International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 97

Full Length Research Paper

Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars

Tomader Errabii
  • Tomader Errabii
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar
Christophe Bernard Gandonou
  • Christophe Bernard Gandonou
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar
Samira Bouhdid
  • Samira Bouhdid
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar
Jamal Abrini
  • Jamal Abrini
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar
Nadia Skali-Senhaji
  • Nadia Skali-Senhaji
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar


  •  Received: 01 April 2017
  •  Accepted: 07 June 2017
  •  Published: 30 June 2017

Abstract

In this work, the effect of different concentrations of NaCl on calli induced from two sugarcane cultivars NCo310 and CP59-73 was studied. Growth and ion concentrations (Na+, Cl-, K+ and Ca2+) of calli were determined after 1, 2 and 3 months of stress with the objective to understand the cellular mechanisms operating in salt stress tolerance and to determine the implication of inorganic fraction in salt tolerance in sugarcane cultivars. A negative effect of the NaCl concentration and the duration of stress exposure on the callus rate growth was observed in both cultivars and with more extent in CP59-73 cv. Results showed an increase in Na+ and Cl and a decrease in K+ and Ca2+ concentrations after 1, 2 and 3 months of salt stress exposure. It also showed that resistant cv. NCo310 stressed calli accumulated less Na+ and retained more K+ and Ca2+ than CP59-73 calli. Cl- appeared to be involved in osmotic adjustment since the resistant cv. NCo310 stressed calli accumulated more Cl- than CP59-73 ones. These results suggested that the resistance to salinity in sugarcane is associated with a high K+, Ca2+ and Cl- concentrations and a low Na+ concentration within cells.

Key words:  sugarcane (Saccharum sp.), salt stress, ion uptake, callus growth, long-term stress exposure.

Abbreviation

RGR, Relative growth rate; cv, cultivar; MS medium, Murashige and Skoog medium; FW, fresh weight; DW, dry weight.