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Article Number - 0B1584444931


Vol.9(1), pp. 1-11 , February 2014
DOI: 10.5897/BMBR2013.0216
ISSN: 1538-2273



Review

DNA microarrays and their applications in medical microbiology



Chijioke A. Nsofor
  • Chijioke A. Nsofor
  • Department of Biotechnology, Federal University of Technology, Owerri, Imo State, Nigeria.
  • Google Scholar







 Accepted: 02 November 2013  Published: 28 February 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Rapid diagnosis and treatment of disease is often based on the identification and characterization of causative agents derived from phenotypic characteristics. This can be laborious and time consuming, often requiring many skilled personnel and a large amount of lab space. However, the introduction of nucleic acid amplification techniques into molecular biology has transformed the laboratory detection of pathogens. The progression of the molecular diagnostic revolution currently relies on the ability to efficiently and accurately offer multiplex detection and characterization for a variety of infectious disease pathogens. DNA microarray analysis has the capability to offer robust multiplex detection. Multiple microarray platforms exist, including printed double-stranded DNA and oligonucleotide arrays, in situ-synthesized arrays, high-density bead arrays, electronic microarrays, and suspension bead arrays. The aim of this paper was to review DNA microarray technology, highlighting two major types: the oligonucleotide-based array and the PCR product-based array. Although, the use of microarrays to generate gene expression data has become routine, applications pertinent to microbiology continue to rapidly expand. This review highlights uses of microarray technology that impact diagnostic microbiology, including the detection and identification of pathogens, determination of antimicrobial resistance, epidemiological strain typing, and determination of virulence factors.

Key words: DNA microarray, applications, microbiology.

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APA Nsofor, C. A. (2014). DNA microarrays and their applications in medical microbiology. Biotechnology and Molecular Biology Reviews, 9(1), 1-11.
Chicago Chijioke A. Nsofor. "DNA microarrays and their applications in medical microbiology." Biotechnology and Molecular Biology Reviews 9, no. 1 (2014): 1-11.
MLA Chijioke A. Nsofor. "DNA microarrays and their applications in medical microbiology." Biotechnology and Molecular Biology Reviews 9.1 (2014): 1-11.
   
DOI 10.5897/BMBR2013.0216
URL http://academicjournals.org/journal/BMBR/article-abstract/0B1584444931

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