International Journal of Biotechnology and Molecular Biology Research
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Article Number - 51E07B766771

Vol.8(4), pp. 38-48 , November 2017
ISSN: 2141-2154

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Full Length Research Paper

Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species

Teshome Edae
  • Teshome Edae
  • Department of Forestry, College of Agriculture and Natural Resources, Madda Walabu University P. O. Box 247, Bale Robe, Ethiopia.
  • Google Scholar
Melaku Alemu
  • Melaku Alemu
  • Ethiopian Institute of Agricultural Research (EIAR), National Agricultural Biotechnology Laboratory, Microbial Biotechnology Research. P. O. Box 31, Holetta, Ethiopia.
  • Google Scholar

 Received: 15 March 2017  Accepted: 12 October 2017  Published: 30 November 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

The aim of the current study was to investigate the potential of Pleurotus species for laccase production on different lignocellulosic substrates and determine the optimal levels of physicochemical conditions required for the production. The study was conducted in National Agricultural Biotechnological Research Center, Holetta. The fruiting bodies of fungi were collected based on their morphology and inoculated on potato dextrose agar plate. Six different lignocellulosic substrates were collected and prepared for cultivation of Pleurotus species for laccase production. The highest enzyme production was obtained from bean straw compared to other substrates with an activity of 0.112 U/ml. The best three substrates; bean straw, Eucalyptus sawdust and wheat straw were selected, and a mixture of each of them on equal proportions was tested for laccase production potential. A mixture of Eucalyptus sawdust and bean straw on equal proportion was found to be the best, showing an activity of 0.137 U/ml and hence selected for different parameter optimization. Optimal laccase production (0.292 U/ml) was obtained on the 10th day of incubation period and the optimum temperature and pH were 28°C and 5.5, respectively. Soluble starch and peptone were found to be the most preferred carbon and nitrogen sources for laccase production, respectively. Asparagine and alanine induced more laccase production, with asparagine being the most potent inducer.
Key words: Pleurotus species, lignocellulosic substrates, bean straw, Eucalyptus sawdust, laccase.

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APA Edae, T., & Alemu, M. (2017). Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species. International Journal of Biotechnology and Molecular Biology Research, 8(4), 38-48.
Chicago Teshome Edae and Melaku Alemu. "Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species." International Journal of Biotechnology and Molecular Biology Research 8, no. 4 (2017): 38-48.
MLA Teshome Edae and Melaku Alemu. "Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species." International Journal of Biotechnology and Molecular Biology Research 8.4 (2017): 38-48.

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