International Journal of Biotechnology and Molecular Biology Research
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Article Number - 57B0B2264888


Vol.8(1), pp. 1-9 , June 2017
https://doi.org/10.5897/IJBMBR2017.0273
ISSN: 2141-2154


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Full Length Research Paper

Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars



Tomader Errabii
  • Tomader Errabii
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar
Christophe Bernard Gandonou
  • Christophe Bernard Gandonou
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar
Samira Bouhdid
  • Samira Bouhdid
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar
Jamal Abrini
  • Jamal Abrini
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar
Nadia Skali-Senhaji
  • Nadia Skali-Senhaji
  • Laboratoire de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
  • Google Scholar







 Received: 01 April 2017  Accepted: 07 June 2017  Published: 30 June 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In this work, the effect of different concentrations of NaCl on calli induced from two sugarcane cultivars NCo310 and CP59-73 was studied. Growth and ion concentrations (Na+, Cl-, K+ and Ca2+) of calli were determined after 1, 2 and 3 months of stress with the objective to understand the cellular mechanisms operating in salt stress tolerance and to determine the implication of inorganic fraction in salt tolerance in sugarcane cultivars. A negative effect of the NaCl concentration and the duration of stress exposure on the callus rate growth was observed in both cultivars and with more extent in CP59-73 cv. Results showed an increase in Na+ and Cl and a decrease in K+ and Ca2+ concentrations after 1, 2 and 3 months of salt stress exposure. It also showed that resistant cv. NCo310 stressed calli accumulated less Na+ and retained more K+ and Ca2+ than CP59-73 calli. Cl- appeared to be involved in osmotic adjustment since the resistant cv. NCo310 stressed calli accumulated more Cl- than CP59-73 ones. These results suggested that the resistance to salinity in sugarcane is associated with a high K+, Ca2+ and Cl- concentrations and a low Na+ concentration within cells.

Key words:  sugarcane (Saccharum sp.), salt stress, ion uptake, callus growth, long-term stress exposure.

Abbreviation:

RGR, Relative growth rate; cv, cultivar; MS medium, Murashige and Skoog medium; FW, fresh weight; DW, dry weight.


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APA Errabii, T., Gandonou, C. B., Bouhdid, S., Abrini, J., & Skali-Senhaji, N. (2017). Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars. International Journal of Biotechnology and Molecular Biology Research, 8(1), 1-9.
Chicago Tomader Errabii, Christophe Bernard Gandonou, Samira Bouhdid, Jamal Abrini and Nadia Skali-Senhaji. "Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars." International Journal of Biotechnology and Molecular Biology Research 8, no. 1 (2017): 1-9.
MLA Tomader Errabii, et al. "Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars." International Journal of Biotechnology and Molecular Biology Research 8.1 (2017): 1-9.
   
DOI https://doi.org/10.5897/IJBMBR2017.0273
URL http://academicjournals.org/journal/IJBMBR/article-abstract/57B0B2264888

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