International Journal of Biotechnology and Molecular Biology Research
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Article Number - FFCF7AA60770


Vol.7(3), pp. 29-35 , September 2016
DOI: 10.5897/IJBMBR2012.018
ISSN: 2141-2154



Full Length Research Paper

Efficient somatic embryogenesis of Jatropha curcas L. from petiole and leaf discs



Cecilia Mbithe Mweu
  • Cecilia Mbithe Mweu
  • Jomo Kenyatta University of Agriculture Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Aggrey Nyende
  • Aggrey Nyende
  • Jomo Kenyatta University of Agriculture Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Justus Onguso
  • Justus Onguso
  • Jomo Kenyatta University of Agriculture Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar







 Received: 21 August 2012  Accepted: 19 September 2016  Published: 30 September 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Jatropha curcas L. is peculiar treasured tree species for its uses and considerable economic potential as a biofuel plant. Propagation using seeds is limited by low viability and germination hence unable to provide high quality planting material for sustainable use. To achieve mass production of J. curcas, regeneration from leaf discs and petioles using different treatments of plant growth regulators (PGRs) including 6- benzyl aminopurine (BAP), Kinetin (KIN), Indole -3-acetic acid (IAA) and Thiadiazuron (TDZ) was explored. Maximum callus formation efficiency (85.00%) and the shoot proliferation per explants (8.25) was observed on MS medium supplemented with 1.5 mg/L BAP, 0.6 mg/L KIN, 0.3 mg/L IAA and 0.1 mg/L TDZ. Rooting was induced from elongated shoots cultured on half strength Murashige and Skoog (MS) medium fortified with different regimes of indole-3-butyric acid (IBA) and Naphthalene acetic acid (NAA). MS supplemented with 3.5 mg/L IBA and 3.5 mg/L NAA gave optimum root formation of 2.5 cm on JCO4, JN1, JE4 and JRV1 accessions. The rooted plants were established in forest soil, sand and manure mixed in the ratio of 2: 1:1 in the green house with 20% survival rate. This successful in vitro regeneration is vital for seedling system for mass production.

Key words: Plant growth regulators, callus, shoot induction, root formation.

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APA Mweu, C. M., Nyende, A., & Onguso, J. (2016). Efficient somatic embryogenesis of Jatropha curcas L. from petiole and leaf discs. International Journal of Biotechnology and Molecular Biology Research, 7(3), 29-35.
Chicago Cecilia Mbithe Mweu, Aggrey Nyende and Justus Onguso. "Efficient somatic embryogenesis of Jatropha curcas L. from petiole and leaf discs." International Journal of Biotechnology and Molecular Biology Research 7, no. 3 (2016): 29-35.
MLA Cecilia Mbithe Mweu, Aggrey Nyende and Justus Onguso. "Efficient somatic embryogenesis of Jatropha curcas L. from petiole and leaf discs." International Journal of Biotechnology and Molecular Biology Research 7.3 (2016): 29-35.
   
DOI 10.5897/IJBMBR2012.018
URL http://academicjournals.org/journal/IJBMBR/article-abstract/FFCF7AA60770

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