African Journal of Plant Science
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Article Number - C14A2BD66937


Vol.12(1), pp. 7-16 , January 2018
DOI: 10.5897/AJPS2017.1547
ISSN: 1996-0824



Full Length Research Paper

Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)



Osagie-Eweka, S. D. E.
  • Osagie-Eweka, S. D. E.
  • Department of Biochemistry, Faculty of Life Sciences, University of Benin, P. M. B. 1154, Benin City, Edo State, Nigeria.
  • Google Scholar







 Received: 15 March 2017  Accepted: 31 July 2017  Published: 31 January 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


This study was conducted to evaluate selected phytochemicals (antioxidant potentials of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca) relative to standard antioxidants. Sample was harvested, air dried, pulverized and extracted with aqueous and absolute methanol and ethanol; freeze dried at the National Energy Commission Centre, University of Benin. Alkaloids, phenols and tannins were identified, and also flavonoid was detected. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power activity, total antioxidant activity, hydroxyl radical activity, trolox equivalent antioxidant activity and nitric oxide (NO•) radical scavenging activity of stem bark extracts of S. glauca were evaluated, and all the experiment were conducted in a dose dependent manner. Butylated hydroxytuolene (BHT), ascorbate and trolox were introduced as positive controls (antioxidant). DPPH radical scavenging activity of stem bark extracts did not exhibit anti-radical activity at 50% inhibition but demonstrated less anti-radical activity at percentage inhibition lower than 50%. Extracts yielded significant reducing power and total antioxidant activities (FRAP). Hydroxyl radical scavenging activities of extracts was substantial; extracts exhibited significant anti-radical activity when trolox was introduced as positive antioxidant control, while nitric oxide scavenging activities was unprecedented. The presence of phytochemicals and antioxidant principles proffer high medicinal value to S. glauca.
 
Key words: Simarouba glauca, stem bark, oxidants, radical scavenging properties.

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APA Osagie-Eweka, S. D. E. (2018). Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree). African Journal of Plant Science, 12(1), 7-16.
Chicago Osagie-Eweka, S. D. E.. "Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)." African Journal of Plant Science 12, no. 1 (2018): 7-16.
MLA Osagie-Eweka, S. D. E.. "Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)." African Journal of Plant Science 12.1 (2018): 7-16.
   
DOI 10.5897/AJPS2017.1547
URL http://academicjournals.org/journal/AJPS/article-abstract/C14A2BD66937

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