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Article Number - A2B926F63389


Vol.8(1), pp. 1-13 , March 2017
DOI: 10.5897/CRO16.0110
ISSN: 2141-2553



Full Length Research Paper

Methods for monitoring artemisinin-based combination therapies efficacy



Souleymane Dama
  • Souleymane Dama
  • Malaria Research and Training Center, Faculty of Pharmacy and Faculty of Medicine and Dentistry, University of Science, Techniques and Technologies of Bamako, P.O. Box1805, Mali
  • Google Scholar
Abdoulaye A. Djimde
  • Abdoulaye A. Djimde
  • Malaria Research and Training Center, Faculty of Pharmacy and Faculty of Medicine and Dentistry, University of Science, Techniques and Technologies of Bamako, P.O. Box1805, Mali
  • Google Scholar
Ogobara K. Doumbo
  • Ogobara K. Doumbo
  • Malaria Research and Training Center, Faculty of Pharmacy and Faculty of Medicine and Dentistry, University of Science, Techniques and Technologies of Bamako, P.O. Box1805, Mali
  • Google Scholar







 Received: 12 November 2016  Accepted: 17 January 2017  Published: 31 March 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Plasmodium falciparum resistance to artemisinin and its derivatives is spreading in South-East Asia, and there is growing concern that this may reach other endemic countries. Methods used to assess P.  falciparum resistance to artemisinin-based combination therapies (ACTs) are multiple and often divergent. This paper is a review of online accessible research publications from the past 20 years on ACTs, ranging from in vivo, in vitro/ex-vivo, molecular markers and pharmacokinetics studies. We highlight the procedures of the four main methods used for ACTs efficacy testing and provide a summary of published data. This review indicates that the most used method for ACT efficacy testing is the in vivo 28 days follow-up with molecular correction; the most widely used and reliable in vitro and ex-vivo method for artemisinin phenotyping is the ring stage survival assay from 0 to 3 h ring (RSA0-3h), and the main molecular marker of P. falciparum resistance to artemisinins are mutations on P. falciparum Kelch 13 propeller domain. Day 7 pharmacokinetics could help to predict resistance to artemether-lumefantrine and dihydroartemisinin-piperaquine. Findings from this review support that the combination of in vivo, in vitro/ex-vivo, molecular markers of drug resistance and day 7 PK levels of the partner drugs may be required for the optimal surveillance of artemisinin-based combination therapy efficacy in the field.

 

Key words: Malaria, artemisinin-based combination therapies (ACTs), monitoring, drug resistance, review.

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APA Dama, S., Djimde, A. A. & Doumbo, O. K. (2017). Methods for monitoring artemisinin-based combination therapies efficacy. Clinical Reviews and Opinions, 8(1), 1-13.
Chicago Souleymane Dama, Abdoulaye A. Djimde and Ogobara K. Doumbo. "Methods for monitoring artemisinin-based combination therapies efficacy." Clinical Reviews and Opinions 8, no. 1 (2017): 1-13.
MLA Souleymane Dama, Abdoulaye A. Djimde and Ogobara K. Doumbo. "Methods for monitoring artemisinin-based combination therapies efficacy." Clinical Reviews and Opinions 8.1 (2017): 1-13.
   
DOI 10.5897/CRO16.0110
URL http://academicjournals.org/journal/CRO/article-abstract/A2B926F63389

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