International Journal of Biotechnology and Molecular Biology Research
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Article Number - 80B1F3A2211


Vol.3(2), pp. 15-21 , March 2012
DOI: 10.5897/IJBMBR11.052
ISSN: 2141-2154



Full Length Research Paper

Chromium stress on peroxidase, ascorbate peroxidase and acid invertase in pea (Pisum sativum L.) seedling


Surekha and Joginder Singh Duhan2*




1Department of Botany, Government National Post-Graduate College, Sirsa-125005, India.

2Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-125055, India.


Email: duhanjs68@gmail.com, duhanjs@rediffmail.com






 Accepted: 20 February 2012  Published: 31 March 2012

Copyright © 2012 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In this investigation, chromium (Cr) toxicity on enzymes like peroxidase, ascorbate peroxidase and acid invertase was studied in pea seedling to evaluate the relative tolerance of the different pea varieties. Effect of Cr (0, 1.0, 2.0, 3.0 mM) on different parts of the seedling that is, cotyledon, plumule and radicle were studied separately to have a comparative study of the enzyme activity in these parts of the plants and its varieties. Acid invertase activity in cotyledons of Arkel was not affected significantly by chromium but increased in Rachna and HFP-8712. Chromium decreased acid invertase activity in radicle and plumule. Chromium treatment increased peroxidase activity in all component of seedling and found to be highest in untreated cotyledons of Arkel. Ascorbate peroxidase activity in the radicle and plumule was higher in Rachna. Enzyme activity decreased significantly with the increasing chromium treatment in cotyledons of Arkel while converse was true with regard to HFP-8712. These results indicate that Rachna is more tolerant followed by HFP-8712 and Arkel.

Key words: Chromium toxicity, peroxidase, ascorbate peroxidise, acid invertase, Pisum sativum.

Abbreviation:

Abbreviations: ROS, Reactive oxygen species; MTS, metallothioneins; POX, peroxidase; CAT, catalase; GPX,guaiacol peroxidise; GR, glutathione reductase; APX, ascorbate peroxidise; SOD, superoxide dismutase; Mn-SOD, Mn-superoxide dismutase.


 

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APA (2012). Chromium stress on peroxidase, ascorbate peroxidase and acid invertase in pea (Pisum sativum L.) seedling. International Journal of Biotechnology and Molecular Biology Research, 3(2), 15-21.
Chicago Surekha and Joginder Singh Duhan. "Chromium stress on peroxidase, ascorbate peroxidase and acid invertase in pea (Pisum sativum L.) seedling." International Journal of Biotechnology and Molecular Biology Research 3, no. 2 (2012): 15-21.
MLA Surekha and Joginder Singh Duhan. "Chromium stress on peroxidase, ascorbate peroxidase and acid invertase in pea (Pisum sativum L.) seedling." International Journal of Biotechnology and Molecular Biology Research 3.2 (2012): 15-21.
   
DOI 10.5897/IJBMBR11.052
URL http://academicjournals.org/journal/IJBMBR/article-abstract/80B1F3A2211

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