African Journal of Plant Science
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Article Number - C14A2BD66937

Vol.12(1), pp. 7-16 , January 2018
DOI: 10.5897/AJPS2017.1547
ISSN: 1996-0824

Full Length Research Paper

Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)

Osagie-Eweka, S. D. E.
  • Osagie-Eweka, S. D. E.
  • Department of Biochemistry, Faculty of Life Sciences, University of Benin, P. M. B. 1154, Benin City, Edo State, Nigeria.
  • Google Scholar

 Received: 15 March 2017  Accepted: 31 July 2017  Published: 31 January 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

This study was conducted to evaluate selected phytochemicals (antioxidant potentials of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca) relative to standard antioxidants. Sample was harvested, air dried, pulverized and extracted with aqueous and absolute methanol and ethanol; freeze dried at the National Energy Commission Centre, University of Benin. Alkaloids, phenols and tannins were identified, and also flavonoid was detected. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power activity, total antioxidant activity, hydroxyl radical activity, trolox equivalent antioxidant activity and nitric oxide (NO•) radical scavenging activity of stem bark extracts of S. glauca were evaluated, and all the experiment were conducted in a dose dependent manner. Butylated hydroxytuolene (BHT), ascorbate and trolox were introduced as positive controls (antioxidant). DPPH radical scavenging activity of stem bark extracts did not exhibit anti-radical activity at 50% inhibition but demonstrated less anti-radical activity at percentage inhibition lower than 50%. Extracts yielded significant reducing power and total antioxidant activities (FRAP). Hydroxyl radical scavenging activities of extracts was substantial; extracts exhibited significant anti-radical activity when trolox was introduced as positive antioxidant control, while nitric oxide scavenging activities was unprecedented. The presence of phytochemicals and antioxidant principles proffer high medicinal value to S. glauca.
Key words: Simarouba glauca, stem bark, oxidants, radical scavenging properties.

Benzie IFF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal. Biochem. 239:70-76.


Biswas PK (2007). Personal communication Drought tolerant tree for water shed, The Hindu.


Boeing JS, Barizão EO, Costa e Silva B, Montanher PF, Almeida VC, Visentainer JV (2014). Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. J. Chem. Cent. 8:48.


Brand-Williams W, Cuvelier ME, Berset C (1995). Use of a free radical method to evaluate antioxidant activity. J. Food. Sci. Technol. 28(1):25-30.


Chung K, Yee Wong T, Wei C, Huang Y, Lin Y (1998). Tannins and Human Health: A review. Crit. Rev. Food. Sci. Nutr. 38(6):421-464.


Deepa HR, Nalini MS (2013). Phytochemical Screening, Total Phenolic Content and in vitro antioxidant studies of Leaf, Bark and Flower extracts of Schefflera Spp. (Araliaceae). J. Appl. Pharm. Sci. 3(11):94-98.


Demiray S, Pintado ME, Castro PML (2009). Evaluation of phenolic profiles and antioxidant activities of Turkish medicinal plants: Tilia argentea, Crataegi folium leaves and Polygonum bistorta roots. World Acad. Sci. Eng. Technol. 54:312-317.


Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR (2008). Iron chelating activity screening, phenol and flavonoid content of some medicinal plants from Iran. Afr. J. Biotechnol. 32:43-49.


Ferreira ICFR, Baptista M, Vilas-Boas, Barros L (2007). Free radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual caps and stipes activity. Food Chem. 100:1511-1516.


Garratt DC (1964). The quantitative analysis of drugs. Chapman and Hall. 2nd (ed) P 234.


Gulcin I, Huyut Z, Elmastas M, Hassan Y, Aboul-Enein (2010). Radical scavenging and antioxidant activity of tannic acid. Arab. J. Chem. 3(1):43-53.


Harsh M (2010). Textbook of Pathology. 6th (ed). Jaypee Brothers medical publishers, New Delhi, India, pp. 21-34.


Kalow W, Grant DM (1995). Pharmacogenetics In: the metabolic and molecular base of inherited disease. McGraw-Hill. 7th (ed). pp. 345-349.


Kaur S, Mondal P (2014). Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. J. Microbiol. Exp. 1(1):1-6.


Kumar S, Kumar D (2009). Antioxidant and free radical scavenging activities of edible weeds. Afr. J. Food. Agric. Nutr. Dev. 9:1174-1190.


Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD (2007). Screening seeds of some Scottish plants for free-radical scavenging activity. Phytother. Res. 21(7):615-621.


Lakshmi KS, Sangeetha D, Sivamani S, Tamilarasan M, Rajesh TP, Anandraj B (2014). In vitro Antibacterial, Antioxidant, Haemolytic, Thrombolytic Activities and Phytochemical Analysis of Simarouba glauca Leaves Extracts. Int. J. Pharm. Sci. Res. 5(2):432-437.


Molina A, Maria de los A, Brenes VG, Morales HD (1996). Description and viverization of 14 native forest species from tropical dry forest. Editorial FIELD SA.


Moron J, Merrien MA, Polonsky J (1971). Sur la biosynthe`se des quassinoı¨des de Simaruba glauca (Simarubaceae). J. Phytochem. 10:585.


Neergheen VS, Bahorun T, Pugo-Gunsam P, Ng Foong, Lin D, Ramful D, Aruoma OI (2010). Phenolic constituents and antioxidant efficacies of some mauritiana traditional preparations commonly used against cardiovascular disease. Int. J. Pharm. Phytochem. Res. 2(3):44-52.


Osagie-Eweka SDE, Orhue NEJ, Ekhaguosa OD (2016). Comparative phytochemical analyses and in-vitro antioxidant activity of aqueous and ethanol extracts of Simarouba glauca (Paradise Tree). Eur. J. Med. Plants 13(3):1-11.


Parul R, Kundu SK, Saha P (2013). In vitro nitric oxide scavenging activity of methanol extracts of three Bangladeshi medicinal plants. Pharm. Innov. J. 1(12):83-88.


Polonsky J (1978). The isolation and structure of 13, 18-dehydroglaucarubinone, a new antineoplastic quassinoid from Simarouba amara. Experientia 34(9):1122-1123.


Rang HP, Dale MM, Ritter JM, Moore PK (2003). Phytochemicals In: Pharmacology. 5th ed. Edinburgh: Churchill Livingstone. pp. 45-48.


Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans C (1999). Antioxidant activity applying an improved ABTS+ radical cation decolourization assay. Free. Rad. Biol. Med. 26:1231-1237.


Reddy PN, Ramachandraiah OS, Azeemoddin G, Jayaveera KN (2003). Processing of Simarouba glauca L. seed and oil. J. Oil Technol. Assoc. India 33(4):131-134.


Ruch RJ, Cheng SJ, Klaunig JE (1989). Prevention of cytotoxicity and inhibition of Intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10:1003-1008.


Sani I, Owoade A, Abdulhamid I, Fakai M, Bello F (2014). Evaluation of Physicochemical properties, Phytochemicals and Mineral Composition of Cocosnuciferal (Coconut) Kernel Oil. Int. J. Adv. Res. Chem. Sci. 1(8):22-30.


Santhi K, Sengottuvel R (2016). Qualitative and quantitative phytochemical analysis of Moringa Concanensis Nimmo. Int. J. Curr. Microbiol. Appl. Sci. 5(1):633-640.


Santhosh SK, Venugopal A, Radhakrishnan MC (2016). Study on the phytochemical, antibacterial and antioxidant activities of Simarouba glauca. South Indian J. Biol. Sci. 2(1):119‐124.


Shahriar M, Akhter S, Ismail H, Haque A, Bhuiyan MA (2012). Evaluation of in vitro antioxidant activity of bark extracts of Terminalia arjuna. J. Med. Plants Res. 6(39):5286-5298.


Sofowora AO (1993). Medicinal Plants and Traditional Medicine in Africa. University of Ife Press 2nd (ed). P 32.


Technical Data Report for Simarouba (Simarouba amara) (2002). Sage Press, Inc.


Trease GE, Evans WC (2002). Pharmacology. 15th Edn. Saunders. pp. 214-393.


Umesh TG (2014). In vitro callus induction and antioxidant potential of Decalepis hamiltonii (wight and arn). Int. J. Pharm. Sci. 6(6):452-456.


Umesh TG (2015). In-vitro antioxidant potential, free radical scavenging and cytotoxic activity of simarouba gluaca leaves. Int. J. Pharm. Pharm. Sci. 7(2):411-416.


Wenli Y, Yaping Z, Bo S (2004). The radical scavenging activities of radix puerariae isoflavonoids: A chemiluminescence study. Food Chem. 86:525-529.


Zhao H, Dong J, Lu J, Chen J, Li Y, Shan L (2006). Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeum vulgare L.). J. Agric. Food. Chem. 54(19):7277-7286.



APA Osagie-Eweka, S. D. E. (2018). Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree). African Journal of Plant Science, 12(1), 7-16.
Chicago Osagie-Eweka, S. D. E.. "Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)." African Journal of Plant Science 12, no. 1 (2018): 7-16.
MLA Osagie-Eweka, S. D. E.. "Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)." African Journal of Plant Science 12.1 (2018): 7-16.
DOI 10.5897/AJPS2017.1547

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