International Journal of Biotechnology and Molecular Biology Research
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Article Number - 51E07B766771


Vol.8(4), pp. 38-48 , November 2017
https://doi.org/10.5897/IJBMBR2017.0272
ISSN: 2141-2154


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Full Length Research Paper

Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species



Teshome Edae
  • Teshome Edae
  • Department of Forestry, College of Agriculture and Natural Resources, Madda Walabu University P. O. Box 247, Bale Robe, Ethiopia.
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Melaku Alemu
  • Melaku Alemu
  • Ethiopian Institute of Agricultural Research (EIAR), National Agricultural Biotechnology Laboratory, Microbial Biotechnology Research. P. O. Box 31, Holetta, Ethiopia.
  • Google Scholar







 Received: 15 March 2017  Accepted: 12 October 2017  Published: 30 November 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The aim of the current study was to investigate the potential of Pleurotus species for laccase production on different lignocellulosic substrates and determine the optimal levels of physicochemical conditions required for the production. The study was conducted in National Agricultural Biotechnological Research Center, Holetta. The fruiting bodies of fungi were collected based on their morphology and inoculated on potato dextrose agar plate. Six different lignocellulosic substrates were collected and prepared for cultivation of Pleurotus species for laccase production. The highest enzyme production was obtained from bean straw compared to other substrates with an activity of 0.112 U/ml. The best three substrates; bean straw, Eucalyptus sawdust and wheat straw were selected, and a mixture of each of them on equal proportions was tested for laccase production potential. A mixture of Eucalyptus sawdust and bean straw on equal proportion was found to be the best, showing an activity of 0.137 U/ml and hence selected for different parameter optimization. Optimal laccase production (0.292 U/ml) was obtained on the 10th day of incubation period and the optimum temperature and pH were 28°C and 5.5, respectively. Soluble starch and peptone were found to be the most preferred carbon and nitrogen sources for laccase production, respectively. Asparagine and alanine induced more laccase production, with asparagine being the most potent inducer.
 
Key words: Pleurotus species, lignocellulosic substrates, bean straw, Eucalyptus sawdust, laccase.

Abdel-Azeem AM, Salem FM (2012). Biodiversity of laccase producing fungi in Egypt. Mycosphere 36:900-920.

 

Abdulah AR (2008). The use of partially purified laccase produced by some fungal isolates in Aflatoxin B1 degradation. M.Sc. Thesis in Biotechnology, University of Baghdad College of Science Department of Biotechnology.

 
 

Adejoye OD, Fasidi IO (2009). Effect of cultural conditions on biomass and laccase production in submerged medium by Schizophylllum commune (FR.). A Nigerian edible mushrooms. Electronic J. Environ. Agric. Food Chem.8(11):1186-1193.

 
 

Arora DS, Gill PK (2001). Effects of various media and supplements on laccase production by some white rot fungi. Biol. Resour. Technol. 77:89-91.
Crossref

 
 

Arora DS, Sharma RK (2010). Ligninolytic fungal laccase and their biotechnological applications. Appl. Biochem. Biotechnol. 160:1760-1788.
Crossref

 
 

Bakkiyaraj S, Aravindan R, Arrivukkarasan S, Viruthagiri T (2013). Enhanced laccase production by Trametes hirusta using wheat bran under submerged fermentation. Int. J. Chem. Technol. Res. 5(3):1224-1238.

 
 

Baldrian P (2004). Purification and characterization of laccase from the white rot fungus Daedalea quercina and decolorization of synthetic dyes by the enzyme. Appl. Microbiol. Biotechnol. 63:560-563.
Crossref

 
 

Bollag JM, Leonowicz A (1984). Comparative studies of extracellular fungal laccase. Appl. Environ. Microbiol. 48:849-854.

 
 

Demir A, Aytar P, Gedikli S, Çabuk A, Arısoy M (2011). Laccase production with submerged and solid-state fermentation: Benefit and cost analysis. J. Biol. Chem. 39(3):305-313.

 
 

Elsayed MA, Hassan MM, Elshafei AM, Haroun BM, Othman AM (2012). Optimization of cultural and nutritional parameters for the production of laccase by Pleurotus ostreatus ARC280. Br. Biotechnol. J. 2:115-132.
Crossref

 
 

Hashim AJ (2012). Determination of optimal conditions for laccase production by Pleurotus ostreatus using sawdust as solid medium and its use in phenol degradation. Baghdad Sci. J. 9(3):491-499.

 
 

Irshad M, Asgher M (2011). Production and optimization of ligninolytic enzymes by white rot fungus Schizophyllum commune IBL-06 in solid state medium banana stalks. Afr. J. Biotechnol. 10(79):18234-18242.

 
 

Johnsy G, Kaviyarasan V (2014). Effect of physico-chemical parameters on ligninolytic enzyme production of an indigenous isolate of Neolentinus kauffmanii -under submerged culture condition. World J. Pharmaceut. Sci. 2(5):464-468.

 
 

Junyao H, Ye X, Ling Q, Dong L (2014). Enhanced production of an acid-tolerant laccase by cultivation of Armillariel latabescens. J. Chem. Pharmaceut. Res. 6(1):240-245.

 
 

Kalra K, Chauhan R, Shavez M, Sachdeva S (2013). Isolation of laccase producing Trichoderma Spp. and effect of pH and temperature on its activity. Int. J. Chem. Technol. Res. 5(5):2229-2235.

 
 

Kenkebashvili N, Elisashvili V, Wasser SP (2012). Effect of carbon, nitrogen sources, and copper concentration on the ligninolytic enzyme production by Coriolopsis gallica. J. Waste Convers. Bioproducts Biotechnol. 1(2):22-27.

 
 

Kiiskinen LL, Palonen H, Linder M, Viikari L, Kruus K (2004). Laccase from Melanocarpus albomyces binds effectively to cellulose. FEBS Lett. 576:251-255.
Crossref

 
 

Kim Y, Nicell JA (2006). Impact of reaction conditions on the laccase catalyzed conversion of bisphenol A. Bioresour. Technol. 97:1431-1442.
Crossref

 
 

Kumar VV, Kirupha SD, Periyaraman P, Sivanesan S (2011). Screening and induction of laccase activity in fungal species and its application in dye decolorization. Afr. J. Microbiol. Res. 5:1261-1267.
Crossref

 
 

Kumari J, Negi S (2014). Development of bioprocess for the production of laccase by Pleurotus ostreatus MTCC 1802 using evolutionary optimization technique. Indian J. Exp. Biol. 52:1106-1111.

 
 

Mongkolthanaruk W, Tongbopit S, Bhoonobtong A (2012). Independent behavior of bacterial laccases to inducers and metal ions during production and activity. Afr. J. Biotechnol. 11(39):9391-9398.
Crossref

 
 

Nadeem A, Baig S, Sheikh N (2014). Mycotechnological production of laccase by Pleurotus ostreatus P1 and its inhibition study. J. Anim. Plant Sci. 24(2):492-502.

 
 

Naraian R, Singh D, Verma A, Garg SK (2010). Studies on in vitro degradability of mixed crude enzyme extracts produced from Pleurotus spp. J. Environ. Biol. 31(6):945-951.

 
 

Organisation for Economic Co-operation and Development (OECD) (2005). Consensus Document on the Biology of Pleurotus spp. (Oyster Mushroom). Environment, Health and Safety Publications Series on Harmonisation of Regulatory Oversight in Biotechnology. André-Pascal Press, Paris France 34:10-15.

 
 

Patel H, Gupte A, Gupte S (2009). Effect of different culture condition and inducer on production of laccae by basidiomycetes fungal isolate Pleurotus ostreatus HP-1 under solid state fermentation. Bio- resource 4(1):268-284.

 
 

Patrick F, Mtui G, Mshandete AM, Johansson G, Kivaisi A (2009). Purification and characterization of a laccase from the basidiomycete Funaliatrogii (Berk.) isolated in Tanzania. Afr. J. Biochem. Res. 3(5):250-258.

 
 

Patrick F, Mtui G, Mshandete AM, Kivaisi A (2011). Optimization of laccase and manganese peroxidase production in submerged culture of Pleurotus sajor-caju. Afr. J. Biotechnol. 10(50):10166-10177.

 
 

Ping W, Xuerong F, Li C, Qiang W, Aihui Z (2008). Decolorization of reactive dyes by laccase immobilized in alginate/gelatin blent with PEG. J. Environ. Sci. 20:1519-1522.
Crossref

 
 

Prasad KK, Venkata Mohan S, Vijaya Bhaskar Y, Ramanaiah SV, Lalit Babu V, Pati BR, Sarma PN (2005). Laccase Production using Pleurotus ostreatus 1804 immobilized on PUF cubes in batch and packed bed reactors: Influence of culture conditions. J. Microbiol. 43(3):301-307.

 
 

Selim MS, Mahmoud MG, Rifaat HM, El Sayed OH, El Beih FM (2013). Effect of inducers and process parameters on laccase production by locally isolated marine Streptomyces lydicus from Red Sea. Egypt. Int. J. Chem. Technol. Res. 5(1):15-23.

 
 

Sivakami V, Ramachandran B, Srivathsan J, Kesavaperumal G, Smily B, Kumar JM (2012). Production and optimization of laccase and lignin peroxidase by newly isolated Pleurotus ostreatus LIG 19. J. Microbiol. Biotechnol. Res. 2(6):875-881.

 
 

Sivakumar R, Rajendran R, Balakumar C, Tamilvendan M (2010). Isolation, screening and optimization of production medium for thermostable laccase production from Ganoderma sp. Int. J. Eng. Sci. Technol. 2(12):7133-7141.

 
 

Snajdr J, Baldrain P (2007). Temperature effect the production, activity and stability of ligninolytic enzymes in Pleurotus ostreatus and Trametes versicolor. Folia Microbial. 52(5):498-502.
Crossref

 
 

Ticlo DD, Verma AK, Mathew M, Raghukumar C (2006). Effect of nutrient nitrogen on laccase production, its isozyme pattern and effluent decolorization by the fungus NIOCC #2a, isolated from mangrove wood. Indian J. Mar. Sci. 35(4):364-372.

 
 

Viswanath B, Rajesh B, Janardhan A, Kumar AP, Narasimha G (2014). fungal laccase and their applications in bioremediation. Enzyme Res. 1-21.
Crossref

 

 


APA Edae, T., & Alemu, M. (2017). Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species. International Journal of Biotechnology and Molecular Biology Research, 8(4), 38-48.
Chicago Teshome Edae and Melaku Alemu. "Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species." International Journal of Biotechnology and Molecular Biology Research 8, no. 4 (2017): 38-48.
MLA Teshome Edae and Melaku Alemu. "Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species." International Journal of Biotechnology and Molecular Biology Research 8.4 (2017): 38-48.
   
DOI https://doi.org/10.5897/IJBMBR2017.0272
URL http://academicjournals.org/journal/IJBMBR/article-abstract/51E07B766771

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