Full Length Research Paper
Mariana Carmen Chifiriuc1*, Coralia Bleotu2, Diana-Roxana Pelinescu1, Veronica Lazar1, Lia-Mara Ditu1, Tatiana Vassu1, Ileana Stoica1, Olguta Dracea3, Ionela Avram1 and Elena Sasarman1
1Department of Microbiology, MICROGEN (Center for Research in Genetics, Microbiology and Biotechnology), Faculty of Biology, University of Bucharest, Ale. Portocalilor 1-3, Sector 5, 77206-Bucharest, Romania.
The purpose of this study was to investigate by in vitro studies the antimicrobial activity of eight lactic acid bacterial (LAB) strains belonging to Lactobacillus paracasei spp. paracasei, L. plantarum and L. rhamnosus species against Salmonella enteritidis, Shigella flexneri and EPEC pathogenic strains isolated from pediatric diarrhoea cases, simultaneously with the assessment of the cytotoxicity and immunomodulatory potential of the respective strains. The study of the adherence capacity to the cellular substrate represented by HeLa cells was performed by Cravioto’s adapted method. The cytotoxicity was determined on HeLa cells and the level of soluble pro- and anti-inflammatory cytokines was assessed by ELISA. Our in vitro studies are demonstrating that the selected probiotic strains are inhibiting the adherence and colonization of HeLa cells by the enteropathogenic strains isolated from pediatric diarrhoea mainly by direct competition for adherence sites, demonstrating their potential use in the treatment of pediatric gastro-intestinal disorders, as an alternative to/in association with antibiotics. A great advantage of the selected probiotic strains is their low cytotoxicity and ability to trigger a beneficial cytokine response in the epithelial cells, which potentiates their antimicrobial activity by stimulating the occurrence of a rapid immune response following the intestinal injury.
Key words: Antimicrobial activity, immunomodulatory, cytokines, invasive bacteria, Lactobacillus, enteropathogenic bacteria, antimicrobial effect, cytotoxicity, probiotics.
|APA||(2010). Patterns of colonization and immune response elicited from interactions between enteropathogenic bacteria, epithelial cells and probiotic fractions. International Journal of Biotechnology and Molecular Biology Research, 1(4), 47-57.|
|Chicago||Mariana Carmen Chifiriuc, Coralia Bleotu, Diana-Roxana Pelinescu, Veronica Lazar, Lia-Mara Ditu, Tatiana Vassu, Ileana Stoica, Olguta Dracea, Ionela Avram and Elena Sasarman. "Patterns of colonization and immune response elicited from interactions between enteropathogenic bacteria, epithelial cells and probiotic fractions." International Journal of Biotechnology and Molecular Biology Research 1, no. 4 (2010): 47-57.|
|MLA||Mariana Carmen Chifiriuc, et al. "Patterns of colonization and immune response elicited from interactions between enteropathogenic bacteria, epithelial cells and probiotic fractions." International Journal of Biotechnology and Molecular Biology Research 1.4 (2010): 47-57.|