International Journal of Biotechnology and Molecular Biology Research
Subscribe to IJBMBR
Full Name*
Email Address*

Article Number - 0F0A98340897

Vol.4(6), pp. 86-92 , October 2013
ISSN: 2141-2154

 Total Views: 0
 Downloaded: 0

Full Length Research Paper

Micropropagation of the Indian Birthwort Arsitolochia indica L.

Syed Naseer Shah1, Amjad M. Husaini2* and Fatima Shirin1

1Genetics and Plant Propagation Division, Tropical Forest Research Institute, Mandla Road, Jabalpur 482 021, India.

2Centre for Plant Biotechnology, Division of Biotechnology, SKUAST-K, Shalimar, Srinagar-191121, India.

Email: [email protected]

 Accepted: 09 September 2013  Published: 31 October 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

Aristolochia indica L. is a medicinal woody perennial climber plant of immense pharmaceutical value. The species is endangered with possible extinction due to its indiscriminate harvesting as raw material for pharmaceutical industry, to manufacture drugs against cholera, inflammation, biliousness, dry cough and snake bite. A rigorous attempt has been made for development of in vitro propagation procedure for this species, involving four steps, namely: culture establishment, shoot multiplication, rooting and hardening. Aseptic cultures were established by growing nodal segments (1 to 1.5 cm) as explants on Murashige and Skoog (MS) medium containing 5.0 µM N6-Benzyladenine (BA).  Five nutrient media, MS, Woody Plant Medium (WPM), Gamborg Medium (B5), Nitsch and Nitsch Medium (NN), and Schenk and Hildebrandt Medium (SH) supplemented with different cytokinins and auxins at a concentration of 10.0 µM were used in this study. Ads at 10.0 µM proved optimum for in vitro shoot multiplication. The treatment resulted in 100% shoot number per explant at 15 days and 61.9% at 30 days on MS medium, 65.2% node number per shoot at 15 days and 196.2% at 30 days on WPM medium and 147.5 and 366.6% node number per explant at 30 days after inoculation on MS medium. The in vitro multiplied shoots were used for rooting experiment. Five nutrient media (MS, WPM, B5, NN and SH) and three auxin sources 10.0 µM each (IBA, IAA and NAA). SH medium with 10.0 µM NAA induced 327.8% rooting at 21days and 654.8% at 28 days and root number per explant 4300% at 21  and 394% at 28 day after inoculation. The in vitro propagated hardened plants exhibited excellent growth on transfer to natural condition.


Key words: Aristolochia indica L, in vitro propagation, N6-Benzyladenine.


Ansari SA, Sharma S, Pant NC, Mandal AK (2002). Synergism between IBA and thiamines for induction and growth of adventitious roots in Tectona grandis". J Sustain. For. 15:99-112.
Gamborg OL, Miller RA, Ojima K (1968). Nutrient requirements of suspension cultures of soya bean root cells, Exp. Cell Res. 50:151-58.
Gaspar T, Coumans M (1987). Root formation In: J.M. Bonga and D.J. Durzan (eds). Cell and Tissue Culture in Forestry, Specific Principles and Methods: Growth and Developments, Martinus Nihoff Publishers, Dordrecht, 2:202-217.
Jacobs WP (1972). The movement of plant hormones: auxins, gibberellins and cytokinins". In D.J. Carr (ed.), Plant Growth Substances, Springer, New York. pp. 701-709.
Kirtikar KR, Basu LM (1987). Indian Med. Plants. pp. 2117-2118.
Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot tip culture, Comb. Proc. Int. Plant prop. Soc. 30:421-27.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant. 15:473-97.
Nadagopal S, Ranjitha Kumari BD (2006). Adenine sulphate induced high frequency shoot organogenesis in callus and in vitro flowering of Cichoriumintybus L. cv. Focus - a potent medicinal plant, Acta Agric. Slov. 87(2):415-425.
Nitsch JP, Nitsch C (1969). Haploid plants from pollen grains, Sci. 163:85-87.
Pattar PV, Jayraj M (2012). In vitro Regeneration of Plantlets from Leaf and Nodal explants of Aristolochia indica L. - An important threatened medicinal plant. Asian Pac. J. Trop. Biomed. 2(2):488-493.
Rahman M (2001). Red data Book of Vascular plants. Bangladesh National Herbarium. Dhaka, Bangladesh.
Schenk RU, Hildebrandt AC (1972). Medium and technique for induction and growth of monocotyledonous and dicotyledonous plant cell culture, Can. J. Bot. 50:199-204.
Siddique NA, Bari MA, Pervin MM, Nahar N, Banu LA, Paul KK, Kabir MH, Huda AKMN, Ferdaus KMKB, Hossin MJ (2006a). Plant Regeneration from Axillary Shoots Derived Callus in Aristolochia indica Linn. an Endangered Medicinal Plant in Bangladesh, Pak. J Biol. Sci. 9:1320-323.
Siddique NA, Kabir MH, Bari MA (2006b). Comparative in vitro study of plant regeneration from nodal segments derived callus in Aristolochia indica Linn. and Hemidesmus indicus (L.) R. Br. endangered medicinal plants in Bangladesh, J. Plant Sci. 1(2):106-118.


APA (2013). Micropropagation of the Indian Birthwort Arsitolochia indica L.. International Journal of Biotechnology and Molecular Biology Research, 4(6), 86-92.
Chicago Syed Naseer Shah, Amjad M. Husaini and Fatima Shirin. "Micropropagation of the Indian Birthwort Arsitolochia indica L.." International Journal of Biotechnology and Molecular Biology Research 4, no. 6 (2013): 86-92.
MLA Syed Naseer Shah, Amjad M. Husaini and Fatima Shirin. "Micropropagation of the Indian Birthwort Arsitolochia indica L.." International Journal of Biotechnology and Molecular Biology Research 4.6 (2013): 86-92.

Subscription Form