African Journal of Pharmacy and Pharmacology
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Article Number - 893C7BD43059


Vol.8(5), pp. 136-147 , February 2014
DOI: 10.5897/AJPP2013.3836
ISSN: 1996-0816



Full Length Research Paper

Evaluation of antioxidant capacity of the aqueous extract of Cynara scolymus L. (Asteraceae) in vitro and in Saccharomyces cerevisiae



George Laylson da Silva Oliveira
  • George Laylson da Silva Oliveira
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
  • Google Scholar
Francisco Rodrigo de Asevedo Mendes de Oliveira
  • Francisco Rodrigo de Asevedo Mendes de Oliveira
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
  • Google Scholar
Marcus Vinícius Oliveira Barros de Alencar
  • Marcus Vinícius Oliveira Barros de Alencar
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
  • Google Scholar
Antonio Luiz Gomes Junior
  • Antonio Luiz Gomes Junior
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
  • Google Scholar
Alexandre Araujo de Souza
  • Alexandre Araujo de Souza
  • Federal University of Piauí, CCN, Chemistry Department, Ininga, Teresina, PI, 64048-901, Brazil.
  • Google Scholar
Ana Amélia de Carvalho Melo Cavalcante
  • Ana Amélia de Carvalho Melo Cavalcante
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
  • Google Scholar
Rivelilson Mendes de Freitas
  • Rivelilson Mendes de Freitas
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
  • Google Scholar







 Accepted: 03 February 2014  Published: 08 February 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The present study evaluated the antioxidant capacity of the aqueous extract of the leaves of Cynara scolymus on Saccharomyces cerevisiae strains, proficient and deficient in antioxidant defenses, and by in vitro methods with 1,1-diphenyl-2-picrylhydrazyl (DPPH•), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+), inhibition of hydrogen peroxide, lipid peroxidation, formation of nitric oxide, and removal of the hydroxyl radical. A simultaneous quantitative analysis to total phenolics, flavonoids and hydrolysable tannin was also performed, and according to the results obtained, phenolic compounds (661.15 ± 20.11 mg GAE/g of E), flavonoids (123.96 ± 11.47 mg R/g of E) and hydrolysable tannin (14.25 ± 3.18 mg GAE/g of E) were detected in the aqueous extract of C. scolymus. The aqueous extract at study showed high antioxidant capacity in all antioxidant methods in vitro and exhibited significant antioxidant capacity to protect strains of S. cerevisiae from oxidative damage induced by hydrogen peroxide. The analysis of the correlation made between the content of phenolic compounds and the different antioxidants in vitro methods, indicated that these compounds are mainly responsible for the antioxidant capacity of the aqueous extract of C. scolymus. Therefore, this study suggests that the aqueous extract of leaves of C. scolymus is a great natural source of compounds with antioxidant capacity.

Key words: Antioxidant capacity, Cynara scolymus, Saccharomyces cerevisiae, phenolic compounds.

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APA Oliveira, G.L.S., Oliveira, F.R.A. M., de Alencar, M.V.O.B., Junior, A.L.G., de Souza, A.A., Cavalcante, A.A.C.M., & de Freitas, R.M. (2014). Evaluation of antioxidant capacity of the aqueous extract of Cynara scolymus L. (Asteraceae) in vitro and in Saccharomyces cerevisiae. African Journal of Pharmacy and Pharmacology, 8(5), 136-147.
Chicago George Laylson da Silva Oliveira, Francisco Rodrigo de Asevedo Mendes de Oliveira, Marcus Vin&icius Oliveira Barros de Alencar, Antonio Luiz Gomes Junior, Alexandre Araujo de Souza, Ana Am&elia de Carvalho Melo Cavalcante and Rivelilson Mendes de Freitas. "Evaluation of antioxidant capacity of the aqueous extract of Cynara scolymus L. (Asteraceae) in vitro and in Saccharomyces cerevisiae." African Journal of Pharmacy and Pharmacology 8, no. 5 (2014): 136-147.
MLA George Laylson da Silva Oliveira, et al. "Evaluation of antioxidant capacity of the aqueous extract of Cynara scolymus L. (Asteraceae) in vitro and in Saccharomyces cerevisiae." African Journal of Pharmacy and Pharmacology 8.5 (2014): 136-147.
   
DOI 10.5897/AJPP2013.3836
URL http://academicjournals.org/journal/AJPP/article-abstract/893C7BD43059

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