International Journal of Biotechnology and Molecular Biology Research
Subscribe to IJBMBR
Full Name*
Email Address*

Article Number - A95DCF440379

Vol.4(5), pp. 78-85 , September 2013
ISSN: 2141-2154

 Total Views: 0
 Downloaded: 0

Full Length Research Paper

Micropropagation of Litsea glutinosa (Lour) C.B

Syed Naseer Shah1, Amjad M. Husaini2* and S. A. Ansari1



1Genetics and Plant Propagation Division, Tropical Forest Research Institute, Mandla Road, Jabalpur 482 021, India.
2Centre for Plant Biotechnology, Division of Biotechnology, SKUAST-K, Shalimar, Srinagar-191121, J&K, India.

Email: [email protected]

 Accepted: 09 September 2013  Published: 30 September 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0



Litsea glutinosa (Lour) C.B (Hindi: Maida lakri) is a medicinal plant of immense pharmaceutical value. The species is critically endangered due to its indiscriminate collection as raw material for pharmaceutical industry, where it is used for manufacturing drugs for pain, arousing sexual power and in treatment of diarrhea and dysentery etc. An attempt has been made for development of in vitro propagation procedure for the species, involving four steps, namely: culture establishment, shoot multiplication, rooting and hardening. Aseptic cultures were established on Murashige and Skoog (MS) medium supplemented 10.0 µM N6-benzyladenine (BA) using nodal segments (1 cm). Four sets of simple randomized experiment were carried out on MS medium to study the effect of four doses of each BA, GA3, IAA, (0, 2.5, 5.0 and 10 µM) and ascorbic acid (0, 284, 852 and 1136 µM) for in vitro shoot multiplication. MS medium supplemented with 5.0 µM BA with 852 µM ascorbic acid significantly proved optimum for in vitro shoot multiplication and resulted in 1.05 shoot number explant-1, 1.72 node number shoot-1 and 1.79 node number explant-1 at one month after inoculation. The in vitro multiplied shoots were tested for in vitro root induction on MS culture media containing auxin IBA (Indole-3-butaric acid) treatments (0, 2.5, 5.0 and10.0 µM) in simple randomized designs experiment. MS media supplemented with 10.0 µM IBA, screened out to be significantly excellent for induction and growth of adventitious roots, resulting in 72.2% rooting and 0.72 root number explant-1 at 30 days after inoculation. The in vitro propagated plants exhibited excellent growth. Therefore, the present study recommends a four step micropropagation procedure for in vitro production of L. glutinosa plants on a commercial scale to meet the requirement of pharmaceutical industries and save the species from extinction.
Key words: Litsea glutinosa (Lour) C.B, ascorbic acid, nodal segments.


Ahuja MR (1991). Biotechnology in forest trees. Plant Res. Dev. 33:106-120.
Ali A, Rizwan M, Majid A, Saleem A, Naveed NH (2012). Effect of media type and explants source on micropropagation of Dalbergia sissoo: A tree of medicinal importance. J. Med. Plants Res. 9:1742-1751.
Bathoju G, Giri A (2012). Production of medicinally important secondary metabolites (stigmasterol andhecogenin) from root cultures of Chlorophytum borivilianum (Safed musli), Recent Res. Sci. Technol. 5:45-48.
Berlyn GP, Beck RC (1980). Tissue culture as a technique for studying meristematic activity. In Control of Shoot Growth in Trees. Proceedings of the Joint Workshop of IUFRO Working Parties in Xylem and Shoot Growth Physiology. pp.305-324.
Casson SA, Lindsey K (2003). Genes and signaling in root development. New Phytol. 158:11-38.
Gianfagna T (1995). Natural and synthetic growth regulators and their use in horticultural and agronomic crops. In Plant Hormones: Physiology, Biochemistry and Molecular Biology, Davies, P.J. (ed.) Kluwer Academic Publishers, Dordrecht. pp.751-773.
Hosamath PV (2011). Evaluation of Antimicrobial activity of Litsea glutinosa, Int. J. Pharm. Appl. 1:105-114.
Jarvis BC, Booth A (1981). Influence of IBA, boron, myoinositol, vitamin D and seedling age on adventitious root development in cuttings of Phaseolus aureus. Physiology Plant. 53:213- 218.
Karthtikeyan K, Chandran C, Kulothungan S (2008) Rapid clonal multi-plication through in vitro axillary shoot proliferation of Centella asiatica L. Indian Journal of Biotechnology. 8:232-235.
Kolar AB, Vivekanandan L, Ghouse BM (2008). In vitro regeneration and flower induction on Solanum nigrum L. from pachamalai hills of eastern ghats, Plant Tissue Cult. Biotech. 1:43-48.
Kritikar K, Basu BD (1981). Indian Medicinal Plant, Periodic Book Agency, Delhi, India, III. pp. 2158-2160.
Petrova M, Zayova E, Vitkova A (2011). Effect of silver nitrate on in vitro root formation of Gentiana lutea AC Romanian Biotechnol. Lett. 16:53-58.
Rabena AR (2010). Propagation Techniques of Endangered Sablot (Litsea gultinosa) Lour. C.B. Rob. Nat. Peer Rev. J. 5:56-77.
Reddy KN, Reddy CS (2008). First red list of medicinal plants of Andhra Pradesh, India- Conservation Assessment and Management Planing. Ethnobot. Leafl.12:103-107.
Richard WJ, Patel KR, Thorpe TA (1988). Ascorbic acid enhancement of organogenesis in tobacco callus, Plant Cell, Tiss. Organ Cult. 13:219-228.
Rumary C, Thorpe TA (1984). Plantlet formation in black and white spruce. Canadian J. For. Res. 14:10-16.
Sharma N, Chandel KPS (1992). Effects of ascorbic acid on axillary shoot induction in Tylophora indica (Burm. F.) Merrill. Plant Cell, Tiss. Organ Cult. 29:109-113.
Sharma S, Kamal B, Rathi N, Chauhan S, Jadon V, Vats N, Gehlot A, Arya S (2010). In vitro rapid and mass multiplication of highly valuable medicinal plant Bacopa monnieri (L.) Wettst. Afr. J. Biotechnol. 9:8318-8322.


APA (2013). Micropropagation of Litsea glutinosa (Lour) C.B. International Journal of Biotechnology and Molecular Biology Research, 4(5), 78-85.
Chicago Syed Naseer Shah, Amjad M. Husaini and S. A. Ansari. "Micropropagation of Litsea glutinosa (Lour) C.B." International Journal of Biotechnology and Molecular Biology Research 4, no. 5 (2013): 78-85.
MLA Syed Naseer Shah, Amjad M. Husaini and S. A. Ansari. "Micropropagation of Litsea glutinosa (Lour) C.B." International Journal of Biotechnology and Molecular Biology Research 4.5 (2013): 78-85.

Subscription Form