1024 , December 2016
DOI: 10.5897/AJPP2016.4684
ISSN: 1996-0816



Full Length Research Paper

Evaluation of antioxidant capacity, phenolic content, antimicrobial and cytotoxic properties of Guettarda uruguensis flowers and fruits



Ana Flávia Schvabe Duarte
  • Ana Flávia Schvabe Duarte
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Ellis Marina Szabo
  • Ellis Marina Szabo
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Isabel Schvabe Duarte
  • Isabel Schvabe Duarte
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Francis José Zortéa Merino
  • Francis José Zortéa Merino
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Vinícius Bednarczuk de Oliveira
  • Vinícius Bednarczuk de Oliveira
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Marilis Dallarmi Miguel
  • Marilis Dallarmi Miguel
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar
Obdulio Gomes Miguel
  • Obdulio Gomes Miguel
  • Department of Pharmacy, Federal University of Paraná, 80210-170, Curitiba PR Brazil.
  • Google Scholar







 Received: 16 October 2016  Accepted: 28 November 2016  Published: 22 December 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Antioxidant capacity, phenolic content, antimicrobial and cytotoxic properties of extracts and fractions from flowers and fruits of G. uruguensis were evaluated. Total phenolic content ranged between 406.8 ± 20.47 and 57.98 ± 1.734 gallic acid equivalent in mg g-1 (mg GAE g-1) samples. Ethyl acetate and butanol fractions from flowers had the greatest rates in phenolic content and antioxidant activity in 1,1-diphenyl-2-picrylhydrazyl (DPPH) and thiobarbituric acid reactive substances (TBARS) assays. Pearson´s coefficient showed a strong association between total phenolic compounds in DPPH (r = - 0.83, p < 0.01) and TBARS (r = 0.85, p < 0.01). Eleven out of the 12 samples tested were toxic in the brine shrimp assay (LC50 < 100 µg mL-1). Antimicrobial susceptibility profile revealed flower remaining fraction and fruit extract with moderate activity against Enterococcus faecalis. Results indicate that G. uruguensis is a good source of compounds with antioxidant and cytotoxic activities. Further studies to identify compounds causing these activities are recommended.

Key words: Guettarda uruguensis, total phenolics, antioxidant activity, antimicrobial, brine shrimp lethality.

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APA Duarte, A. F. S., Szabo, E. M., Duarte, I. S., Merino, F. J. Z., de Oliveira, V. B., Miguel, M. D., & Miguel, O. G. (2016). Evaluation of antioxidant capacity, phenolic content, antimicrobial and cytotoxic properties of Guettarda uruguensis flowers and fruits. African Journal of Pharmacy and Pharmacology, 10(47), 1014-1024.
Chicago Ana Fl&avia Schvabe Duarte, Ellis Marina Szabo, Isabel Schvabe Duarte, Francis Jos&e Zort&ea Merino, Vin&icius Bednarczuk de Oliveira, Marilis Dallarmi Miguel and Obdulio Gomes Miguel. "Evaluation of antioxidant capacity, phenolic content, antimicrobial and cytotoxic properties of Guettarda uruguensis flowers and fruits." African Journal of Pharmacy and Pharmacology 10, no. 47 (2016): 1014-1024.
MLA Ana Flaacute;via Schvabe Duarte, et al. "Evaluation of antioxidant capacity, phenolic content, antimicrobial and cytotoxic properties of Guettarda uruguensis flowers and fruits." African Journal of Pharmacy and Pharmacology 10.47 (2016): 1014-1024.
   
DOI 10.5897/AJPP2016.4684
URL http://academicjournals.org/journal/AJPP/article-abstract/4CC37A362172

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