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Article Number - DD5C3364650


Vol.5(2), pp. 11-17 , February 2013
DOI: 10.5897/CRO11.022
ISSN: 2141-2553



Review

Recent trends in the diagnosis of toxoplasmosis



Vikrant Sudan
  • Vikrant Sudan
  • Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura- 281001, India.
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Amit K. Jaiswal
  • Amit K. Jaiswal
  • Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura- 281001, India.
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Daya Shanker
  • Daya Shanker
  • Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura- 281001, India.
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 Accepted: 11 January 2013  Published: 28 February 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Toxoplasma gondii is one of the most widely prevalent cyst forming apicomplexan parasite present worldwide. Felines serve as definitive hosts, while all non-feline vertebrates including humans, act as intermediate hosts of the parasite. There are several serological tests available for the detection of T. gondii antibodies such as the dye test (DT), indirect haemagglutination test (IHA), complement fixation test (CFT), modified agglutination test (MAT), latex agglutination test (LAT), indirect immunofluorescent test (IFAT) and direct agglutination test (DAT). The dye test once considered as gold standard is seldom used nowadays, owing to potent hazardous effects because of involvement of live tachyzoites. Of late, enzyme linked immune sorbent assays (ELISA) have gained a lot of utility in diagnosing toxoplasmosis. Other than serological tests, bioassays, polymerase chain reaction (PCR) based assays and histopathologies are also used. The various tests used for diagnosis of toxoplasmosis, along with various molecules being employed, coupled with their advantages and limitations, are described and discussed in detail in the present review.

 

Key words: Toxoplasma gondii, diagnosis, enzyme linked immune sorbent assays (ELISA), molecular techniques, polymerase chain reaction (PCR).


APA (2013). Recent trends in the diagnosis of toxoplasmosis. Clinical Reviews and Opinions, 5(2), 11-17.
Chicago Vikrant Sudan, Amit K. Jaiswal and Daya Shanker. "Recent trends in the diagnosis of toxoplasmosis." Clinical Reviews and Opinions 5, no. 2 (2013): 11-17.
MLA Vikrant Sudan, Amit K. Jaiswal and Daya Shanker. "Recent trends in the diagnosis of toxoplasmosis." Clinical Reviews and Opinions 5.2 (2013): 11-17.
   
DOI 10.5897/CRO11.022
URL http://academicjournals.org/journal/CRO/article-abstract/DD5C3364650

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