International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 97

Full Length Research Paper

Genetic variation of 17 Y-chromosomal short tandem repeats (STRs) loci from unrelated individuals in Iraq

Imad H.1*, Cheah Q.1, Mohammad J.2 and Aamera O.3
1Department of Molecular Biology, Putra University, Kuala Lumpur City, Malaysia. 2Department of Molecular Biology, Babylon University, Hilla City, Iraq. 3Institute of Medico-legal in Baghdad, Ministry of Health of Iraq, Iraq.
Email: [email protected]

  •  Accepted: 20 November 2013
  •  Published: 31 December 2013

Abstract

Analysis of 17 Y chromosomal STR loci in a population sample of 400 unrelated male living in the middle and south of Iraq was done to evaluate allele frequencies and gene diversity for each Y-STR locus of the Y filer™ PCR amplification kit. The seventeen loci include DYS635, DYS437, DYS448, DYS456, DYS458, YGATA H4, DYS389I, DYS389II, DYS19, DYS391, DYS438, DYS390, DYS439, DYS392, DYS393, DYS385a and DYS385b. Total DNA from blood cells was extracted using the FTA™ paper DNA extraction. A total of 361 unique haplotypes were identified among the four hundred individuals studied. The DYS385b had the highest diversity (GD = 0.8392), while loci DYS392 had the lowest (D = 0.2695).

Key words: Allele frequency, gene diversity, Iraq, Y filer™.