International Journal of Biotechnology and Molecular Biology Research
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Article Number - 5D4D34241264


Vol.1(4), pp. 42-46 , July 2010

ISSN: 2141-2154



Full Length Research Paper

Antimutagenic activity of aqueous extract of Momordica charantia


Meera Sumanth* and G. Nagarjuna Chowdary




 

Department of Pharmacology, Visveswarapura Institute of Pharmaceutical Sciences, 22nd Main, 24th Cross, B. S. K II stage, Bangalore-560070, Karnataka, India.


Email: meerasumanth@gmail.com






 Accepted: 18 May 2010  Published: 31 July 2010

Copyright © 2010 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

Toxicological studies have undergone a significant evolution during the past decade, with inclusion and great emphasis on chronic toxicity, carcinogenicity, teratogenicity and mutagenicity. Present study was taken up to evaluate antimutagenicity of aqueous extract of Momordica charantia (MC) by bone marrow micronucleus assay (MNT) and chromosomal aberration test (CAT) in mice. Cyclophosphamide (100 mg/kg, i.p) was used as a genotoxic challenge and bone marrow of control and MC treated mice was collected after 24, 48 and 72 h, respectively. In MNT, the bone marrow smears were stained with May-Grunwald’s followed by Giemsa stain. Polychromatic and Normochromatic erythrocytes were counted and P/N ratio was calculated. In CAT, colchicine, four mg/kg, i.p, was administered 90 min before sacrifice, bone marrow smears were prepared, stained with Giemsa stain and observed under 100X for different types of chromosomal aberrations. Mitotic index was calculated. The MC has significantly decreased the formation of micronuclei, increased the P/N ratio, inhibited the formation of chromosomal aberrations and increased the mitotic index. Hence, M. charantia has significant antimutagenic activity.

Key words: Antimutagenic activity, chromosomal aberration test, micronucleus assay, Momordica charantia.

 


APA (2010). Antimutagenic activity of aqueous extract of Momordica charantia. International Journal of Biotechnology and Molecular Biology Research, 1(4), 42-46.
Chicago Meera Sumanth and G. Nagarjuna Chowdary. "Antimutagenic activity of aqueous extract of Momordica charantia." International Journal of Biotechnology and Molecular Biology Research 1, no. 4 (2010): 42-46.
MLA Meera Sumanth and G. Nagarjuna Chowdary. "Antimutagenic activity of aqueous extract of Momordica charantia." International Journal of Biotechnology and Molecular Biology Research 1.4 (2010): 42-46.
   
DOI
URL http://academicjournals.org/journal/IJBMBR/article-abstract/5D4D34241264

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