International Journal of Biotechnology and Molecular Biology Research
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Article Number - 90909E059065


Vol.7(2), pp. 20-28 , June 2016
DOI: 10.5897/IJBMBR2015.0245
ISSN: 2141-2154



Full Length Research Paper

Shoot nodal culture and virus indexing of selected local and improved genotypes of cassava (Manihot esculenta) from Sierra Leone



Janatu V. Sesay
  • Janatu V. Sesay
  • Germplasm Enhancement and Seeds Systems, Njala Agricultural Research Centre (NARC), P. M. B 540, Freetown, Sierra Leone.
  • Google Scholar
Kwadwo O. Ayeh
  • Kwadwo O. Ayeh
  • Department of Botany, University of Ghana, Box LG 55, Legon, Accra, Ghana.
  • Google Scholar
Prince E. Norman*
  • Prince E. Norman*
  • Germplasm Enhancement and Seeds Systems, Njala Agricultural Research Centre (NARC), P. M. B 540, Freetown, Sierra Leone.
  • Google Scholar
E. Acheampong
  • E. Acheampong
  • Department of Botany, University of Ghana, Box LG 55, Legon, Accra, Ghana.
  • Google Scholar







 Received: 21 December 2015  Accepted: 06 June 2016  Published: 30 June 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Cassava (Manihotes culenta Crantz) is among major food and income security crops in sub-Saharan Africa. However, high seed dormancy and delayed germination limit seed propagation. Using traditional stem cutting causes loss of superior genotypes and decreases productivity as a result of low multiplication ratio (1:10) and viral and bacterial diseases.Thus, the aim of this study was to optimize and screen efficient in vitro protocols for rapid multiplication and production of disease-free cassava planting materials through nodal culture technique. The experiment was laid out in a 5 x 5 factorial arrangement in Completely Randomized Design with five cassava genotypes (Warima, Munafa, SLICASS 6, Coco cassada and SLICASS 7) and  five BAP supplemented MS medium (0, 0.02, 0.1, 1.0 and 2.0 mg/L), replicated three times. Results revealed that, BAP supplement significantly (p<0.001) influenced numbers of leaves and shoots compared to plantlet height growth traits of nodal cassava explants in culture. Virus indexing of infected plants from screen house using species specific primer pairs, OjaRep/EACVMRep and OjaRep/ACMVRep, confirmed the presence of East Africa Cassava Mosaic Virus (EACMV) and the African Cassava Mosaic Virus (ACMV) at an amplicon of approximately 650bp and 400bp, respectively. The study demonstrated the effectiveness of cytokinin supplemented MS medium in enhancing growth of cassava; and the adequacy and effectiveness of the PCR technique in identifying ACMV and EACMV using nodal cuttings. Future studies will involve molecular characterization of the EACMV strain(s) existing in the country.

Key words: Cassava, nodal culture, virus indexing.

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APA Sesay, J. V., Ayeh, K. O., Norman, P. E., & Acheampong, E. (2016). Shoot nodal culture and virus indexing of selected local and improved genotypes of cassava (Manihot esculenta) from Sierra Leone. International Journal of Biotechnology and Molecular Biology Research, 7(2), 20-28.
Chicago Janatu V. Sesay, Kwadwo O. Ayeh, Prince E. Norman and E. Acheampong. "Shoot nodal culture and virus indexing of selected local and improved genotypes of cassava (Manihot esculenta) from Sierra Leone." International Journal of Biotechnology and Molecular Biology Research 7, no. 2 (2016): 20-28.
MLA Janatu V. Sesay, et al. "Shoot nodal culture and virus indexing of selected local and improved genotypes of cassava (Manihot esculenta) from Sierra Leone." International Journal of Biotechnology and Molecular Biology Research 7.2 (2016): 20-28.
   
DOI 10.5897/IJBMBR2015.0245
URL http://academicjournals.org/journal/IJBMBR/article-abstract/90909E059065

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